Comparison of different serum sample extraction methods and their suitability for mass spectrometry analysis

Thamir M. Alshammari, Ahmed Ali Al-Hassan, Taibi B. Hadda, Mohamad Aljofan

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Mass spectrometry has been widely used, particularly in pharmacokinetic investigations and for therapeutic drug monitoring purposes. Like any other analytical method some difficulties exist in employing mass spectrometry, mainly when it is used to test biological samples, such as to detect drug candidates in mammalian serum, which is rich in proteins, lipids and other contents that may interfere with the investigational drug. The complexity of the serum proteome presents challenges for efficient sample preparation and adequate sensitivity for mass spectrometry analysis of drugs. Enrichment procedures prior to the drug analysis are often needed and as a result, the study of serum or plasma components usually demands either methods of purification or depletion of one or more. Selection of the best combination of sample introduction method is a crucial determinant of the sensitivity and accuracy of mass spectrometry. The aim of this study was to determine the highest serum protein precipitation activity of five commonly used sample preparation methods and test their suitability for mass spectrometry. We spiked three small molecules into rabbit serum and applied different protein precipitation methods to determine their precipitation activity and applicability as a mass spectrometry introductory tool.

Original languageEnglish
Pages (from-to)689-697
Number of pages9
JournalSaudi Pharmaceutical Journal
Volume23
Issue number6
DOIs
Publication statusPublished - Nov 1 2015
Externally publishedYes

Keywords

  • In-gel alkylation
  • Mass spectrometry
  • Protein extraction method
  • Serum sample

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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