Detection of bovine leukemia provirus by polymerase chain reaction followed by non-radioactive blot hybridization

A. N. Popov, V. A. Adarichev, S. M. Kalachikov, E. S. Mishina, G. M. Dymshits

Research output: Contribution to journalArticlepeer-review

Abstract

A multiprobe scheme for detecting provirus of bovine leukemia virus (BLV) in the peripheral blood of infected animals was developed. According to the scheme, a fragment of BLV X-gene was amplified by polymerase chain reaction and detected by blot hybridization with the biotinilated oligonucleotide probe complementary to the inner part of the generated fragment. 600 copies of provirus may be specifically detected in a sample containing 150,000 cells. In some cases, BLV was detected in blood samples negative if tested by the commercial double immunodiffusion test.

Original languageEnglish
Pages (from-to)113-116
Number of pages4
JournalVoprosy Virusologii
Volume38
Issue number3
Publication statusPublished - Jan 1 1993

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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