Direct Demonstration of Association of the Blood-Brain Barrier Proteins ZO-1 and Occludin Using Surface Plasmon Resonance Spectroscopy; Effect of SIN-1

Anke Schmidt; Darkhan I. Utepbergenov; Gerd Krause; Ingolf F. Blasig

doi:10.1016/B978-012639011-7/50006-1

Direct Demonstration of Association of the Blood-Brain Barrier Proteins ZO-1 and Occludin Using Surface Plasmon Resonance Spectroscopy; Effect of SIN-1

Anke Schmidt, Darkhan I. Utepbergenov, Gerd Krause, Ingolf F. Blasig

Department of Chemistry

Research output: Chapter in Book/Report/Conference proceeding › Chapter

3 Citations (Scopus)

Abstract

Surface plasmon resonance (SPR) spectroscopy was applied for the first time to study online the association of the blood-brain barrier proteins ZO-1 (zona occludens protein 1) and occludin. The GuK motif of ZO-1 (amino acids 644-812) was identified as the sequence domain that bound to the cytosolic, C-terminal tail of occludin (amino acids 378-521). The nitric oxide (·NO) liberator SIN-1 (3-morpholinosydnonimine) reduced the binding affinity of ZO-1 to occludin, indicating a direct effect of ·NO and NO-related species on the tight junction proteins sealing the blood-brain barrier. We propose that SPR spectroscopy is a novel approach for investigating the interactions of tight junction proteins directly and continuously for pharmacological studies.

Original language	English
Title of host publication	Blood-Spinal Cord and Brain Barriers in Health and Disease
Publisher	Elsevier
Pages	11-17
Number of pages	7
ISBN (Electronic)	9780080528229
ISBN (Print)	9780126390117
DOIs	https://doi.org/10.1016/B978-012639011-7/50006-1
Publication status	Published - 2004

Keywords

NO, SIN-1
Occludin
Protein interaction
SPR
Tight junctions
ZO-1

ASJC Scopus subject areas

Medicine(all)
Neuroscience(all)

Access to Document

10.1016/B978-012639011-7/50006-1

Cite this

Direct Demonstration of Association of the Blood-Brain Barrier Proteins ZO-1 and Occludin Using Surface Plasmon Resonance Spectroscopy; Effect of SIN-1. / Schmidt, Anke; Utepbergenov, Darkhan I.; Krause, Gerd; Blasig, Ingolf F.

Blood-Spinal Cord and Brain Barriers in Health and Disease. Elsevier, 2004. p. 11-17.

Research output: Chapter in Book/Report/Conference proceeding › Chapter

@inbook{82c909616af94cefa261787b788268f7,

title = "Direct Demonstration of Association of the Blood-Brain Barrier Proteins ZO-1 and Occludin Using Surface Plasmon Resonance Spectroscopy; Effect of SIN-1",

abstract = "Surface plasmon resonance (SPR) spectroscopy was applied for the first time to study online the association of the blood-brain barrier proteins ZO-1 (zona occludens protein 1) and occludin. The GuK motif of ZO-1 (amino acids 644-812) was identified as the sequence domain that bound to the cytosolic, C-terminal tail of occludin (amino acids 378-521). The nitric oxide (·NO) liberator SIN-1 (3-morpholinosydnonimine) reduced the binding affinity of ZO-1 to occludin, indicating a direct effect of ·NO and NO-related species on the tight junction proteins sealing the blood-brain barrier. We propose that SPR spectroscopy is a novel approach for investigating the interactions of tight junction proteins directly and continuously for pharmacological studies.",

keywords = "NO, SIN-1, Occludin, Protein interaction, SPR, Tight junctions, ZO-1",

author = "Anke Schmidt and Utepbergenov, {Darkhan I.} and Gerd Krause and Blasig, {Ingolf F.}",

note = "Funding Information: We thank Gislinde Hartmann for technical assistance. We appreciate the assistance of John Dickson in preparation of the manuscript. This work was supported by DFG BL308/6-1, DFG SFB 507 A2, and BMBF BEO 10015B/1466C. Publisher Copyright: {\textcopyright} 2004 Elsevier Inc. All rights reserved.",

year = "2004",

doi = "10.1016/B978-012639011-7/50006-1",

language = "English",

isbn = "9780126390117",

pages = "11--17",

booktitle = "Blood-Spinal Cord and Brain Barriers in Health and Disease",

publisher = "Elsevier",

address = "Netherlands",

}

TY - CHAP

T1 - Direct Demonstration of Association of the Blood-Brain Barrier Proteins ZO-1 and Occludin Using Surface Plasmon Resonance Spectroscopy; Effect of SIN-1

AU - Schmidt, Anke

AU - Utepbergenov, Darkhan I.

AU - Krause, Gerd

AU - Blasig, Ingolf F.

N1 - Funding Information: We thank Gislinde Hartmann for technical assistance. We appreciate the assistance of John Dickson in preparation of the manuscript. This work was supported by DFG BL308/6-1, DFG SFB 507 A2, and BMBF BEO 10015B/1466C. Publisher Copyright: © 2004 Elsevier Inc. All rights reserved.

PY - 2004

Y1 - 2004

N2 - Surface plasmon resonance (SPR) spectroscopy was applied for the first time to study online the association of the blood-brain barrier proteins ZO-1 (zona occludens protein 1) and occludin. The GuK motif of ZO-1 (amino acids 644-812) was identified as the sequence domain that bound to the cytosolic, C-terminal tail of occludin (amino acids 378-521). The nitric oxide (·NO) liberator SIN-1 (3-morpholinosydnonimine) reduced the binding affinity of ZO-1 to occludin, indicating a direct effect of ·NO and NO-related species on the tight junction proteins sealing the blood-brain barrier. We propose that SPR spectroscopy is a novel approach for investigating the interactions of tight junction proteins directly and continuously for pharmacological studies.

AB - Surface plasmon resonance (SPR) spectroscopy was applied for the first time to study online the association of the blood-brain barrier proteins ZO-1 (zona occludens protein 1) and occludin. The GuK motif of ZO-1 (amino acids 644-812) was identified as the sequence domain that bound to the cytosolic, C-terminal tail of occludin (amino acids 378-521). The nitric oxide (·NO) liberator SIN-1 (3-morpholinosydnonimine) reduced the binding affinity of ZO-1 to occludin, indicating a direct effect of ·NO and NO-related species on the tight junction proteins sealing the blood-brain barrier. We propose that SPR spectroscopy is a novel approach for investigating the interactions of tight junction proteins directly and continuously for pharmacological studies.

KW - NO, SIN-1

KW - Occludin

KW - Protein interaction

KW - SPR

KW - Tight junctions

KW - ZO-1

UR - http://www.scopus.com/inward/record.url?scp=84942873508&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84942873508&partnerID=8YFLogxK

U2 - 10.1016/B978-012639011-7/50006-1

DO - 10.1016/B978-012639011-7/50006-1

M3 - Chapter

AN - SCOPUS:84942873508

SN - 9780126390117

SP - 11

EP - 17

BT - Blood-Spinal Cord and Brain Barriers in Health and Disease

PB - Elsevier

ER -

Direct Demonstration of Association of the Blood-Brain Barrier Proteins ZO-1 and Occludin Using Surface Plasmon Resonance Spectroscopy; Effect of SIN-1

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this