Characteristics of T-cell immunity in patients with acute leukemia

E. N. Parovichnikova, I. V. Galtseva, I. A. Vorobyev, V. G. Savchenko

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Aim. To study a cytokine profile of cytotoxic T-cells and T-helpers at diagnosis of acute myeloid and lymphoblastic leukemia (AML and ALL) and at different stages of its treatment. Material and methods. T-cell population of peripheral blood lymphocytes was studied in 19 AML patients at diagnosis, 13 AML patients in remission, 5 AML patients in developing recurrence and 5 ALL patients at diagnosis. The control group consisted of 10 healthy donors. A mononuclear fraction of peripheral blood cells was stained with monoclonal antibodies. Surface expression of CD3, CD4, CD8 antigens was estimated with the use of flow cytofluorimeter FACS Calibur. Part of the cells were cultured for production of activated lymphocytes. The cytokine profile of T-cells was investigated according to the modified method of Rostaing et al. in the population of CD3+CD8+ and CD3+CD8-T-cells using the flow cytofluorimeter FACS Calibur. The results were processed with the programs CellQuest and WmMDI, Statistica Module Switcher. Results. The percentage of CD3+CD8+ cells producing interferon-gamma (IF-g) was the same in AML patients and donors. The percentage of CD3+CD8- cells (T-helpers) producing IF-g was similar in the patients at diagnosis and in complete remission being higher than in healthy donors. The number of CD3+CD8- cells producing IF-g was reduced in recurrence while the number of CD3+CD8+ and CD3+CD8- cells producing interleukin-4 (IL-4) increased. In the onset of the disease, ALL patients had significantly increased percentage of CD3+CD8- IL-4 cells. Conclusion. The results obtained evidence for different polarization of T-cell immunity in AML and ALL patients at the time of diagnosis, remission and recurrence: in the onset of AML there was an increase in the production of Th1 - proinflammatory cytokines, in ALL - in percent of Th-2 cytokines. In AML recurrence the balance of cytokines shifted in the direction of Th-2 response.

Original languageEnglish
Pages (from-to)18-25
Number of pages8
JournalTerapevticheskii Arkhiv
Volume78
Issue number7
Publication statusPublished - 2006
Externally publishedYes

Fingerprint

Immunity
Leukemia
T-Lymphocytes
Cytokines
Interferon-gamma
Recurrence
Tissue Donors
Helper-Inducer T-Lymphocytes
Interleukin-4
Lymphocytes
CD8 Antigens
CD4 Antigens
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Acute Myeloid Leukemia
Population
Cultured Cells
Blood Cells
Monoclonal Antibodies
Control Groups

Keywords

  • Acute leukemias
  • Antitumor immunity
  • T-cell immune response

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Parovichnikova, E. N., Galtseva, I. V., Vorobyev, I. A., & Savchenko, V. G. (2006). Characteristics of T-cell immunity in patients with acute leukemia. Terapevticheskii Arkhiv, 78(7), 18-25.

Characteristics of T-cell immunity in patients with acute leukemia. / Parovichnikova, E. N.; Galtseva, I. V.; Vorobyev, I. A.; Savchenko, V. G.

In: Terapevticheskii Arkhiv, Vol. 78, No. 7, 2006, p. 18-25.

Research output: Contribution to journalArticle

Parovichnikova, EN, Galtseva, IV, Vorobyev, IA & Savchenko, VG 2006, 'Characteristics of T-cell immunity in patients with acute leukemia', Terapevticheskii Arkhiv, vol. 78, no. 7, pp. 18-25.
Parovichnikova, E. N. ; Galtseva, I. V. ; Vorobyev, I. A. ; Savchenko, V. G. / Characteristics of T-cell immunity in patients with acute leukemia. In: Terapevticheskii Arkhiv. 2006 ; Vol. 78, No. 7. pp. 18-25.
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N2 - Aim. To study a cytokine profile of cytotoxic T-cells and T-helpers at diagnosis of acute myeloid and lymphoblastic leukemia (AML and ALL) and at different stages of its treatment. Material and methods. T-cell population of peripheral blood lymphocytes was studied in 19 AML patients at diagnosis, 13 AML patients in remission, 5 AML patients in developing recurrence and 5 ALL patients at diagnosis. The control group consisted of 10 healthy donors. A mononuclear fraction of peripheral blood cells was stained with monoclonal antibodies. Surface expression of CD3, CD4, CD8 antigens was estimated with the use of flow cytofluorimeter FACS Calibur. Part of the cells were cultured for production of activated lymphocytes. The cytokine profile of T-cells was investigated according to the modified method of Rostaing et al. in the population of CD3+CD8+ and CD3+CD8-T-cells using the flow cytofluorimeter FACS Calibur. The results were processed with the programs CellQuest and WmMDI, Statistica Module Switcher. Results. The percentage of CD3+CD8+ cells producing interferon-gamma (IF-g) was the same in AML patients and donors. The percentage of CD3+CD8- cells (T-helpers) producing IF-g was similar in the patients at diagnosis and in complete remission being higher than in healthy donors. The number of CD3+CD8- cells producing IF-g was reduced in recurrence while the number of CD3+CD8+ and CD3+CD8- cells producing interleukin-4 (IL-4) increased. In the onset of the disease, ALL patients had significantly increased percentage of CD3+CD8- IL-4 cells. Conclusion. The results obtained evidence for different polarization of T-cell immunity in AML and ALL patients at the time of diagnosis, remission and recurrence: in the onset of AML there was an increase in the production of Th1 - proinflammatory cytokines, in ALL - in percent of Th-2 cytokines. In AML recurrence the balance of cytokines shifted in the direction of Th-2 response.

AB - Aim. To study a cytokine profile of cytotoxic T-cells and T-helpers at diagnosis of acute myeloid and lymphoblastic leukemia (AML and ALL) and at different stages of its treatment. Material and methods. T-cell population of peripheral blood lymphocytes was studied in 19 AML patients at diagnosis, 13 AML patients in remission, 5 AML patients in developing recurrence and 5 ALL patients at diagnosis. The control group consisted of 10 healthy donors. A mononuclear fraction of peripheral blood cells was stained with monoclonal antibodies. Surface expression of CD3, CD4, CD8 antigens was estimated with the use of flow cytofluorimeter FACS Calibur. Part of the cells were cultured for production of activated lymphocytes. The cytokine profile of T-cells was investigated according to the modified method of Rostaing et al. in the population of CD3+CD8+ and CD3+CD8-T-cells using the flow cytofluorimeter FACS Calibur. The results were processed with the programs CellQuest and WmMDI, Statistica Module Switcher. Results. The percentage of CD3+CD8+ cells producing interferon-gamma (IF-g) was the same in AML patients and donors. The percentage of CD3+CD8- cells (T-helpers) producing IF-g was similar in the patients at diagnosis and in complete remission being higher than in healthy donors. The number of CD3+CD8- cells producing IF-g was reduced in recurrence while the number of CD3+CD8+ and CD3+CD8- cells producing interleukin-4 (IL-4) increased. In the onset of the disease, ALL patients had significantly increased percentage of CD3+CD8- IL-4 cells. Conclusion. The results obtained evidence for different polarization of T-cell immunity in AML and ALL patients at the time of diagnosis, remission and recurrence: in the onset of AML there was an increase in the production of Th1 - proinflammatory cytokines, in ALL - in percent of Th-2 cytokines. In AML recurrence the balance of cytokines shifted in the direction of Th-2 response.

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