Characterization of the antibiotic compound no. 70 produced by Streptomyces sp. IMV-70

Lyudmila P. Trenozhnikova, Almagul K. Khasenova, Assya S. Balgimbaeva, Galina B. Fedorova, Genrikh S. Katrukha, Nina L. Tokareva, Boo H. Kwa, Azliyati Azizan

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We describe the actinomycete strain IMV-70 isolated from the soils of Kazakhstan, which produces potent antibiotics with high levels of antibacterial activity. After the research of its morphological, chemotaxonomic, and cultural characteristics, the strain with potential to be developed further as a novel class of antibiotics with chemotherapeutics potential was identified as Streptomyces sp. IMV-70. In the process of fermentation, the strain Streptomyces spp. IMV-70 produces the antibiotic no. 70, which was isolated from the culture broth by extraction with organic solvents. Antibiotic compound no. 70 was purified and separated into individual components by HPLC, TLC, and column chromatography methods. The main component of the compound is the antibiotic 70-A, which was found to be identical to the peptolide etamycin A. Two other antibiotics 70-B and 70-C have never been described and therefore are new antibiotics. The physical-chemical and biological characteristics of these preparations were described and further researched. Determination of the optimal growth conditions to cultivate actinomycete-producer strain IMV-70 and development of methods to isolate, purify, and accumulate preparations of the new antibiotic no. 70 enable us to research further the potential of this new class of antibiotics.

Original languageEnglish
Article number594231
JournalThe Scientific World Journal
Volume2012
DOIs
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

Streptomyces
antibiotics
Anti-Bacterial Agents
Actinobacteria
Kazakhstan
Cultural Characteristics
Column chromatography
biological characteristics
Research
Fermentation
Organic solvents
chromatography
fermentation
Chromatography
Soil
High Pressure Liquid Chromatography
Soils
Growth

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Medicine(all)

Cite this

Trenozhnikova, L. P., Khasenova, A. K., Balgimbaeva, A. S., Fedorova, G. B., Katrukha, G. S., Tokareva, N. L., ... Azizan, A. (2012). Characterization of the antibiotic compound no. 70 produced by Streptomyces sp. IMV-70. The Scientific World Journal, 2012, [594231]. https://doi.org/10.1100/2012/594231

Characterization of the antibiotic compound no. 70 produced by Streptomyces sp. IMV-70. / Trenozhnikova, Lyudmila P.; Khasenova, Almagul K.; Balgimbaeva, Assya S.; Fedorova, Galina B.; Katrukha, Genrikh S.; Tokareva, Nina L.; Kwa, Boo H.; Azizan, Azliyati.

In: The Scientific World Journal, Vol. 2012, 594231, 2012.

Research output: Contribution to journalArticle

Trenozhnikova, LP, Khasenova, AK, Balgimbaeva, AS, Fedorova, GB, Katrukha, GS, Tokareva, NL, Kwa, BH & Azizan, A 2012, 'Characterization of the antibiotic compound no. 70 produced by Streptomyces sp. IMV-70', The Scientific World Journal, vol. 2012, 594231. https://doi.org/10.1100/2012/594231
Trenozhnikova LP, Khasenova AK, Balgimbaeva AS, Fedorova GB, Katrukha GS, Tokareva NL et al. Characterization of the antibiotic compound no. 70 produced by Streptomyces sp. IMV-70. The Scientific World Journal. 2012;2012. 594231. https://doi.org/10.1100/2012/594231
Trenozhnikova, Lyudmila P. ; Khasenova, Almagul K. ; Balgimbaeva, Assya S. ; Fedorova, Galina B. ; Katrukha, Genrikh S. ; Tokareva, Nina L. ; Kwa, Boo H. ; Azizan, Azliyati. / Characterization of the antibiotic compound no. 70 produced by Streptomyces sp. IMV-70. In: The Scientific World Journal. 2012 ; Vol. 2012.
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