Detection of thrombin protein with ball lens microresonator fabricated using CO2laser splicing system

Takhmina Ayupova, Madina Shaimerdenova, Aliya Bekmurzayeva, Marzhan Sypabekova, Arman Aitkulov, Daniele Tosi

Research output: Chapter in Book/Report/Conference proceedingConference contribution


In this work, the investigation of thrombin concentration using microresonator functionalized with thiol-modified thrombin-binding aptamer (TBA) is reported. Resonator is a ball shaped structure on a tip of an optical fiber. Thrombin is a serine protease that plays an important role during blood coagulation; therefore, it is substantial to detect its levels in blood of the patient. The structure has been manufactured using standard single-mode fibers on CO2 laser splicing system. Further, the resonator of diameter 518 μm has been calibrated with sucrose for refractive index changes with optical backscatter reflectometer. Changes in sensitivity and reflectivity in wavelength shift and amplitude fluctuation were measured. This was followed by coating the resonator with gold and functionalizing surface with TBA. Following aptamer immobilization, the performance of 518 μm resonator was examined in different concentrations of thrombin protein from 4.01 nM to 66.84 nM. We report here a sample presenting a sensitivity of (122.65 nm/RIU, RIU = refractive index units), which allows thrombin detection with an average wavelength shift of 1.034 nm.

Original languageEnglish
Title of host publicationDesign and Quality for Biomedical Technologies XIV
EditorsJeeseong Hwang, Gracie Vargas, T. Joshua Pfefer
ISBN (Electronic)9781510641013
Publication statusPublished - 2021
EventDesign and Quality for Biomedical Technologies XIV 2021 - Virtual, Online, United States
Duration: Mar 6 2021Mar 11 2021

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceDesign and Quality for Biomedical Technologies XIV 2021
Country/TerritoryUnited States
CityVirtual, Online


  • Aptamer
  • Ball resonator
  • Biosensor
  • COlaser splicing system
  • Optical backscatter reflectometry
  • Optical fiber biosensors
  • Optical resonators
  • Thrombin

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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