@inbook{f9b627f2baa445e98f151e2e4964ff76,
title = "Designing degenerate primers: Overview, challenges, and computational methods",
abstract = "This chapter provides an overview of the concepts, challenges, and computational methods for designing degenerate primers, an important tool in molecular biology for amplifying various gene homologues and variable genomes. It also delves into the intricacies of the primer selection problem and provides a step-by-step approach, utilizing three advanced bioinformatics tools such as Gemi and RPrimer to create primers targeting the HSP70 gene in different species. The approach can be used to design degenerate primers for any gene with any intended downstream PCR applications, such as gene amplification, gene identification, molecular diagnostics, etc.",
keywords = "Computational methods and tools, Degenerate primers, PCR, Primer design",
author = "Dina Kalinina and Abidi, \{Syed Hani\}",
note = "Publisher Copyright: {\textcopyright} 2025 Elsevier Ltd",
year = "2025",
doi = "10.1016/bs.mim.2025.01.002",
language = "English",
series = "Methods in Microbiology",
publisher = "Academic Press Inc.",
booktitle = "Methods in Microbiology",
address = "United States",
}