Composite cryogel with polyelectrolyte complexes for growth factor delivery

Arman Saparov, Bolat Sultankulov, Dmitriy Berillo, Sholpan Kauanova, Sergey Mikhalovsky, Lyuba Mikhalovska

Research output: Contribution to journalReview articlepeer-review

34 Citations (Scopus)

Abstract

Macroporous scaffolds composed of chitosan (CHI), hydroxyapatite (HA), heparin (Hep), and polyvinyl alcohol (PVA) were prepared with a glutaraldehyde (GA) cross-linker by cryogelation. Addition of PVA to the reaction mixture slowed down the formation of a polyelectrolyte complex (PEC) between CHI and Hep, which allowed more thorough mixing, and resulted in the development of the homogeneous matrix structure. Freezing of the CHI-HA-GA and PVA-Hep-GA mixture led to the formation of a non-stoichiometric PEC between oppositely charged groups of CHI and Hep, which caused further efficient immobilization of bone morphogenic protein 2 (BMP-2) possible due to electrostatic interactions. It was shown that the obtained cryogel matrix released BMP-2 and supported the differentiation of rat bone marrow mesenchymal stem cells (rat BMSCs) into the osteogenic lineage. Rat BMSCs attached to cryogel loaded with BMP-2 and expressed osteocalcin in vitro. Obtained composite cryogel with PEC may have high potential for bone regeneration and tissue engineering applications.

Original languageEnglish
Article number650
JournalPharmaceutics
Volume11
Issue number12
DOIs
Publication statusPublished - Dec 2019

Keywords

  • Biomaterials
  • Bone regeneration
  • Cryogel
  • Drug delivery
  • Growth factors
  • Tissue engineering

ASJC Scopus subject areas

  • Pharmaceutical Science

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