[Development of immunochromatographic test system for the rapid detection of lipopolysaccharide antigen and cells of causative agent of bovine brucellosis].

N. A. Byzova, A. V. Zherdev, S. Z. Eskendirova, K. K. Baltin, G. B. Unysheva, K. K. Mukanov, E. M. Ramankulov, B. B. Dzantiev

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A rapid method for detection of the surface lipopolysaccharide antigen and the cells of the causative agent of bovine brucellosis was developed. The method represents a sandwich format immunochromatographic assay. The contact between the sample and the test strip with immobilized immunoreagents initiates the fluid movement along the membrane components of the test strip, immunochemical reactions, and the formation of colored bands. The novel method requires 10 minutes to determine the lipopolysaccharide antigen of the cell wall of the brucellosis causative agent at concentrations down to 10 ng/mL and the Brucella abortus cells at concentrations down to 10(6) cells/mL (5 x 10(4) cells in the sample). The specificity of the immunodetection was confirmed. The designed test system can be used for the rapid field diagnosis of brucellosis in cattle.

Original languageEnglish
Pages (from-to)653-661
Number of pages9
JournalPrikladnaia biokhimiia i mikrobiologiia
Volume48
Issue number6
Publication statusPublished - Nov 2012
Externally publishedYes

Fingerprint

Bovine Brucellosis
Lipopolysaccharides
Brucellosis
Antigens
Immunochromatography
Brucella abortus
Assays
Cells
Surface Antigens
Membranes
Cell Wall
Fluids

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Byzova, N. A., Zherdev, A. V., Eskendirova, S. Z., Baltin, K. K., Unysheva, G. B., Mukanov, K. K., ... Dzantiev, B. B. (2012). [Development of immunochromatographic test system for the rapid detection of lipopolysaccharide antigen and cells of causative agent of bovine brucellosis]. Prikladnaia biokhimiia i mikrobiologiia, 48(6), 653-661.

[Development of immunochromatographic test system for the rapid detection of lipopolysaccharide antigen and cells of causative agent of bovine brucellosis]. / Byzova, N. A.; Zherdev, A. V.; Eskendirova, S. Z.; Baltin, K. K.; Unysheva, G. B.; Mukanov, K. K.; Ramankulov, E. M.; Dzantiev, B. B.

In: Prikladnaia biokhimiia i mikrobiologiia, Vol. 48, No. 6, 11.2012, p. 653-661.

Research output: Contribution to journalArticle

Byzova, N. A. ; Zherdev, A. V. ; Eskendirova, S. Z. ; Baltin, K. K. ; Unysheva, G. B. ; Mukanov, K. K. ; Ramankulov, E. M. ; Dzantiev, B. B. / [Development of immunochromatographic test system for the rapid detection of lipopolysaccharide antigen and cells of causative agent of bovine brucellosis]. In: Prikladnaia biokhimiia i mikrobiologiia. 2012 ; Vol. 48, No. 6. pp. 653-661.
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AU - Baltin, K. K.

AU - Unysheva, G. B.

AU - Mukanov, K. K.

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