Abstract
Surface plasmon resonance (SPR) spectroscopy was applied for the first time to study online the association of the blood-brain barrier proteins ZO-1 (zona occludens protein 1) and occludin. The GuK motif of ZO-1 (amino acids 644-812) was identified as the sequence domain that bound to the cytosolic, C-terminal tail of occludin (amino acids 378-521). The nitric oxide (·NO) liberator SIN-1 (3-morpholinosydnonimine) reduced the binding affinity of ZO-1 to occludin, indicating a direct effect of ·NO and NO-related species on the tight junction proteins sealing the blood-brain barrier. We propose that SPR spectroscopy is a novel approach for investigating the interactions of tight junction proteins directly and continuously for pharmacological studies.
| Original language | English |
|---|---|
| Title of host publication | Blood-Spinal Cord and Brain Barriers in Health and Disease |
| Publisher | Elsevier |
| Pages | 11-17 |
| Number of pages | 7 |
| ISBN (Print) | 9780080528229, 9780126390117 |
| DOIs | |
| Publication status | Published - Nov 21 2003 |
| Externally published | Yes |
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Keywords
- NO, SIN-1
- Occludin
- Protein interaction
- SPR
- Tight junctions
- ZO-1
ASJC Scopus subject areas
- Medicine(all)
- Neuroscience(all)
Cite this
Direct Demonstration of Association of the Blood-Brain Barrier Proteins ZO-1 and Occludin Using Surface Plasmon Resonance Spectroscopy; Effect of SIN-1. / Schmidt, Anke; Utepbergenov, Darkhan I.; Krause, Gerd; Blasig, Ingolf F.
Blood-Spinal Cord and Brain Barriers in Health and Disease. Elsevier, 2003. p. 11-17.Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Direct Demonstration of Association of the Blood-Brain Barrier Proteins ZO-1 and Occludin Using Surface Plasmon Resonance Spectroscopy; Effect of SIN-1
AU - Schmidt, Anke
AU - Utepbergenov, Darkhan I.
AU - Krause, Gerd
AU - Blasig, Ingolf F.
PY - 2003/11/21
Y1 - 2003/11/21
N2 - Surface plasmon resonance (SPR) spectroscopy was applied for the first time to study online the association of the blood-brain barrier proteins ZO-1 (zona occludens protein 1) and occludin. The GuK motif of ZO-1 (amino acids 644-812) was identified as the sequence domain that bound to the cytosolic, C-terminal tail of occludin (amino acids 378-521). The nitric oxide (·NO) liberator SIN-1 (3-morpholinosydnonimine) reduced the binding affinity of ZO-1 to occludin, indicating a direct effect of ·NO and NO-related species on the tight junction proteins sealing the blood-brain barrier. We propose that SPR spectroscopy is a novel approach for investigating the interactions of tight junction proteins directly and continuously for pharmacological studies.
AB - Surface plasmon resonance (SPR) spectroscopy was applied for the first time to study online the association of the blood-brain barrier proteins ZO-1 (zona occludens protein 1) and occludin. The GuK motif of ZO-1 (amino acids 644-812) was identified as the sequence domain that bound to the cytosolic, C-terminal tail of occludin (amino acids 378-521). The nitric oxide (·NO) liberator SIN-1 (3-morpholinosydnonimine) reduced the binding affinity of ZO-1 to occludin, indicating a direct effect of ·NO and NO-related species on the tight junction proteins sealing the blood-brain barrier. We propose that SPR spectroscopy is a novel approach for investigating the interactions of tight junction proteins directly and continuously for pharmacological studies.
KW - NO, SIN-1
KW - Occludin
KW - Protein interaction
KW - SPR
KW - Tight junctions
KW - ZO-1
UR - http://www.scopus.com/inward/record.url?scp=84942873508&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84942873508&partnerID=8YFLogxK
U2 - 10.1016/B978-012639011-7/50006-1
DO - 10.1016/B978-012639011-7/50006-1
M3 - Chapter
AN - SCOPUS:84942873508
SN - 9780080528229
SN - 9780126390117
SP - 11
EP - 17
BT - Blood-Spinal Cord and Brain Barriers in Health and Disease
PB - Elsevier
ER -