Direct Demonstration of Association of the Blood-Brain Barrier Proteins ZO-1 and Occludin Using Surface Plasmon Resonance Spectroscopy; Effect of SIN-1

Anke Schmidt; Darkhan I. Utepbergenov; Gerd Krause; Ingolf F. Blasig

doi:10.1016/B978-012639011-7/50006-1

Direct Demonstration of Association of the Blood-Brain Barrier Proteins ZO-1 and Occludin Using Surface Plasmon Resonance Spectroscopy; Effect of SIN-1

Anke Schmidt, Darkhan I. Utepbergenov, Gerd Krause, Ingolf F. Blasig

Department of Chemistry

Research output: Chapter in Book/Report/Conference proceeding › Chapter

2 Citations (Scopus)

Abstract

Surface plasmon resonance (SPR) spectroscopy was applied for the first time to study online the association of the blood-brain barrier proteins ZO-1 (zona occludens protein 1) and occludin. The GuK motif of ZO-1 (amino acids 644-812) was identified as the sequence domain that bound to the cytosolic, C-terminal tail of occludin (amino acids 378-521). The nitric oxide (·NO) liberator SIN-1 (3-morpholinosydnonimine) reduced the binding affinity of ZO-1 to occludin, indicating a direct effect of ·NO and NO-related species on the tight junction proteins sealing the blood-brain barrier. We propose that SPR spectroscopy is a novel approach for investigating the interactions of tight junction proteins directly and continuously for pharmacological studies.

Original language	English
Title of host publication	Blood-Spinal Cord and Brain Barriers in Health and Disease
Publisher	Elsevier
Pages	11-17
Number of pages	7
ISBN (Electronic)	9780080528229
ISBN (Print)	9780126390117
DOIs	https://doi.org/10.1016/B978-012639011-7/50006-1
Publication status	Published - Jan 1 2004

Keywords

NO, SIN-1
Occludin
Protein interaction
SPR
Tight junctions
ZO-1

ASJC Scopus subject areas

Medicine(all)
Neuroscience(all)

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10.1016/B978-012639011-7/50006-1

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Direct Demonstration of Association of the Blood-Brain Barrier Proteins ZO-1 and Occludin Using Surface Plasmon Resonance Spectroscopy; Effect of SIN-1. / Schmidt, Anke; Utepbergenov, Darkhan I.; Krause, Gerd; Blasig, Ingolf F.

Blood-Spinal Cord and Brain Barriers in Health and Disease. Elsevier, 2004. p. 11-17.

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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abstract = "Surface plasmon resonance (SPR) spectroscopy was applied for the first time to study online the association of the blood-brain barrier proteins ZO-1 (zona occludens protein 1) and occludin. The GuK motif of ZO-1 (amino acids 644-812) was identified as the sequence domain that bound to the cytosolic, C-terminal tail of occludin (amino acids 378-521). The nitric oxide (·NO) liberator SIN-1 (3-morpholinosydnonimine) reduced the binding affinity of ZO-1 to occludin, indicating a direct effect of ·NO and NO-related species on the tight junction proteins sealing the blood-brain barrier. We propose that SPR spectroscopy is a novel approach for investigating the interactions of tight junction proteins directly and continuously for pharmacological studies.",

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