Cytotoxicity studies of combination of proteasome inhibitor Velcade and hyperthermia

Dinara Baiskhanova, Timur Saliev, Dinara Begimbetova, Loreto B. Feril, Xeniya Merinyanu, Katsuro Tachibana

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

One of perspective methods of cancer treatment is the induction of apoptosis (‘programmed cell death’) in malignant cells. The
apoptosis can be stimulated by various factors: biological, chemical and physical. We investigated cytotoxic effect of combination
of pharmacological apoptosis modulator Velcade (Janssen-Cilag Pty Ltd) and hypethermia. The cytotoxicity was studied for 5
concentrations of Velcade (1, 2, 3, 4 and 5 ng/ml), three types of temperature (40, 42 and 44°C), and two types of cancer cells (human
melanoma C-32 and human monocytic leukaemia U937 lines). The results demonstrated that Velcade in all 5 concentrations was
able of suppress the viability of U937 cells. The decrease in cell survival rate was in a direct correlation with the increase of drug’s
concentration. The maximal level of dead cells was detected for 40°C for all concentrations of Velcade. However, the acquired data
showed that the augmentation of temperature up to 42°C and 44°C resulted in the rise of number of viable cells. These findings
indicate that high temperatures have a cyto-protective effect. The possible mechanism may lay in the ability of mild hyperthermia to
promote cell growth. The second feasible explanation is a negative impact of high temperature on Velcade’s pharmacodynamics. The
data of cytotoxicity studies of C-32 cells showed a similar picture. The elevation of temperature led to the decrease of cells viability.
Notably, the highest temperature (44°C) had a cyto-protective effect too. In conclusion, our findings indicate that combination of
apoptosis modulator Velcade and mild hypethermia can be effectively employed for the elimination of cancer cells.
Original languageEnglish
Title of host publicationCytotoxicity studies of combination of proteasome inhibitor Velcade and hyperthermia
Place of PublicationCologne, Germany
Number of pages1
DOIs
Publication statusPublished - 2016

Fingerprint

Proteasome Inhibitors
Fever
Temperature
Cell Survival
Apoptosis
Neoplasms
U937 Cells
Biological Factors
Bortezomib
Leukemia
Cell Death
Cell Count
Pharmacology
Growth

Cite this

Baiskhanova, D., Saliev, T., Begimbetova, D., Feril, L. B., Merinyanu, X., & Tachibana, K. (2016). Cytotoxicity studies of combination of proteasome inhibitor Velcade and hyperthermia. In Cytotoxicity studies of combination of proteasome inhibitor Velcade and hyperthermia Cologne, Germany. https://doi.org/10.4172/1948-5956.C1.070

Cytotoxicity studies of combination of proteasome inhibitor Velcade and hyperthermia. / Baiskhanova, Dinara; Saliev, Timur; Begimbetova, Dinara; Feril, Loreto B.; Merinyanu, Xeniya; Tachibana, Katsuro.

Cytotoxicity studies of combination of proteasome inhibitor Velcade and hyperthermia. Cologne, Germany, 2016.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Baiskhanova, D, Saliev, T, Begimbetova, D, Feril, LB, Merinyanu, X & Tachibana, K 2016, Cytotoxicity studies of combination of proteasome inhibitor Velcade and hyperthermia. in Cytotoxicity studies of combination of proteasome inhibitor Velcade and hyperthermia. Cologne, Germany. https://doi.org/10.4172/1948-5956.C1.070
Baiskhanova D, Saliev T, Begimbetova D, Feril LB, Merinyanu X, Tachibana K. Cytotoxicity studies of combination of proteasome inhibitor Velcade and hyperthermia. In Cytotoxicity studies of combination of proteasome inhibitor Velcade and hyperthermia. Cologne, Germany. 2016 https://doi.org/10.4172/1948-5956.C1.070
Baiskhanova, Dinara ; Saliev, Timur ; Begimbetova, Dinara ; Feril, Loreto B. ; Merinyanu, Xeniya ; Tachibana, Katsuro. / Cytotoxicity studies of combination of proteasome inhibitor Velcade and hyperthermia. Cytotoxicity studies of combination of proteasome inhibitor Velcade and hyperthermia. Cologne, Germany, 2016.
@inproceedings{8e3b6b1e30d34b76a31ce70053bd532f,
title = "Cytotoxicity studies of combination of proteasome inhibitor Velcade and hyperthermia",
abstract = "One of perspective methods of cancer treatment is the induction of apoptosis (‘programmed cell death’) in malignant cells. Theapoptosis can be stimulated by various factors: biological, chemical and physical. We investigated cytotoxic effect of combinationof pharmacological apoptosis modulator Velcade (Janssen-Cilag Pty Ltd) and hypethermia. The cytotoxicity was studied for 5concentrations of Velcade (1, 2, 3, 4 and 5 ng/ml), three types of temperature (40, 42 and 44°C), and two types of cancer cells (humanmelanoma C-32 and human monocytic leukaemia U937 lines). The results demonstrated that Velcade in all 5 concentrations wasable of suppress the viability of U937 cells. The decrease in cell survival rate was in a direct correlation with the increase of drug’sconcentration. The maximal level of dead cells was detected for 40°C for all concentrations of Velcade. However, the acquired datashowed that the augmentation of temperature up to 42°C and 44°C resulted in the rise of number of viable cells. These findingsindicate that high temperatures have a cyto-protective effect. The possible mechanism may lay in the ability of mild hyperthermia topromote cell growth. The second feasible explanation is a negative impact of high temperature on Velcade’s pharmacodynamics. Thedata of cytotoxicity studies of C-32 cells showed a similar picture. The elevation of temperature led to the decrease of cells viability.Notably, the highest temperature (44°C) had a cyto-protective effect too. In conclusion, our findings indicate that combination ofapoptosis modulator Velcade and mild hypethermia can be effectively employed for the elimination of cancer cells.",
author = "Dinara Baiskhanova and Timur Saliev and Dinara Begimbetova and Feril, {Loreto B.} and Xeniya Merinyanu and Katsuro Tachibana",
year = "2016",
doi = "http://dx.doi.org/10.4172/1948-5956.C1.070",
language = "English",
booktitle = "Cytotoxicity studies of combination of proteasome inhibitor Velcade and hyperthermia",

