Detection of bovine leukemia provirus by polymerase chain reaction followed by non-radioactive blot hybridization

A. N. Popov, V. A. Adarichev, S. M. Kalachikov, E. S. Mishina, G. M. Dymshits

Research output: Contribution to journalArticle

Abstract

A multiprobe scheme for detecting provirus of bovine leukemia virus (BLV) in the peripheral blood of infected animals was developed. According to the scheme, a fragment of BLV X-gene was amplified by polymerase chain reaction and detected by blot hybridization with the biotinilated oligonucleotide probe complementary to the inner part of the generated fragment. 600 copies of provirus may be specifically detected in a sample containing 150,000 cells. In some cases, BLV was detected in blood samples negative if tested by the commercial double immunodiffusion test.

Original languageEnglish
Pages (from-to)113-116
Number of pages4
JournalVoprosy Virusologii
Volume38
Issue number3
Publication statusPublished - 1993
Externally publishedYes

Fingerprint

Enzootic Bovine Leukosis
Bovine Leukemia Virus
Proviruses
Polymerase Chain Reaction
Oligonucleotide Probes
Immunodiffusion
Genes

ASJC Scopus subject areas

  • Virology

Cite this

Popov, A. N., Adarichev, V. A., Kalachikov, S. M., Mishina, E. S., & Dymshits, G. M. (1993). Detection of bovine leukemia provirus by polymerase chain reaction followed by non-radioactive blot hybridization. Voprosy Virusologii, 38(3), 113-116.

Detection of bovine leukemia provirus by polymerase chain reaction followed by non-radioactive blot hybridization. / Popov, A. N.; Adarichev, V. A.; Kalachikov, S. M.; Mishina, E. S.; Dymshits, G. M.

In: Voprosy Virusologii, Vol. 38, No. 3, 1993, p. 113-116.

Research output: Contribution to journalArticle

Popov, AN, Adarichev, VA, Kalachikov, SM, Mishina, ES & Dymshits, GM 1993, 'Detection of bovine leukemia provirus by polymerase chain reaction followed by non-radioactive blot hybridization', Voprosy Virusologii, vol. 38, no. 3, pp. 113-116.
Popov, A. N. ; Adarichev, V. A. ; Kalachikov, S. M. ; Mishina, E. S. ; Dymshits, G. M. / Detection of bovine leukemia provirus by polymerase chain reaction followed by non-radioactive blot hybridization. In: Voprosy Virusologii. 1993 ; Vol. 38, No. 3. pp. 113-116.
@article{f63f3130a1be43f486069fd6150ee23d,
title = "Detection of bovine leukemia provirus by polymerase chain reaction followed by non-radioactive blot hybridization",
abstract = "A multiprobe scheme for detecting provirus of bovine leukemia virus (BLV) in the peripheral blood of infected animals was developed. According to the scheme, a fragment of BLV X-gene was amplified by polymerase chain reaction and detected by blot hybridization with the biotinilated oligonucleotide probe complementary to the inner part of the generated fragment. 600 copies of provirus may be specifically detected in a sample containing 150,000 cells. In some cases, BLV was detected in blood samples negative if tested by the commercial double immunodiffusion test.",
author = "Popov, {A. N.} and Adarichev, {V. A.} and Kalachikov, {S. M.} and Mishina, {E. S.} and Dymshits, {G. M.}",
year = "1993",
language = "English",
volume = "38",
pages = "113--116",
journal = "Voprosy Virusologii",
issn = "0507-4088",
publisher = "Izdatelstvo Meditsina",
number = "3",

}

TY - JOUR

T1 - Detection of bovine leukemia provirus by polymerase chain reaction followed by non-radioactive blot hybridization

AU - Popov, A. N.

AU - Adarichev, V. A.

AU - Kalachikov, S. M.

AU - Mishina, E. S.

AU - Dymshits, G. M.

PY - 1993

Y1 - 1993

N2 - A multiprobe scheme for detecting provirus of bovine leukemia virus (BLV) in the peripheral blood of infected animals was developed. According to the scheme, a fragment of BLV X-gene was amplified by polymerase chain reaction and detected by blot hybridization with the biotinilated oligonucleotide probe complementary to the inner part of the generated fragment. 600 copies of provirus may be specifically detected in a sample containing 150,000 cells. In some cases, BLV was detected in blood samples negative if tested by the commercial double immunodiffusion test.

AB - A multiprobe scheme for detecting provirus of bovine leukemia virus (BLV) in the peripheral blood of infected animals was developed. According to the scheme, a fragment of BLV X-gene was amplified by polymerase chain reaction and detected by blot hybridization with the biotinilated oligonucleotide probe complementary to the inner part of the generated fragment. 600 copies of provirus may be specifically detected in a sample containing 150,000 cells. In some cases, BLV was detected in blood samples negative if tested by the commercial double immunodiffusion test.

UR - http://www.scopus.com/inward/record.url?scp=0027598893&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027598893&partnerID=8YFLogxK

M3 - Article

VL - 38

SP - 113

EP - 116

JO - Voprosy Virusologii

JF - Voprosy Virusologii

SN - 0507-4088

IS - 3

ER -