Conservation of heterochromatin protein 1 function

G Wang, A Ma, C M Chow, D Horsley, N R Brown, I G Cowell, P B Singh

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Heterochromatin represents a cytologically visible state of heritable gene repression. In the yeast, Schizosaccharomyces pombe, the swi6 gene encodes a heterochromatin protein 1 (HP1)-like chromodomain protein that localizes to heterochromatin domains, including the centromeres, telomeres, and the donor mating-type loci, and is involved in silencing at these loci. We identify here the functional domains of swi6p and demonstrate that the chromodomain from a mammalian HP1-like protein, M31, can functionally replace that of swi6p, showing that chromodomain function is conserved from yeasts to humans. Site-directed mutagenesis, based on a modeled three-dimensional structure of the swi6p chromodomain, shows that the hydrophobic amino acids which lie in the core of the structure are critical for biological function. Gel filtration, gel overlay experiments, and mass spectroscopy show that HP1 proteins can self-associate, and we suggest that it is as oligomers that HP1 proteins are incorporated into heterochromatin complexes that silence gene activity.

Original languageEnglish
Pages (from-to)6970-83
Number of pages14
JournalMolecular and Cellular Biology
Volume20
Issue number18
Publication statusPublished - Sep 2000

Fingerprint

Heterochromatin
Proteins
Yeasts
Genes
Schizosaccharomyces
Centromere
Telomere
Site-Directed Mutagenesis
Gel Chromatography
Mass Spectrometry
Gels
Amino Acids
heterochromatin-specific nonhistone chromosomal protein HP-1
like heterochromatin protein 1

Keywords

  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • Chromosomal Proteins, Non-Histone
  • Chromosomes, Fungal
  • Evolution, Molecular
  • Female
  • Fungal Proteins
  • Heterochromatin
  • Humans
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Nuclear Localization Signals
  • Protein Structure, Quaternary
  • Rats
  • Rats, Inbred F344
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces
  • Transcription Factors
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Wang, G., Ma, A., Chow, C. M., Horsley, D., Brown, N. R., Cowell, I. G., & Singh, P. B. (2000). Conservation of heterochromatin protein 1 function. Molecular and Cellular Biology, 20(18), 6970-83.

Conservation of heterochromatin protein 1 function. / Wang, G; Ma, A; Chow, C M; Horsley, D; Brown, N R; Cowell, I G; Singh, P B.

In: Molecular and Cellular Biology, Vol. 20, No. 18, 09.2000, p. 6970-83.

Research output: Contribution to journalArticle

Wang, G, Ma, A, Chow, CM, Horsley, D, Brown, NR, Cowell, IG & Singh, PB 2000, 'Conservation of heterochromatin protein 1 function', Molecular and Cellular Biology, vol. 20, no. 18, pp. 6970-83.
Wang G, Ma A, Chow CM, Horsley D, Brown NR, Cowell IG et al. Conservation of heterochromatin protein 1 function. Molecular and Cellular Biology. 2000 Sep;20(18):6970-83.
Wang, G ; Ma, A ; Chow, C M ; Horsley, D ; Brown, N R ; Cowell, I G ; Singh, P B. / Conservation of heterochromatin protein 1 function. In: Molecular and Cellular Biology. 2000 ; Vol. 20, No. 18. pp. 6970-83.
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