Development of Spectral Imaging Cytometry

Ivan Vorobyev; Aigul Kussanova; Natasha Barteneva

doi:Spectral flow cytometry is a new technology that enables measurements of fluorescent spectra and light scattering properties in diverse cellular populations with high precision. Modern instruments allow simultaneous determination of up to 40+ fluorescent dyes with heavily overlapping emission spectra, discrimination of autofluorescent signals in the stained specimens, and detailed analysis of diverse autofluorescence of different cells-from mammalian to chlorophyll-containing cells like cyanobacteria. In this paper, we review the history, compare modern conventional and spectral flow cytometers, and discuss several applications of spectral flow cytometry

Development of Spectral Imaging Cytometry

Ivan Vorobyev, Aigul Kussanova, Natasha Barteneva

Nazarbayev University

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

4 Citations (Scopus)

Abstract

Spectral flow cytometry is a new technology that enables measurements of fluorescent spectra and light scattering properties in diverse cellular populations with high precision. Modern instruments allow simultaneous determination of up to 40+ fluorescent dyes with heavily overlapping emission spectra, discrimination of autofluorescent signals in the stained specimens, and detailed analysis of diverse autofluorescence of different cells-from mammalian to chlorophyll-containing cells like cyanobacteria. In this paper, we review the history, compare modern conventional and spectral flow cytometers, and discuss several applications of spectral flow cytometry

Original language	English
Title of host publication	Spectral and Imaging Cytometry
Place of Publication	Clifton, N.J.
Publisher	Humana Press Inc.
Pages	3-22
Number of pages	19
Volume	2635
DOIs	https://doi.org/Spectral flow cytometry is a new technology that enables measurements of fluorescent spectra and light scattering properties in diverse cellular populations with high precision. Modern instruments allow simultaneous determination of up to 40+ fluorescent dyes with heavily overlapping emission spectra, discrimination of autofluorescent signals in the stained specimens, and detailed analysis of diverse autofluorescence of different cells-from mammalian to chlorophyll-containing cells like cyanobacteria. In this paper, we review the history, compare modern conventional and spectral flow cytometers, and discuss several applications of spectral flow cytometry https://doi.org/10.1007/978-1-0716-3020-4_1
Publication status	Published - Apr 2023

Funding

Work was supported by the Ministry of Health of the Republic of Kazakhstan under the program-targeted funding of the Ageing and Healthy Lifespan research program (IRN: 51760/Ф-М Р-19) and AP08857554 (Ministry of Education and Science, Kazakhstan) to IAV. NSB was funded by CRP 16482715 and SSH2020028 grants from Nazarbayev University, and AP14872088 MES grant (Kazakhstan).

Keywords

spectral flow cytometry
flow cytometry
fluorescence spectra
Aurora cytometer
Sony spectral analyzer
autofluorescence
Spectral unmixing
Virtual filtering

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Vorobyev, I., Kussanova, A., & Barteneva, N. (2023). Development of Spectral Imaging Cytometry. In Spectral and Imaging Cytometry (Vol. 2635, pp. 3-22). Humana Press Inc.. https://doi.org/Spectral flow cytometry is a new technology that enables measurements of fluorescent spectra and light scattering properties in diverse cellular populations with high precision. Modern instruments allow simultaneous determination of up to 40+ fluorescent dyes with heavily overlapping emission spectra, discrimination of autofluorescent signals in the stained specimens, and detailed analysis of diverse autofluorescence of different cells-from mammalian to chlorophyll-containing cells like cyanobacteria. In this paper, we review the history, compare modern conventional and spectral flow cytometers, and discuss several applications of spectral flow cytometry, https://doi.org/10.1007/978-1-0716-3020-4_1

Vorobyev, I, Kussanova, A & Barteneva, N 2023, Development of Spectral Imaging Cytometry. in Spectral and Imaging Cytometry. vol. 2635, Humana Press Inc., Clifton, N.J., pp. 3-22. https://doi.org/Spectral flow cytometry is a new technology that enables measurements of fluorescent spectra and light scattering properties in diverse cellular populations with high precision. Modern instruments allow simultaneous determination of up to 40+ fluorescent dyes with heavily overlapping emission spectra, discrimination of autofluorescent signals in the stained specimens, and detailed analysis of diverse autofluorescence of different cells-from mammalian to chlorophyll-containing cells like cyanobacteria. In this paper, we review the history, compare modern conventional and spectral flow cytometers, and discuss several applications of spectral flow cytometry, https://doi.org/10.1007/978-1-0716-3020-4_1

Vorobyev I, Kussanova A, Barteneva N. Development of Spectral Imaging Cytometry. In Spectral and Imaging Cytometry. Vol. 2635. Clifton, N.J.: Humana Press Inc. 2023. p. 3-22 doi: Spectral flow cytometry is a new technology that enables measurements of fluorescent spectra and light scattering properties in diverse cellular populations with high precision. Modern instruments allow simultaneous determination of up to 40+ fluorescent dyes with heavily overlapping emission spectra, discrimination of autofluorescent signals in the stained specimens, and detailed analysis of diverse autofluorescence of different cells-from mammalian to chlorophyll-containing cells like cyanobacteria. In this paper, we review the history, compare modern conventional and spectral flow cytometers, and discuss several applications of spectral flow cytometry, 10.1007/978-1-0716-3020-4_1

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KW - Aurora cytometer

KW - Sony spectral analyzer

KW - autofluorescence

KW - Spectral unmixing

KW - Virtual filtering

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DO - Spectral flow cytometry is a new technology that enables measurements of fluorescent spectra and light scattering properties in diverse cellular populations with high precision. Modern instruments allow simultaneous determination of up to 40+ fluorescent dyes with heavily overlapping emission spectra, discrimination of autofluorescent signals in the stained specimens, and detailed analysis of diverse autofluorescence of different cells-from mammalian to chlorophyll-containing cells like cyanobacteria. In this paper, we review the history, compare modern conventional and spectral flow cytometers, and discuss several applications of spectral flow cytometry

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BT - Spectral and Imaging Cytometry

PB - Humana Press Inc.

CY - Clifton, N.J.

ER -

Development of Spectral Imaging Cytometry

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