Could nuclear matrix protein 22 (NMP22) play a role with urine cytology in screening for bladder cancer? - Experience at Kuwait University

K. Kapila, E. O. Kehinde, J. T. Anim, O. A. Mojiminiyi, A. Vinsu, S. S. George, M. Al-Maghrebi, F. Al-Mulla

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objectives: This prospective study was undertaken to evaluate nuclear matrix protein (NMP22) compared to urine cytology in the detection of bladder cancer and also to determine whether indexing suspicious cytology to NMP22 could enhance the clinical utility of cytology. Methods: Cytological findings of voided urine collected prior to a cystoscopic biopsy were correlated with urine NMP22 assay in 46 patients attending the urology clinic in Mubarak Al-Kabeer Hospital. The patients were clinically categorized into newly diagnosed cases of transitional cell carcinoma (TCC), recurrent TCC, TCC in remission and controls. Results: Using histological diagnosis as the gold standard the sensitivity and specificity of NMP22 were 78% and 43% respectively and of cases with malignant urine cytology were 30% and 87% respectively. If suspicious and malignant cytology were combined as positive results the sensitivity increased significantly to 87% while the specificity decreased but not significantly to 74%. Suspicious or malignant cytology enhanced by positive NMP22 gave a sensitivity of 70% and specificity of 87% neither of which was significantly different from cytology alone. There were three false positive cases on cytology and 13 false positive cases on NMP22 assay. There were three false negative cytology and five false negative NMP22 cases but only one was false negative for both, resulting in a high sensitivity (96%) but low specificity (30%) if either positive NMP22 or malignant or suspicious cytology was taken as a positive result. Conclusion: Combining NMP22 with malignant or suspicious cytological result improved sensitivity for the detection of bladder cancer but with a major decrease in specificity, suggesting a potential role in screening rather than diagnosis.

Original languageEnglish
Pages (from-to)369-374
Number of pages6
JournalCytopathology
Volume19
Issue number6
DOIs
Publication statusPublished - Dec 2008
Externally publishedYes

Fingerprint

Kuwait
Urinary Bladder Neoplasms
Cell Biology
Urine
Transitional Cell Carcinoma
Nuclear Matrix-Associated Proteins
nuclear matrix protein 22
Sensitivity and Specificity
Urology
Prospective Studies

Keywords

  • Bladder cancer
  • NMP22
  • Sensitivity
  • Specificity
  • Suspicious cytology
  • Urine cytology

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

Cite this

Could nuclear matrix protein 22 (NMP22) play a role with urine cytology in screening for bladder cancer? - Experience at Kuwait University. / Kapila, K.; Kehinde, E. O.; Anim, J. T.; Mojiminiyi, O. A.; Vinsu, A.; George, S. S.; Al-Maghrebi, M.; Al-Mulla, F.

In: Cytopathology, Vol. 19, No. 6, 12.2008, p. 369-374.

Research output: Contribution to journalArticle

Kapila, K, Kehinde, EO, Anim, JT, Mojiminiyi, OA, Vinsu, A, George, SS, Al-Maghrebi, M & Al-Mulla, F 2008, 'Could nuclear matrix protein 22 (NMP22) play a role with urine cytology in screening for bladder cancer? - Experience at Kuwait University', Cytopathology, vol. 19, no. 6, pp. 369-374. https://doi.org/10.1111/j.1365-2303.2008.00586.x
Kapila, K. ; Kehinde, E. O. ; Anim, J. T. ; Mojiminiyi, O. A. ; Vinsu, A. ; George, S. S. ; Al-Maghrebi, M. ; Al-Mulla, F. / Could nuclear matrix protein 22 (NMP22) play a role with urine cytology in screening for bladder cancer? - Experience at Kuwait University. In: Cytopathology. 2008 ; Vol. 19, No. 6. pp. 369-374.
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abstract = "Objectives: This prospective study was undertaken to evaluate nuclear matrix protein (NMP22) compared to urine cytology in the detection of bladder cancer and also to determine whether indexing suspicious cytology to NMP22 could enhance the clinical utility of cytology. Methods: Cytological findings of voided urine collected prior to a cystoscopic biopsy were correlated with urine NMP22 assay in 46 patients attending the urology clinic in Mubarak Al-Kabeer Hospital. The patients were clinically categorized into newly diagnosed cases of transitional cell carcinoma (TCC), recurrent TCC, TCC in remission and controls. Results: Using histological diagnosis as the gold standard the sensitivity and specificity of NMP22 were 78{\%} and 43{\%} respectively and of cases with malignant urine cytology were 30{\%} and 87{\%} respectively. If suspicious and malignant cytology were combined as positive results the sensitivity increased significantly to 87{\%} while the specificity decreased but not significantly to 74{\%}. Suspicious or malignant cytology enhanced by positive NMP22 gave a sensitivity of 70{\%} and specificity of 87{\%} neither of which was significantly different from cytology alone. There were three false positive cases on cytology and 13 false positive cases on NMP22 assay. There were three false negative cytology and five false negative NMP22 cases but only one was false negative for both, resulting in a high sensitivity (96{\%}) but low specificity (30{\%}) if either positive NMP22 or malignant or suspicious cytology was taken as a positive result. Conclusion: Combining NMP22 with malignant or suspicious cytological result improved sensitivity for the detection of bladder cancer but with a major decrease in specificity, suggesting a potential role in screening rather than diagnosis.",
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AU - Vinsu, A.

AU - George, S. S.

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AU - Al-Mulla, F.

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