Conformational activation of radixin by G13 protein α subunit

Rita Vaiskunaite, Vyacheslav Adarichev, Heinz Furthmayr, Tohru Kozasa, Andrei Gudkov, Tatyana A. Voyno-Yasenetskaya

Research output: Contribution to journalArticle

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Abstract

G13 protein, one of the heterotrimeric guanine nucleotide-binding proteins (G proteins), regulates diverse and complex cellular responses by transducing signals from the cell surface presumably involving more than one pathway. Yeast two-hybrid screening of a mouse brain cDNA library identified radixin, a member of the ERM family of three closely related proteins (ezrin, radixin, and moesin), as a protein that interacted with Gα13. Interaction between radixin and Gα13 was confirmed by in vitro binding assay and by co-immunoprecipitation technique. Activated Gα13 induced conformational activation of radixin, as determined by binding of radixin to polymerized F-actin and by immunofluorescence in intact cells. Finally, two dominant negative mutants of radixin inhibited Gα13-induced focus formation of Rat-1 fibroblasts but did not affect Ras-induced focus formation. Our resets identifying a new signaling pathway for Gα13 indicate that ERM proteins can be activated by and serve as effectors of heterotrimeric G proteins.

Original languageEnglish
Pages (from-to)26206-26212
Number of pages7
JournalJournal of Biological Chemistry
Volume275
Issue number34
DOIs
Publication statusPublished - Aug 25 2000
Externally publishedYes

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G12-G13 GTP-Binding Protein alpha Subunits
Protein Subunits
Chemical activation
Heterotrimeric GTP-Binding Proteins
Proteins
Guanine Nucleotides
Fibroblasts
Gene Library
GTP-Binding Proteins
Immunoprecipitation
Yeast
Fluorescent Antibody Technique
radixin
Rats
Actins
Assays
Brain
Carrier Proteins
Screening
Yeasts

ASJC Scopus subject areas

  • Biochemistry

Cite this

Vaiskunaite, R., Adarichev, V., Furthmayr, H., Kozasa, T., Gudkov, A., & Voyno-Yasenetskaya, T. A. (2000). Conformational activation of radixin by G13 protein α subunit. Journal of Biological Chemistry, 275(34), 26206-26212. https://doi.org/10.1074/jbc.M001863200

Conformational activation of radixin by G13 protein α subunit. / Vaiskunaite, Rita; Adarichev, Vyacheslav; Furthmayr, Heinz; Kozasa, Tohru; Gudkov, Andrei; Voyno-Yasenetskaya, Tatyana A.

In: Journal of Biological Chemistry, Vol. 275, No. 34, 25.08.2000, p. 26206-26212.

Research output: Contribution to journalArticle

Vaiskunaite, R, Adarichev, V, Furthmayr, H, Kozasa, T, Gudkov, A & Voyno-Yasenetskaya, TA 2000, 'Conformational activation of radixin by G13 protein α subunit', Journal of Biological Chemistry, vol. 275, no. 34, pp. 26206-26212. https://doi.org/10.1074/jbc.M001863200
Vaiskunaite R, Adarichev V, Furthmayr H, Kozasa T, Gudkov A, Voyno-Yasenetskaya TA. Conformational activation of radixin by G13 protein α subunit. Journal of Biological Chemistry. 2000 Aug 25;275(34):26206-26212. https://doi.org/10.1074/jbc.M001863200
Vaiskunaite, Rita ; Adarichev, Vyacheslav ; Furthmayr, Heinz ; Kozasa, Tohru ; Gudkov, Andrei ; Voyno-Yasenetskaya, Tatyana A. / Conformational activation of radixin by G13 protein α subunit. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 34. pp. 26206-26212.
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