Continuous antigenic stimulation system (CASS) as a new immunization strategy

Andrew Gomez-Vargas, Kenneth L. Rosenthal, Mark R. McDermott, Gonzalo Hortelano

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Protection against diseases is mediated by a sustained immune response. Here, we describe a new immunization strategy. Mice implanted with encapsulated C2C12 myoblasts secreting human factor IX (hFIX) elicited a strong humoral response against the transgene, as compared to mice immunized with complete Freund's adjuvant (FA). Mice also had increasing IgG2a antibody titer, indicating a switch to a Th1 profile immune response. Mice developed strong hFIX-specific cytotoxic T lymphocytes (CTL) that was detectable 213 days after implantation, demonstrating the sustained immunity elicited by encapsulated cells. Here, we propose continuous antigenic stimulation system (CASS) as a novel immunization strategy with potential application in the design of novel vaccines.

Original languageEnglish
Pages (from-to)3902-3910
Number of pages9
JournalVaccine
Volume22
Issue number29-30
DOIs
Publication statusPublished - Sep 28 2004
Externally publishedYes

Fingerprint

Immunization
immunization
Factor IX
mice
immune response
myoblasts
cytotoxic T-lymphocytes
Freund's Adjuvant
Myoblasts
Cytotoxic T-Lymphocytes
Transgenes
humoral immunity
transgenes
adjuvants
Immunity
Vaccines
immunity
vaccines
antibodies
Antibodies

Keywords

  • Antigen stimulation system
  • Immune response
  • Immunization strategy

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Continuous antigenic stimulation system (CASS) as a new immunization strategy. / Gomez-Vargas, Andrew; Rosenthal, Kenneth L.; McDermott, Mark R.; Hortelano, Gonzalo.

In: Vaccine, Vol. 22, No. 29-30, 28.09.2004, p. 3902-3910.

Research output: Contribution to journalArticle

Gomez-Vargas, Andrew ; Rosenthal, Kenneth L. ; McDermott, Mark R. ; Hortelano, Gonzalo. / Continuous antigenic stimulation system (CASS) as a new immunization strategy. In: Vaccine. 2004 ; Vol. 22, No. 29-30. pp. 3902-3910.
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