Characterization of two splice variants of metastasis-associated human mts1 gene

Noona Ambartsumian, Svetlana Tarabykina, Mariam Grigorian, Eugene Tulchinsky, Egil Hulgaard, Georgii Georgiev, Eugene Lukanidin

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

The mtsl gene is one of the genes specifically expressed in mouse metastatic tumors and tumor cell lines. In this paper, we present data on cloning and sequencing of two variants of human mtsl cDNAs (hu-mtsl and hu-mtsl (rar)), as well as of the corresponding region in the human genome. Comparison of the genomic sequence with the sequence of the mtsl cDNAs demonstrates presence of two alternatively spliced variants of the mtsl in the human osteosarcoma cell line (OHS). The alternative splicing occurs within the 5′-untranslated region (UTR) of human mtsl pre-mRNA. Both splice variants, hu-mtsl and hu-mtsl (rar), retain similar stability in the cells, contain one open reading frame coding for the MTS1 protein and differ only slightly in their translational capacity. The splice variants demonstrate dramatic variations in the level of expression in different human tissues and in human tumor cell lines. Although we have not revealed substantial differences in the mode of action of the two splice variants in the cells, the observed tissue specificity of expression supports the notion that it plays an important role in determining the activity of mtsl in different tissues.

Original languageEnglish
Pages (from-to)125-130
Number of pages6
JournalGene
Volume159
Issue number1
DOIs
Publication statusPublished - Jun 14 1995
Externally publishedYes

Keywords

  • 5'RACE
  • alternative splicing
  • genomic DNA
  • mtsl cDNA
  • Nucleotide sequence
  • tissue-specificity

ASJC Scopus subject areas

  • Genetics

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