TY - JOUR
T1 - Continuous antigenic stimulation system (CASS) as a new immunization strategy
AU - Gomez-Vargas, Andrew
AU - Rosenthal, Kenneth L.
AU - McDermott, Mark R.
AU - Hortelano, Gonzalo
N1 - Funding Information:
We would like to thank Dr. Scott W. Gallichan for excellent technical advice with the lymphoproliferation assay, Dr. Yonghong Wan for kindly providing MHC-I KO mice, and Dr. Frederick A. Ofosu for generous supply of Benefix. KLR is the recipient of a Career Scientist Award from the Ontario HIV Treatment Network (OHTN). This work was funded in part by a grant from the Canadian Blood Services to GH.
PY - 2004/9/28
Y1 - 2004/9/28
N2 - Protection against diseases is mediated by a sustained immune response. Here, we describe a new immunization strategy. Mice implanted with encapsulated C2C12 myoblasts secreting human factor IX (hFIX) elicited a strong humoral response against the transgene, as compared to mice immunized with complete Freund's adjuvant (FA). Mice also had increasing IgG2a antibody titer, indicating a switch to a Th1 profile immune response. Mice developed strong hFIX-specific cytotoxic T lymphocytes (CTL) that was detectable 213 days after implantation, demonstrating the sustained immunity elicited by encapsulated cells. Here, we propose continuous antigenic stimulation system (CASS) as a novel immunization strategy with potential application in the design of novel vaccines.
AB - Protection against diseases is mediated by a sustained immune response. Here, we describe a new immunization strategy. Mice implanted with encapsulated C2C12 myoblasts secreting human factor IX (hFIX) elicited a strong humoral response against the transgene, as compared to mice immunized with complete Freund's adjuvant (FA). Mice also had increasing IgG2a antibody titer, indicating a switch to a Th1 profile immune response. Mice developed strong hFIX-specific cytotoxic T lymphocytes (CTL) that was detectable 213 days after implantation, demonstrating the sustained immunity elicited by encapsulated cells. Here, we propose continuous antigenic stimulation system (CASS) as a novel immunization strategy with potential application in the design of novel vaccines.
KW - Antigen stimulation system
KW - Immune response
KW - Immunization strategy
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U2 - 10.1016/j.vaccine.2004.04.010
DO - 10.1016/j.vaccine.2004.04.010
M3 - Article
C2 - 15364438
AN - SCOPUS:4444358396
SN - 0264-410X
VL - 22
SP - 3902
EP - 3910
JO - Vaccine
JF - Vaccine
IS - 29-30
ER -