Chondromodulin I and pleiotrophin gene expression in bovine cartilage and epiphysis

Azliyati Azizan, Joanne U. Gaw, Prasanthi Govindraj, Hazel Tapp, Peter J. Neame

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Pleiotrophin and chondromodulin-I are low molecular weight proteins that are abundant (20 μg/g tissue) in fetal cartilage and difficult to detect in adult cartilage. We characterized their gene and protein expression patterns to gain a better understanding of their roles in the regulation of limb development and growth. In order to compare and contrast the relative amounts of the respective mRNA species within the developing epiphysis, a competitive PCR assay was developed. The results showed that the mRNAs for both proteins were abundant in fetal cartilage and while present in adult cartilage, were at 20-60-fold lower levels. Northern blotting revealed gradients of mRNA for both of these proteins in growth plate cartilage, with the highest levels in the resting zone, and the lowest in the hypertrophic zone. In contrast to pleiotrophin, chondromodulin-1 is down-regulated by retinoic acid with a pattern of expression similar to collagen type II and link protein, and may play a more specific role than pleiotrophin in modulating the chondrocyte phenotype. Copyright (C) 2000 Elsevier Science B.V./International Society of Matrix Biology.

Original languageEnglish
Pages (from-to)521-531
Number of pages11
JournalMatrix Biology
Volume19
Issue number6
DOIs
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Epiphyses
Cartilage
Gene Expression
Messenger RNA
Proteins
Growth Plate
Collagen Type II
Chondrocytes
Tretinoin
Growth and Development
Northern Blotting
Fetus
Extremities
Molecular Weight
pleiotrophin
Phenotype
Polymerase Chain Reaction

Keywords

  • Cartilage
  • Chondrocyte
  • Chondromodulin-I
  • Competitive PCR
  • Pleiotrophin

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Chondromodulin I and pleiotrophin gene expression in bovine cartilage and epiphysis. / Azizan, Azliyati; Gaw, Joanne U.; Govindraj, Prasanthi; Tapp, Hazel; Neame, Peter J.

In: Matrix Biology, Vol. 19, No. 6, 2000, p. 521-531.

Research output: Contribution to journalArticle

Azizan, Azliyati ; Gaw, Joanne U. ; Govindraj, Prasanthi ; Tapp, Hazel ; Neame, Peter J. / Chondromodulin I and pleiotrophin gene expression in bovine cartilage and epiphysis. In: Matrix Biology. 2000 ; Vol. 19, No. 6. pp. 521-531.
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