Dinitrophenyl (DNP) hapten sandwich staining (DHSS) procedure. A 10 year review of its principle reagents and applications

B. Jasani, N. D. Thomas, H. Navabi, D. M. Millar, G. R. Newman, J. Gee, E. D. Williams

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Over the past 10 years an immunoperoxidase method using dinitrophenyl (DNP) hapten-labelled primary or secondary probes has been devised. Its widely successful application in research and diagnostic work has depended upon the development of certain key reagents. These include a novel non-deleterious DNP labelling compound, a unique multivalent monoclonal bridge antibody, and an efficient DNP hapten substituted or anti-DNP linked marker enzyme. In this article the development of these reagents and various modifications of the basic technique are reviewed in conjunction with the special applications accruing from their use.

Original languageEnglish
Pages (from-to)193-198
Number of pages6
JournalJournal of Immunological Methods
Volume150
Issue number1-2
DOIs
Publication statusPublished - Jun 24 1992
Externally publishedYes

Fingerprint

Haptens
Dinitrobenzenes
Staining and Labeling
Monoclonal Antibodies
Enzymes
Research

Keywords

  • Dinitrophenyl
  • Dinitrophenyl localization system
  • Dinitrophenyl-hapten procedure
  • Immunoassay
  • Immunolocalization
  • Immunoperoxidase

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

Dinitrophenyl (DNP) hapten sandwich staining (DHSS) procedure. A 10 year review of its principle reagents and applications. / Jasani, B.; Thomas, N. D.; Navabi, H.; Millar, D. M.; Newman, G. R.; Gee, J.; Williams, E. D.

In: Journal of Immunological Methods, Vol. 150, No. 1-2, 24.06.1992, p. 193-198.

Research output: Contribution to journalArticle

Jasani, B. ; Thomas, N. D. ; Navabi, H. ; Millar, D. M. ; Newman, G. R. ; Gee, J. ; Williams, E. D. / Dinitrophenyl (DNP) hapten sandwich staining (DHSS) procedure. A 10 year review of its principle reagents and applications. In: Journal of Immunological Methods. 1992 ; Vol. 150, No. 1-2. pp. 193-198.
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