Comparison of five artificial skin surface film liquids for assessing dermal bioaccessibility of metals in certified reference soils

Carlos A. Marin Villegas, Mert Guney, Gerald J. Zagury

Research output: Contribution to journalArticle

Abstract

© 2019 Dermal exposure to metals has previously received less attention than oral/inhalation exposure. Nonetheless, human health risk is significant for certain contaminants and exposure scenarios. The present study aims to (1) characterize two certified reference soils (SQC001, BGS 102); and (2) assess Cr, Ni, Pb, and Zn dermal bioaccessibility via in vitro assays using three synthetic sweat formulations (EN 1811, pH 6.5 (Sweat A), NIHS 96-10, pH 4.7 (Sweat B), and a more complex pH 5.5 formulation containing amino acids (Sweat C)) and two sebum formulations. Metals bioaccessibility in sweat followed Sweat B > Sweat C > Sweat A, attributed to sweat B lower pH. Dermal bioaccessibility in both sebum formulations was lower than 1% for Ni and Pb and below 9% for Cr and Zn, possibly due to low affinity of metals for non-polar lipids. It must be noted that bioaccessible Zn in BGS 102 was higher when extracted with synthetic sebum compared to any of the synthetic sweat formulations. Metal bioaccessibility in sweat was considerably higher for SQC001 (up to 76.6% for Zn using Sweat B) than for BGS 102 (ranging between 0.02 and 1.3% for all elements and all sweat formulations), attributed to higher pH, higher organic carbon, and higher cation exchange capacity of reference soil BGS 102. Sebum formulations spiked with metals generally entailed low metal recovery (except for Zn), which may explain overall low bioaccessibility values for sebum. Sebum and sweat formulation, and soil properties seem to control in vitro dermal bioaccessibility of metals.
Original languageEnglish
Pages (from-to)595-601
Number of pages7
JournalScience of the Total Environment
Volume692
DOIs
Publication statusPublished - Nov 20 2019

Fingerprint

Artificial Skin
Liquid films
skin
Skin
Metals
Soils
liquid
metal
soil
Metal recovery
Health risks
Organic carbon
Lipids
comparison
Cations
Amino acids
Assays
cation exchange capacity
Ion exchange
health risk

Keywords

  • Certified reference soils
  • Contaminated soils
  • Heavy metals
  • in vitro dermal bioaccessibility
  • Synthetic sebum
  • Synthetic sweat

Cite this

Comparison of five artificial skin surface film liquids for assessing dermal bioaccessibility of metals in certified reference soils. / Marin Villegas, Carlos A.; Guney, Mert; Zagury, Gerald J.

In: Science of the Total Environment, Vol. 692, 20.11.2019, p. 595-601.

Research output: Contribution to journalArticle

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