Two hundred and thirty nine serial amniotic fluid specimens were obtained by serial amniocentesis from 91 patients in varying gestational ages. Lysozyme activity determination was based on the use of turbidimetry at 546 nm, calculated by a standard curve (phosphate buffer 67 mmol/l, pH 6.3, Micrococcus lysodeicticus 0.2 g/l, sodium azide 8 mmol/l), according to the method of Prockop and Davidson (Lysozyme - Testomer - Boehringwerke). Transferrin concentrations were estimated by the method of immunonephelometry using original Behring kits. All amniotic fluid samples were cultured for aerobic and anaerobic bacteria immediately after amniocentesis. Lysozyme concentration in amniotic fluid increased gradually with advancing gestational age from 2.50 mg/l in 13th week of gestation and reached maximum levels of 23.60 mg/l at term. Transferrin intraamniotic levels hightened in the similar pattern from 49.90 mg/l in 13th week and reached maximum levels of 260.30 mg/l in the 39 th gestational week. In the last week transferrin levels significantly decreased. Positive amniotic fluid culture after the second procedure was found in only one patient who had very low concentrations of these antimicrobial factors.
|Number of pages||6|
|Journal||Journal of Foetal Medicine|
|Publication status||Published - Dec 1 1992|
- amniotic fluid
- serial amniocentesis
ASJC Scopus subject areas
- Obstetrics and Gynaecology