Development and validation of a simple and sensitive HPLC method for the determination of liquid form of therapeutic substances

Background: Caffeine is the most consumed psychostimulant in the world that is found in numerous foods and drinks with coffee and tea have the highest concentration of caffeine per weight. Caffeine has a number of reported physiological benefits and clinical uses such as coma recovery. Caffeine can prove dangerous and its consumption, the amount in foods and drinks should be closely monitored, by the Food and Drug Administration. Material and Methods: The availability of sensitive, reliable, and simple testing methods with rapid turnover time is essential for testing any laboratories. In this manuscript, we describe the development and validation of a simple, sensitive and economical high performance liquid chromatographic (HPLC) method with ultraviolet detection for the quantification of the amount of caffeine in different beverages. Results: HPLC method was validated and tested to measure caffeine contents in different beverages that showed high precision, reliability, and sensitivity that makes it suitable for routine measurement of caffeine as well as other therapeutic or chemicals in liquid forms. Conclusions: The proposed method recovered caffeine without the need for any extraction step for recovering the caffeine from the formulation excipients matrices, therefore decreased the degree of error, time for estimation of caffeine and the overall cost of the analysis.