Deliganding carbonic adsorbents for simultaneous removal of protein-bound toxins, bacterial toxins and inflammatory cytokines

V. G. Nikolaev, V. V. Sarnatskaya, A. N. Sidorenko, K. I. Bardakhivskaya, E. A. Snezhkova, L. A. Yushko, V. N. Maslenny, L. A. Sakhno, S. V. Mikhalovsky, O. P. Kozynchenko, A. V. Nikolaev

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

In this work structural and sorption properties of granulated carbonic sorbents obtained by deep pyrolysis of synthetic resins, are described. It has been shown that these sorbents possess high (up to 2.7 cm3 g -1) pore volume, low (up to 0.06 g cm-3) bulk density, well developed supermicro- and mesoporosity and significantly fractal structure in the range from 75 to 900 Å. Upon contact with solutions of human serum albumin (HSA), they efficiently adsorb protein-bound ligands with association constants from 103 to 108 M-1. These sorbents coded as HSGD (from HemoSorbent Granulated Deliganding) demonstrate adsorption capacity for unconjugated bilirubin, fatty and bile acids, phenols, CMPF, hippuric acid and indoxyl sulfate, which is tenfold higher than that for conventional granulated synthetic carbon hemosorbents. At the same time capacity of HSGD for freely soluble low molecular weight compounds, such as creatinine and methylene blue increases insignificantly. Simultaneously HSGD demonstrates high adsorption capacity for inflammatory cytokines TNF-α, IL-1, IL-6 that could be further enhanced by coating of the carbonic surface with native DNA or dextrane sulfate. Hemocompatibility of HSGD may be significantly increased by adding citrate to blood or by coating its surface with serum albumin. Finally, HSGD capacity coated with HSA for bacterial lipopolysaccharide (LPS) is by 15-20% higher than that of uncoated one. The use of certain molecular or pH-conformers of HSA for HSGD coating creates a diffusion " transparent" layer that practically does not affect the adsorption capacity of the carbonic matrix towards protein-bound ligands. It has been demonstrated that the deep pyrolysis technology used to produce HSGD can be applied to other carbonic sorbents made from granular synthetic, natural and fibrous raw materials.

Original languageEnglish
Title of host publicationNATO Science for Peace and Security Series A: Chemistry and Biology
Pages289-305
Number of pages17
DOIs
Publication statusPublished - 2011
Externally publishedYes

Publication series

NameNATO Science for Peace and Security Series A: Chemistry and Biology
ISSN (Print)18746489

Fingerprint

Bacterial Toxins
Sorbents
Serum Albumin
Adsorbents
Cytokines
Adsorption
Proteins
Coatings
Pyrolysis
Indican
Ligands
Synthetic Resins
Synthetic resins
Fractals
Acids
Phenols
Methylene Blue
Bile Acids and Salts
Interleukin-1
Bilirubin

Keywords

  • Activated carbon
  • Blirubin
  • Hemoperfusion
  • Protein bound toxins
  • Uremic toxins

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Safety, Risk, Reliability and Quality

Cite this

Nikolaev, V. G., Sarnatskaya, V. V., Sidorenko, A. N., Bardakhivskaya, K. I., Snezhkova, E. A., Yushko, L. A., ... Nikolaev, A. V. (2011). Deliganding carbonic adsorbents for simultaneous removal of protein-bound toxins, bacterial toxins and inflammatory cytokines. In NATO Science for Peace and Security Series A: Chemistry and Biology (pp. 289-305). (NATO Science for Peace and Security Series A: Chemistry and Biology). https://doi.org/10.1007/978-94-007-0217-2-29

Deliganding carbonic adsorbents for simultaneous removal of protein-bound toxins, bacterial toxins and inflammatory cytokines. / Nikolaev, V. G.; Sarnatskaya, V. V.; Sidorenko, A. N.; Bardakhivskaya, K. I.; Snezhkova, E. A.; Yushko, L. A.; Maslenny, V. N.; Sakhno, L. A.; Mikhalovsky, S. V.; Kozynchenko, O. P.; Nikolaev, A. V.

NATO Science for Peace and Security Series A: Chemistry and Biology. 2011. p. 289-305 (NATO Science for Peace and Security Series A: Chemistry and Biology).

Research output: Chapter in Book/Report/Conference proceedingChapter

Nikolaev, VG, Sarnatskaya, VV, Sidorenko, AN, Bardakhivskaya, KI, Snezhkova, EA, Yushko, LA, Maslenny, VN, Sakhno, LA, Mikhalovsky, SV, Kozynchenko, OP & Nikolaev, AV 2011, Deliganding carbonic adsorbents for simultaneous removal of protein-bound toxins, bacterial toxins and inflammatory cytokines. in NATO Science for Peace and Security Series A: Chemistry and Biology. NATO Science for Peace and Security Series A: Chemistry and Biology, pp. 289-305. https://doi.org/10.1007/978-94-007-0217-2-29
Nikolaev VG, Sarnatskaya VV, Sidorenko AN, Bardakhivskaya KI, Snezhkova EA, Yushko LA et al. Deliganding carbonic adsorbents for simultaneous removal of protein-bound toxins, bacterial toxins and inflammatory cytokines. In NATO Science for Peace and Security Series A: Chemistry and Biology. 2011. p. 289-305. (NATO Science for Peace and Security Series A: Chemistry and Biology). https://doi.org/10.1007/978-94-007-0217-2-29
Nikolaev, V. G. ; Sarnatskaya, V. V. ; Sidorenko, A. N. ; Bardakhivskaya, K. I. ; Snezhkova, E. A. ; Yushko, L. A. ; Maslenny, V. N. ; Sakhno, L. A. ; Mikhalovsky, S. V. ; Kozynchenko, O. P. ; Nikolaev, A. V. / Deliganding carbonic adsorbents for simultaneous removal of protein-bound toxins, bacterial toxins and inflammatory cytokines. NATO Science for Peace and Security Series A: Chemistry and Biology. 2011. pp. 289-305 (NATO Science for Peace and Security Series A: Chemistry and Biology).
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AU - Snezhkova, E. A.

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