A two-step method of labelling DNA through aliphatic amino groups with various reporter molecules has been developed. The method is based on reaction of cytosine-residues of polynucleotide chain with 4-aminooxybutylamine; the latter's synthesis is described. DNA modified on this way with fluorescein isothiocyanate functions a probe in molecular hybridisation. The sensitivity of this method for fluorochrome-labelled DNA probes in the dot hybridisation procedure is about 200 pg per mm2.
|Translated title of the contribution
|DNA carrying aliphatic amino groups and the use of its fluorescent derivative as a probe in molecular hybridization
|Number of pages
|Published - Aug 1 1987
ASJC Scopus subject areas
- General Medicine