Detection of Lactobacillus species using a gene fragment of the RNA polymerase beta subunit rpoB

A. B. Shevtsov; A. R. Kushugulova; Samat Kozhakhmetov; S. S. Oralbaeva; L. G. Stoyanova; A. B. Abzhalelov; A. B. Seidalina; K. T. Momynaliev

doi:10.3103/S0096392511010093

Detection of Lactobacillus species using a gene fragment of the RNA polymerase beta subunit rpoB

A. B. Shevtsov, A. R. Kushugulova, Samat Kozhakhmetov, S. S. Oralbaeva, L. G. Stoyanova, A. B. Abzhalelov, A. B. Seidalina, K. T. Momynaliev

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

In this paper, we report on the comparative analysis of nucleotide sequences of the rpoB gene to assess its potential for use as a genetic marker for identification of Lactibacillus spp. recovered from acid milk products in different regions of Kazakhstan. The discriminatory power of the rpoB gene sequence was shown to be significantly higher for identification of closely related species of the Lactobacillus genus as compared to that of the 16S rRNA gene. The phylogeny based on the rpoB gene proved identical to that of the 16S rRNA gene and could be used as a supplement phylogenetic marker.

Original language	English
Pages (from-to)	22-27
Number of pages	6
Journal	Moscow University Biological Sciences Bulletin
Volume	66
Issue number	1
DOIs	https://doi.org/10.3103/S0096392511010093
Publication status	Published - Mar 2011

Keywords

16S rRNA gene
Lactobacillus
RNA polymerase beta subunit
identification
phylogeny
rpoB gene
sequencing

ASJC Scopus subject areas

General Biochemistry,Genetics and Molecular Biology
General Environmental Science
General Agricultural and Biological Sciences

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10.3103/S0096392511010093

Cite this

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title = "Detection of Lactobacillus species using a gene fragment of the RNA polymerase beta subunit rpoB",

abstract = "In this paper, we report on the comparative analysis of nucleotide sequences of the rpoB gene to assess its potential for use as a genetic marker for identification of Lactibacillus spp. recovered from acid milk products in different regions of Kazakhstan. The discriminatory power of the rpoB gene sequence was shown to be significantly higher for identification of closely related species of the Lactobacillus genus as compared to that of the 16S rRNA gene. The phylogeny based on the rpoB gene proved identical to that of the 16S rRNA gene and could be used as a supplement phylogenetic marker.",

keywords = "16S rRNA gene, Lactobacillus, RNA polymerase beta subunit, identification, phylogeny, rpoB gene, sequencing",

author = "Shevtsov, {A. B.} and Kushugulova, {A. R.} and Samat Kozhakhmetov and Oralbaeva, {S. S.} and Stoyanova, {L. G.} and Abzhalelov, {A. B.} and Seidalina, {A. B.} and Momynaliev, {K. T.}",

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AU - Shevtsov, A. B.

AU - Kushugulova, A. R.

AU - Kozhakhmetov, Samat

AU - Oralbaeva, S. S.

AU - Stoyanova, L. G.

AU - Abzhalelov, A. B.

AU - Seidalina, A. B.

AU - Momynaliev, K. T.

PY - 2011/3

Y1 - 2011/3

N2 - In this paper, we report on the comparative analysis of nucleotide sequences of the rpoB gene to assess its potential for use as a genetic marker for identification of Lactibacillus spp. recovered from acid milk products in different regions of Kazakhstan. The discriminatory power of the rpoB gene sequence was shown to be significantly higher for identification of closely related species of the Lactobacillus genus as compared to that of the 16S rRNA gene. The phylogeny based on the rpoB gene proved identical to that of the 16S rRNA gene and could be used as a supplement phylogenetic marker.

AB - In this paper, we report on the comparative analysis of nucleotide sequences of the rpoB gene to assess its potential for use as a genetic marker for identification of Lactibacillus spp. recovered from acid milk products in different regions of Kazakhstan. The discriminatory power of the rpoB gene sequence was shown to be significantly higher for identification of closely related species of the Lactobacillus genus as compared to that of the 16S rRNA gene. The phylogeny based on the rpoB gene proved identical to that of the 16S rRNA gene and could be used as a supplement phylogenetic marker.

KW - 16S rRNA gene

KW - Lactobacillus

KW - RNA polymerase beta subunit

KW - identification

KW - phylogeny

KW - rpoB gene

KW - sequencing

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Detection of Lactobacillus species using a gene fragment of the RNA polymerase beta subunit rpoB

Abstract

Keywords

ASJC Scopus subject areas

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