Introduction. Cervical cancer is estimated to be the fourth most common cancer in women worldwide and the leading cause of cancer death in developing countries1. Kazakhstan shows a high incidence rate of cervical cancer in women of all ages that had risen significantly to 18.2 per 100,000 women with over 1,700 new cervical cancer cases diagnosed annually2-4. Approximately 99% of all cervical cancer cases have been linked to human papillomavirus infection1,2. The prevalence of HR-HPV infection varies among countries worldwide with average of 30% among female population aged <252. Human papillomavirus (HPV) represents a great burden for the health of Kazakhstani women3. However, little is known about HPV prevalence in Kazakhstan3,4. The aims of this study were to genotype HPV DNA in cervical swab samples to determine the prevalence of HPV genotypes around the country, and to identify the differences between regions. Methods. Cervical swab samples were collected from women attending gynaecological clinics at five Kazakhstan cities (Nur-Sultan, Almaty, Aktobe, Pavlodar, and Oskemen), representing different parts of the country (Center, South, West, North, and East). In total, 890 women aged between 18 and 70 participated in the study. Samples were genotyped using real time multiplex PCR with Amplisense HPV genotyping kit. Descriptive statistical analysis was conducted on the obtained results using STATA software. Results. Preliminary data of HPV testing showed that 42,1% women attending gynaecological clinics were positive for HPV infection. There was no statistical difference in HPV prevalence between the regions (p=0.468). Among HPV positive women, high-risk HPV type 16 was the most prevalent (Figure 1). The rest of HPV types had a prevalence of less than 6%. Around 65% of HPV infected women had only one HPV genotype and almost 16% of women were co-infected with 2 HPV genotypes. Less than 1 % of women were co-infected with either 5 or 6 genotypes. Conclusion. This study preliminary data reveals high prevalence of HPV infection among women attending gynaecological clinics in Kazakhstan. The further large-scale investigation will help to identify more precise prevalence of HR-HPV infection. Together with strengthening the knowledge about HPV prevalence in Kazakhstan, the study final results can contribute to health policy development and decision-making about HPV vaccination and cervical cancer prevention. References. 1Chan CK, Aimagambetova G, Ukybassova T, Kongrtay K, Azizan A. Human Papillomavirus Infection and Cervical Cancer: Epidemiology, Screening, and Vaccination-Review of Current Perspectives. J Oncol. 2019 Oct 10;2019:3257939. 2 Bruni L, Albero G, Serrano B, Mena M, Gómez D, Muñoz J, Bosch FX, de Sanjosé S. ICO/IARC Information Centre on HPV and Cancer (HPV Information Centre). Human Papillomavirus and Related Diseases in the World. 3Aimagambetova G, et al. Epidemiology of HPV Infection and HPV-Related Cancers in Kazakhstan: a Review. Asian Pac J Cancer Prev. 2018 May 26;19(5):1175-1180. 4Aimagambetova G, Chan CK, Ukybassova T, et al. Cervical cancer screening and prevention in Kazakhstan and Central Asia. J Med Screen. 2020;969141320902482.
|Title of host publication||DISTRIBUTION OF HIGH-RISK HUMAN PAPILLOMAVIRUS GENOTYPES AMONG KAZAKHSTANI WOMEN|
|Publication status||Published - Nov 19 2020|