Circulating adiponectin levels, expression of adiponectin receptors, and methylation of adiponectin gene promoter in relation to Alzheimer’s disease

Aiym Kaiyrlykyzy, Bauyrzhan Umbayev, Abdul Razak Masoud, Aida Baibulatova, Andrey Tsoy, Farkhad Olzhayev, Dinara Alzhanova, Gulnaz Zholdasbekova, Kairat Davletov, Ainur Akilzhanova, Sholpan Askarova

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Background: The role of adiponectin (ADIPOQ) in Alzheimer’s disease (AD) has been documented, however, demonstrating controversial results. In this study, we investigated blood serum ADIPOQ levels, methylation of the adiponectin gene promoter, and adiponectin receptors (AdipoR1 and AdipoR2) expression in blood samples isolated from AD patients and healthy controls. Methods: We performed a case–control study including 248 subjects (98 AD patients and 150 healthy controls); ADIPOQ serum levels, AdipoR1, and AdipoR2 levels in PBMC were measured by ELISA Kits, and ADIPOQ gene methylation was analyzed using methyl-specific PCR. Results: Serum adiponectin levels were threefold higher in the AD group compared to the controls. We have also found a positive correlation between adiponectin and MMSE scores and high-density lipoprotein cholesterol (HDL-C) in AD patients. A significant difference in the proportion of methylation of the CpG sites at − 74 nt of the ADIPOQ gene promoter was detected in AD cases, and the levels of adiponectin in blood serum were significantly higher in methylated samples in the AD group compared to controls. The amount of AdipoR1 was significantly higher among AD subjects, while the expression of AdipoR2 did not vary between AD patients and controls. Conclusion: These findings may contribute to a deeper understanding of the etiological factors leading to the development of dementia and may serve as a basis for the development of predictive biomarkers of AD.

Original languageEnglish
Article number262
JournalBMC Medical Genomics
Volume15
Issue number1
DOIs
Publication statusPublished - Dec 2022

Keywords

  • Adiponectin
  • AdipoR1
  • AdipoR2
  • Alzheimer’s disease
  • DNA methylation

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Fingerprint

Dive into the research topics of 'Circulating adiponectin levels, expression of adiponectin receptors, and methylation of adiponectin gene promoter in relation to Alzheimer’s disease'. Together they form a unique fingerprint.

Cite this