Detection of Lactobacillus species using a gene fragment of the RNA polymerase beta subunit rpoB

A. B. Shevtsov, A. R. Kushugulova, S. S. Kojakhmetov, S. S. Oralbaeva, L. G. Stoyanova, A. B. Abzhalelov, A. B. Seidalina, K. T. Momynaliev

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

In this paper, we report on the comparative analysis of nucleotide sequences of the rpoB gene to assess its potential for use as a genetic marker for identification of Lactibacillus spp. recovered from acid milk products in different regions of Kazakhstan. The discriminatory power of the rpoB gene sequence was shown to be significantly higher for identification of closely related species of the Lactobacillus genus as compared to that of the 16S rRNA gene. The phylogeny based on the rpoB gene proved identical to that of the 16S rRNA gene and could be used as a supplement phylogenetic marker.

Original languageEnglish
Pages (from-to)22-27
Number of pages6
JournalMoscow University Biological Sciences Bulletin
Volume66
Issue number1
DOIs
Publication statusPublished - Mar 1 2011

Keywords

  • 16S rRNA gene
  • Lactobacillus
  • RNA polymerase beta subunit
  • identification
  • phylogeny
  • rpoB gene
  • sequencing

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Agricultural and Biological Sciences(all)

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