Differential stimulation of monocytic cells results in distinct populations of microparticles

M. Bernimoulin, E. K. Waters, M. Foy, B. M. Steele, M. Sullivan, H. Falet, M. T. Walsh, N. Barteneva, J. G. Geng, J. H. Hartwig, P. B. Maguire, D. D. Wagner

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Background: Microparticles (MPs), small vesicles shed from stimulated cells, permit cross-talk between cells within a particular environment. Their composition is thought to reflect their cell of origin, and differs according to whether they are produced by stimulation or by apoptosis. Whether MP properties vary according to stimulus is not yet known. Methods: We studied the characteristics of MPs produced from monocytic THP-1 cells upon stimulation with lipopolysaccharide or a soluble P-selectin chimera, using proteomics, flow cytometry, western blotting, and electron microscopy. Results: Utilizing a novel criterion of calcein-AM staining to define MPs, we found that MP populations were similar with respect to size, presence and organization of cytoskeleton, and expression of certain antigens. The MPs shared the same level of procoagulant activity. We found that MPs also have distinct characteristics, depending on stimuli. These include differences in phosphatidylserine expression and expression of proteins from specific subcellular locations such as the mitochondria, and of unique antigens such as leukocyte-associated immunoglobin-like-receptor (LAIR)-1, which was found only upon stimulation with the soluble P-selectin chimera. Conclusion: We found that the properties of MPs depend on the stimulus that produced them. This supports the concept that monocytic MPs differentially modulate thrombosis, inflammation and immune regulation according to stimulus.

Original languageEnglish
Pages (from-to)1019-1028
Number of pages10
JournalJournal of Thrombosis and Haemostasis
Volume7
Issue number6
DOIs
Publication statusPublished - 2009
Externally publishedYes

Fingerprint

P-Selectin
Population
Antigens
Phosphatidylserines
Cytoskeleton
Proteomics
Lipopolysaccharides
Electron Microscopy
Flow Cytometry
Mitochondria
Thrombosis
Leukocytes
Western Blotting
Apoptosis
Staining and Labeling
Inflammation
Proteins
calcein AM

Keywords

  • LPS
  • Microparticles
  • Monocytes
  • P-selectin
  • Phosphatidylserine
  • Proteomics

ASJC Scopus subject areas

  • Hematology

Cite this

Bernimoulin, M., Waters, E. K., Foy, M., Steele, B. M., Sullivan, M., Falet, H., ... Wagner, D. D. (2009). Differential stimulation of monocytic cells results in distinct populations of microparticles. Journal of Thrombosis and Haemostasis, 7(6), 1019-1028. https://doi.org/10.1111/j.1538-7836.2009.03434.x

Differential stimulation of monocytic cells results in distinct populations of microparticles. / Bernimoulin, M.; Waters, E. K.; Foy, M.; Steele, B. M.; Sullivan, M.; Falet, H.; Walsh, M. T.; Barteneva, N.; Geng, J. G.; Hartwig, J. H.; Maguire, P. B.; Wagner, D. D.

In: Journal of Thrombosis and Haemostasis, Vol. 7, No. 6, 2009, p. 1019-1028.

Research output: Contribution to journalArticle

Bernimoulin, M, Waters, EK, Foy, M, Steele, BM, Sullivan, M, Falet, H, Walsh, MT, Barteneva, N, Geng, JG, Hartwig, JH, Maguire, PB & Wagner, DD 2009, 'Differential stimulation of monocytic cells results in distinct populations of microparticles', Journal of Thrombosis and Haemostasis, vol. 7, no. 6, pp. 1019-1028. https://doi.org/10.1111/j.1538-7836.2009.03434.x
Bernimoulin, M. ; Waters, E. K. ; Foy, M. ; Steele, B. M. ; Sullivan, M. ; Falet, H. ; Walsh, M. T. ; Barteneva, N. ; Geng, J. G. ; Hartwig, J. H. ; Maguire, P. B. ; Wagner, D. D. / Differential stimulation of monocytic cells results in distinct populations of microparticles. In: Journal of Thrombosis and Haemostasis. 2009 ; Vol. 7, No. 6. pp. 1019-1028.
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