Comparison of flavins and a conjugated oligoelectrolyte in stimulating extracellular electron transport from Shewanella oneidensis MR-1

Victor Bochuan Wang, Nathan D. Kirchhofer, Xiaofen Chen, Melissa Yuan Li Tan, Krishnakumar Sivakumar, Bin Cao, Qichun Zhang, Staffan Kjelleberg, Guillermo C. Bazan, Say Chye Joachim Loo, Enrico Marsili

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

Slow extracellular electron transfer (EET) rates at the biofilm/electrode interface hinder the application of microbial bioelectronic technology in bioremediation as well as energy recovery from wastewater. Conjugated oligoelectrolytes (COEs) have been shown to increase EET in viable microorganisms. However, confirmation of these results on model electrochemically active microorganisms (EAMs), such as Shewanella oneidensis MR-1, is still lacking. Here, chemical modification of S. oneidensis is achieved through spontaneous intercalation of the amphiphilic water-soluble conjugated oligoelectrolyte, 4,4′-bis(4′-(N,N-bis(6″-(N,N,N- trimethylammonium)hexyl)amino)-styryl)stilbene tetraiodide (DSSN+). Various electrochemical techniques are used to compare the EET enhancement afforded to S. oneidensis by microbially produced flavins and addition of DSSN+. The effect of DSSN+ on the EET rate increases as the working electrode potential increases. However, DSSN+ addition did not fully restore the wild-type EET rate in MtrC-OmcA knockout mutants. These results drive the design of more effective COEs that will serve as an exact molecular surrogate for outer membrane cytochromes.

Original languageEnglish
Pages (from-to)55-58
Number of pages4
JournalElectrochemistry Communications
Volume41
DOIs
Publication statusPublished - Apr 2014
Externally publishedYes

Keywords

  • Conjugated oligoelectrolytes
  • Extracellular electron transfer
  • Shewanella oneidensis

ASJC Scopus subject areas

  • Electrochemistry

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