CLASP1 and CLASP2 bind to EB1 and regulate microtubule plus-end dynamics at the cell cortex

Yuko Mimori-Kiyosue, Ilya Grigoriev, Gideon Lansbergen, Hiroyuki Sasaki, Chiyuki Matsui, Fedor Severin, Niels Galjart, Frank Grosveld, Ivan Vorobjev, Shoichiro Tsukita, Anna Akhmanova

Research output: Contribution to journalArticlepeer-review

295 Citations (Scopus)

Abstract

CLIP-associating protein (CLASP) 1 and CLASP2 are mammalian microtubule (MT) plus-end binding proteins, which associate with CLIP-170 and CLIP-115. Using RNA interference in HeLa cells, we show that the two CLASPs play redundant roles in regulating the density, length distribution and stability of interphase MTs. In HeLa cells, both CLASPs concentrate on the distal MT ends in a narrow region at the cell margin. CLASPs stabilize MTs by promoting pauses and restricting MT growth and shortening episodes to this peripheral cell region. We demonstrate that the middle part of CLASPs binds directly to EB1 and to MTs. Furthermore, we show that the association of CLASP2 with the cell cortex is MT independent and relies on its COOH-terminal domain. Both EB1- and cortex-binding domains of CLASP are required to promote MT stability. We propose that CLASPs can mediate interactions between MT plus ends and the cell cortex and act as local rescue factors, possibly through forming a complex with EB1 at MT tips.

Original languageEnglish
Pages (from-to)141-153
Number of pages13
JournalJournal of Cell Biology
Volume168
Issue number1
DOIs
Publication statusPublished - Jan 3 2005

ASJC Scopus subject areas

  • Cell Biology

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