Different autonomous myogenic cell populations revealed by ablation of Myf5-expressing cells during mouse embryogenesis

Nicole Gensch, Thilo Borchardt, Andre Schneider, Dieter Riethmacher, Thomas Braun

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

The development of myogenic cells is mainly determined by expression of two myogenic factors, Myf5 and Myod1 (MyoD), which genetically compensate for each other during embryogenesis. Here, we demonstrate by conditional cell ablation in mice that Myf5 determines a distinct myogenic cell population, which also contains some Myod1-positive cells. Ablation of this lineage uncovers the presence of a second autonomous myogenic lineage, which superseded Myf5-dependent myogenic cells and expressed Myod1. By contrast, ablation of myogenin-expressing cells erased virtually all differentiated muscle cells, indicating that some aspects of the myogenic program are shared by most skeletal muscle cells. We conclude that Myf5 and Myod1 define different cell lineages with distinct contributions to muscle precursor cells and differentiated myotubes. Individual myogenic cell lineages seem to substitute for each other within the developing embryo.

Original languageEnglish
Pages (from-to)1597-1604
Number of pages8
JournalDevelopment (Cambridge)
Volume135
Issue number9
DOIs
Publication statusPublished - May 2008
Externally publishedYes

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Embryonic Development
Population
Cell Lineage
Muscle Cells
Myogenin
Myoblasts
Skeletal Muscle Fibers
Skeletal Muscle
Embryonic Structures

Keywords

  • Cell ablation
  • MyfS
  • Myogenic cell lineages
  • Skeletal muscle

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Different autonomous myogenic cell populations revealed by ablation of Myf5-expressing cells during mouse embryogenesis. / Gensch, Nicole; Borchardt, Thilo; Schneider, Andre; Riethmacher, Dieter; Braun, Thomas.

In: Development (Cambridge), Vol. 135, No. 9, 05.2008, p. 1597-1604.

Research output: Contribution to journalArticle

Gensch, Nicole ; Borchardt, Thilo ; Schneider, Andre ; Riethmacher, Dieter ; Braun, Thomas. / Different autonomous myogenic cell populations revealed by ablation of Myf5-expressing cells during mouse embryogenesis. In: Development (Cambridge). 2008 ; Vol. 135, No. 9. pp. 1597-1604.
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