Abstract
The response of etched fiber Bragg grating (EFBG) functionalized with 29-mer DNA aptamer to the different concentrations of Thrombin protein has been investigated. Etched FBGs are an efficient technology for detection of refractive index, and have been demonstrated also for biosensors applications. EFBGs have a simpler manufacturing approach comparing to other methodologies and are based on a low-cost device; their fabrication can be achieved by simple chemical etching, without requiring fusion splicing. During the test we assessed its feasibility for small variations of thrombin concentrations (10μg/ml, 20μg/ml, 40μg/ml and 80μg/ml). In particular, we performed experiments of chemical etching with hydrofluoric acid, which progressively depletes the fiber cladding exposing the core to the outer medium. Additionally, unstriped not etched FBGs were also used as a control for temperature pattern compensation. Before functionalization, EFBG was calibrated with different sucrose and ethanol solutions that validated the sensitivity to refractive index change. EFBG was further silanized with 3-Aminopropyl-triethoxysilane (APTES) in order to immobilize Thrombin binding aptamer on the silica surface of the fiber. The change of Bragg wavelength when functionalized EFBG is exposed to different concentrations of Thrombin using Micron Optics Hyperion si255-x55 sensing system was demonstrated. A small yet detectable sensitivity (several tens of nanomolars) even between small protein variations allows hypothesizing a future use of this kind of functionalized fiber for biosensor development.
| Original language | English |
|---|---|
| Title of host publication | Optics in Health Care and Biomedical Optics VIII |
| Editors | Ying Gu, Qingming Luo, Xingde Li, Yuguo Tang, Dan Zhu |
| Publisher | SPIE |
| Volume | 10820 |
| ISBN (Electronic) | 9781510622388 |
| DOIs | |
| Publication status | Published - Jan 1 2018 |
| Event | Optics in Health Care and Biomedical Optics VIII 2018 - Beijing, China Duration: Oct 11 2018 → Oct 13 2018 |
Conference
| Conference | Optics in Health Care and Biomedical Optics VIII 2018 |
|---|---|
| Country | China |
| City | Beijing |
| Period | 10/11/18 → 10/13/18 |
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Keywords
- aptamer
- biosensor
- etching
- fiber Bragg grating (FBG)
- optical fiber
- refractive index
- thrombin
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Computer Science Applications
- Applied Mathematics
- Electrical and Electronic Engineering
Cite this
Detection of various Thrombin concentrations using etched fiber Bragg gratings functionalized with DNA aptamer. / Shaimerdenova, M.; Bekmurzayeva, A.; Ayupova, T.; Sypabekova, M.; Korganbayev, S.; Dukenbayev, K.; Molardi, C.; Tosi, D.
Optics in Health Care and Biomedical Optics VIII. ed. / Ying Gu; Qingming Luo; Xingde Li; Yuguo Tang; Dan Zhu. Vol. 10820 SPIE, 2018. 108200O.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Detection of various Thrombin concentrations using etched fiber Bragg gratings functionalized with DNA aptamer
AU - Shaimerdenova, M.
AU - Bekmurzayeva, A.
AU - Ayupova, T.
AU - Sypabekova, M.
AU - Korganbayev, S.
AU - Dukenbayev, K.
AU - Molardi, C.
AU - Tosi, D.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - The response of etched fiber Bragg grating (EFBG) functionalized with 29-mer DNA aptamer to the different concentrations of Thrombin protein has been investigated. Etched FBGs are an efficient technology for detection of refractive index, and have been demonstrated also for biosensors applications. EFBGs have a simpler manufacturing approach comparing to other methodologies and are based on a low-cost device; their fabrication can be achieved by simple chemical etching, without requiring fusion splicing. During the test we assessed its feasibility for small variations of thrombin concentrations (10μg/ml, 20μg/ml, 40μg/ml and 80μg/ml). In particular, we performed experiments of chemical etching with hydrofluoric acid, which progressively depletes the fiber cladding exposing the core to the outer medium. Additionally, unstriped not etched FBGs were also used as a control for temperature pattern compensation. Before functionalization, EFBG was calibrated with different sucrose and ethanol solutions that validated the sensitivity to refractive index change. EFBG was further silanized with 3-Aminopropyl-triethoxysilane (APTES) in order to immobilize Thrombin binding aptamer on the silica surface of the fiber. The change of Bragg wavelength when functionalized EFBG is exposed to different concentrations of Thrombin using Micron Optics Hyperion si255-x55 sensing system was demonstrated. A small yet detectable sensitivity (several tens of nanomolars) even between small protein variations allows hypothesizing a future use of this kind of functionalized fiber for biosensor development.
AB - The response of etched fiber Bragg grating (EFBG) functionalized with 29-mer DNA aptamer to the different concentrations of Thrombin protein has been investigated. Etched FBGs are an efficient technology for detection of refractive index, and have been demonstrated also for biosensors applications. EFBGs have a simpler manufacturing approach comparing to other methodologies and are based on a low-cost device; their fabrication can be achieved by simple chemical etching, without requiring fusion splicing. During the test we assessed its feasibility for small variations of thrombin concentrations (10μg/ml, 20μg/ml, 40μg/ml and 80μg/ml). In particular, we performed experiments of chemical etching with hydrofluoric acid, which progressively depletes the fiber cladding exposing the core to the outer medium. Additionally, unstriped not etched FBGs were also used as a control for temperature pattern compensation. Before functionalization, EFBG was calibrated with different sucrose and ethanol solutions that validated the sensitivity to refractive index change. EFBG was further silanized with 3-Aminopropyl-triethoxysilane (APTES) in order to immobilize Thrombin binding aptamer on the silica surface of the fiber. The change of Bragg wavelength when functionalized EFBG is exposed to different concentrations of Thrombin using Micron Optics Hyperion si255-x55 sensing system was demonstrated. A small yet detectable sensitivity (several tens of nanomolars) even between small protein variations allows hypothesizing a future use of this kind of functionalized fiber for biosensor development.
KW - aptamer
KW - biosensor
KW - etching
KW - fiber Bragg grating (FBG)
KW - optical fiber
KW - refractive index
KW - thrombin
UR - http://www.scopus.com/inward/record.url?scp=85057292951&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85057292951&partnerID=8YFLogxK
U2 - 10.1117/12.2502457
DO - 10.1117/12.2502457
M3 - Conference contribution
AN - SCOPUS:85057292951
VL - 10820
BT - Optics in Health Care and Biomedical Optics VIII
A2 - Gu, Ying
A2 - Luo, Qingming
A2 - Li, Xingde
A2 - Tang, Yuguo
A2 - Zhu, Dan
PB - SPIE
ER -