}

TY - GEN

T1 - Cytotoxicity studies of combination of proteasome inhibitor Velcade and hyperthermia

AU - Baiskhanova, Dinara

AU - Saliev, Timur

AU - Begimbetova, Dinara

AU - Feril, Loreto B.

AU - Merinyanu, Xeniya

AU - Tachibana, Katsuro

PY - 2016

Y1 - 2016

N2 - One of perspective methods of cancer treatment is the induction of apoptosis (‘programmed cell death’) in malignant cells. Theapoptosis can be stimulated by various factors: biological, chemical and physical. We investigated cytotoxic effect of combinationof pharmacological apoptosis modulator Velcade (Janssen-Cilag Pty Ltd) and hypethermia. The cytotoxicity was studied for 5concentrations of Velcade (1, 2, 3, 4 and 5 ng/ml), three types of temperature (40, 42 and 44°C), and two types of cancer cells (humanmelanoma C-32 and human monocytic leukaemia U937 lines). The results demonstrated that Velcade in all 5 concentrations wasable of suppress the viability of U937 cells. The decrease in cell survival rate was in a direct correlation with the increase of drug’sconcentration. The maximal level of dead cells was detected for 40°C for all concentrations of Velcade. However, the acquired datashowed that the augmentation of temperature up to 42°C and 44°C resulted in the rise of number of viable cells. These findingsindicate that high temperatures have a cyto-protective effect. The possible mechanism may lay in the ability of mild hyperthermia topromote cell growth. The second feasible explanation is a negative impact of high temperature on Velcade’s pharmacodynamics. Thedata of cytotoxicity studies of C-32 cells showed a similar picture. The elevation of temperature led to the decrease of cells viability.Notably, the highest temperature (44°C) had a cyto-protective effect too. In conclusion, our findings indicate that combination ofapoptosis modulator Velcade and mild hypethermia can be effectively employed for the elimination of cancer cells.

AB - One of perspective methods of cancer treatment is the induction of apoptosis (‘programmed cell death’) in malignant cells. Theapoptosis can be stimulated by various factors: biological, chemical and physical. We investigated cytotoxic effect of combinationof pharmacological apoptosis modulator Velcade (Janssen-Cilag Pty Ltd) and hypethermia. The cytotoxicity was studied for 5concentrations of Velcade (1, 2, 3, 4 and 5 ng/ml), three types of temperature (40, 42 and 44°C), and two types of cancer cells (humanmelanoma C-32 and human monocytic leukaemia U937 lines). The results demonstrated that Velcade in all 5 concentrations wasable of suppress the viability of U937 cells. The decrease in cell survival rate was in a direct correlation with the increase of drug’sconcentration. The maximal level of dead cells was detected for 40°C for all concentrations of Velcade. However, the acquired datashowed that the augmentation of temperature up to 42°C and 44°C resulted in the rise of number of viable cells. These findingsindicate that high temperatures have a cyto-protective effect. The possible mechanism may lay in the ability of mild hyperthermia topromote cell growth. The second feasible explanation is a negative impact of high temperature on Velcade’s pharmacodynamics. Thedata of cytotoxicity studies of C-32 cells showed a similar picture. The elevation of temperature led to the decrease of cells viability.Notably, the highest temperature (44°C) had a cyto-protective effect too. In conclusion, our findings indicate that combination ofapoptosis modulator Velcade and mild hypethermia can be effectively employed for the elimination of cancer cells.

U2 - http://dx.doi.org/10.4172/1948-5956.C1.070

DO - http://dx.doi.org/10.4172/1948-5956.C1.070

M3 - Conference contribution

BT - Cytotoxicity studies of combination of proteasome inhibitor Velcade and hyperthermia

CY - Cologne, Germany

ER -