Abstract
apoptosis can be stimulated by various factors: biological, chemical and physical. We investigated cytotoxic effect of combination
of pharmacological apoptosis modulator Velcade (Janssen-Cilag Pty Ltd) and hypethermia. The cytotoxicity was studied for 5
concentrations of Velcade (1, 2, 3, 4 and 5 ng/ml), three types of temperature (40, 42 and 44°C), and two types of cancer cells (human
melanoma C-32 and human monocytic leukaemia U937 lines). The results demonstrated that Velcade in all 5 concentrations was
able of suppress the viability of U937 cells. The decrease in cell survival rate was in a direct correlation with the increase of drug’s
concentration. The maximal level of dead cells was detected for 40°C for all concentrations of Velcade. However, the acquired data
showed that the augmentation of temperature up to 42°C and 44°C resulted in the rise of number of viable cells. These findings
indicate that high temperatures have a cyto-protective effect. The possible mechanism may lay in the ability of mild hyperthermia to
promote cell growth. The second feasible explanation is a negative impact of high temperature on Velcade’s pharmacodynamics. The
data of cytotoxicity studies of C-32 cells showed a similar picture. The elevation of temperature led to the decrease of cells viability.
Notably, the highest temperature (44°C) had a cyto-protective effect too. In conclusion, our findings indicate that combination of
apoptosis modulator Velcade and mild hypethermia can be effectively employed for the elimination of cancer cells.
| Original language | English |
|---|---|
| Title of host publication | Cytotoxicity studies of combination of proteasome inhibitor Velcade and hyperthermia |
| Place of Publication | Cologne, Germany |
| Number of pages | 1 |
| DOIs | |
| Publication status | Published - 2016 |
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Cytotoxicity studies of combination of proteasome inhibitor Velcade and hyperthermia. / Baiskhanova, Dinara; Saliev, Timur; Begimbetova, Dinara; Feril, Loreto B.; Merinyanu, Xeniya; Tachibana, Katsuro.
Cytotoxicity studies of combination of proteasome inhibitor Velcade and hyperthermia. Cologne, Germany, 2016.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Cytotoxicity studies of combination of proteasome inhibitor Velcade and hyperthermia
AU - Baiskhanova, Dinara
AU - Saliev, Timur
AU - Begimbetova, Dinara
AU - Feril, Loreto B.
AU - Merinyanu, Xeniya
AU - Tachibana, Katsuro
PY - 2016
Y1 - 2016
N2 - One of perspective methods of cancer treatment is the induction of apoptosis (‘programmed cell death’) in malignant cells. Theapoptosis can be stimulated by various factors: biological, chemical and physical. We investigated cytotoxic effect of combinationof pharmacological apoptosis modulator Velcade (Janssen-Cilag Pty Ltd) and hypethermia. The cytotoxicity was studied for 5concentrations of Velcade (1, 2, 3, 4 and 5 ng/ml), three types of temperature (40, 42 and 44°C), and two types of cancer cells (humanmelanoma C-32 and human monocytic leukaemia U937 lines). The results demonstrated that Velcade in all 5 concentrations wasable of suppress the viability of U937 cells. The decrease in cell survival rate was in a direct correlation with the increase of drug’sconcentration. The maximal level of dead cells was detected for 40°C for all concentrations of Velcade. However, the acquired datashowed that the augmentation of temperature up to 42°C and 44°C resulted in the rise of number of viable cells. These findingsindicate that high temperatures have a cyto-protective effect. The possible mechanism may lay in the ability of mild hyperthermia topromote cell growth. The second feasible explanation is a negative impact of high temperature on Velcade’s pharmacodynamics. Thedata of cytotoxicity studies of C-32 cells showed a similar picture. The elevation of temperature led to the decrease of cells viability.Notably, the highest temperature (44°C) had a cyto-protective effect too. In conclusion, our findings indicate that combination ofapoptosis modulator Velcade and mild hypethermia can be effectively employed for the elimination of cancer cells.
AB - One of perspective methods of cancer treatment is the induction of apoptosis (‘programmed cell death’) in malignant cells. Theapoptosis can be stimulated by various factors: biological, chemical and physical. We investigated cytotoxic effect of combinationof pharmacological apoptosis modulator Velcade (Janssen-Cilag Pty Ltd) and hypethermia. The cytotoxicity was studied for 5concentrations of Velcade (1, 2, 3, 4 and 5 ng/ml), three types of temperature (40, 42 and 44°C), and two types of cancer cells (humanmelanoma C-32 and human monocytic leukaemia U937 lines). The results demonstrated that Velcade in all 5 concentrations wasable of suppress the viability of U937 cells. The decrease in cell survival rate was in a direct correlation with the increase of drug’sconcentration. The maximal level of dead cells was detected for 40°C for all concentrations of Velcade. However, the acquired datashowed that the augmentation of temperature up to 42°C and 44°C resulted in the rise of number of viable cells. These findingsindicate that high temperatures have a cyto-protective effect. The possible mechanism may lay in the ability of mild hyperthermia topromote cell growth. The second feasible explanation is a negative impact of high temperature on Velcade’s pharmacodynamics. Thedata of cytotoxicity studies of C-32 cells showed a similar picture. The elevation of temperature led to the decrease of cells viability.Notably, the highest temperature (44°C) had a cyto-protective effect too. In conclusion, our findings indicate that combination ofapoptosis modulator Velcade and mild hypethermia can be effectively employed for the elimination of cancer cells.
U2 - http://dx.doi.org/10.4172/1948-5956.C1.070
DO - http://dx.doi.org/10.4172/1948-5956.C1.070
M3 - Conference contribution
BT - Cytotoxicity studies of combination of proteasome inhibitor Velcade and hyperthermia
CY - Cologne, Germany
ER -