Direct Demonstration of Association of the Blood-Brain Barrier Proteins ZO-1 and Occludin Using Surface Plasmon Resonance Spectroscopy; Effect of SIN-1

Anke Schmidt, Darkhan I. Utepbergenov, Gerd Krause, Ingolf F. Blasig

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citations (Scopus)

Abstract

Surface plasmon resonance (SPR) spectroscopy was applied for the first time to study online the association of the blood-brain barrier proteins ZO-1 (zona occludens protein 1) and occludin. The GuK motif of ZO-1 (amino acids 644-812) was identified as the sequence domain that bound to the cytosolic, C-terminal tail of occludin (amino acids 378-521). The nitric oxide (·NO) liberator SIN-1 (3-morpholinosydnonimine) reduced the binding affinity of ZO-1 to occludin, indicating a direct effect of ·NO and NO-related species on the tight junction proteins sealing the blood-brain barrier. We propose that SPR spectroscopy is a novel approach for investigating the interactions of tight junction proteins directly and continuously for pharmacological studies.

Original languageEnglish
Title of host publicationBlood-Spinal Cord and Brain Barriers in Health and Disease
PublisherElsevier
Pages11-17
Number of pages7
ISBN (Print)9780080528229, 9780126390117
DOIs
Publication statusPublished - Nov 21 2003
Externally publishedYes

Fingerprint

Zonula Occludens-1 Protein
Occludin
Surface Plasmon Resonance
Blood-Brain Barrier
Tight Junction Proteins
Spectrum Analysis
Nitric Oxide
Amino Acids
Proteins
Pharmacology

Keywords

  • NO, SIN-1
  • Occludin
  • Protein interaction
  • SPR
  • Tight junctions
  • ZO-1

ASJC Scopus subject areas

  • Medicine(all)
  • Neuroscience(all)

Cite this

Direct Demonstration of Association of the Blood-Brain Barrier Proteins ZO-1 and Occludin Using Surface Plasmon Resonance Spectroscopy; Effect of SIN-1. / Schmidt, Anke; Utepbergenov, Darkhan I.; Krause, Gerd; Blasig, Ingolf F.

Blood-Spinal Cord and Brain Barriers in Health and Disease. Elsevier, 2003. p. 11-17.

Research output: Chapter in Book/Report/Conference proceedingChapter

Schmidt, Anke ; Utepbergenov, Darkhan I. ; Krause, Gerd ; Blasig, Ingolf F. / Direct Demonstration of Association of the Blood-Brain Barrier Proteins ZO-1 and Occludin Using Surface Plasmon Resonance Spectroscopy; Effect of SIN-1. Blood-Spinal Cord and Brain Barriers in Health and Disease. Elsevier, 2003. pp. 11-17
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