Izuchenie DNK, nesushcheǐ alifaticheskie aminogruppy, i ispol'zovanie ee fluorestsentnogo proizvodnogo v kachestve zonda pri molekuliarnoǐ gibridizatsii.

Translated title of the contribution: DNA carrying aliphatic amino groups and the use of its fluorescent derivative as a probe in molecular hybridization

V. A. Adarichev, G. M. Dymshits, S. M. Kalachikov, P. I. Pozdniakov, R. I. Salganik

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

A two-step method of labelling DNA through aliphatic amino groups with various reporter molecules has been developed. The method is based on reaction of cytosine-residues of polynucleotide chain with 4-aminooxybutylamine; the latter's synthesis is described. DNA modified on this way with fluorescein isothiocyanate functions a probe in molecular hybridisation. The sensitivity of this method for fluorochrome-labelled DNA probes in the dot hybridisation procedure is about 200 pg per mm2.

Translated title of the contributionDNA carrying aliphatic amino groups and the use of its fluorescent derivative as a probe in molecular hybridization
Original languageRussian
Pages (from-to)1066-1069
Number of pages4
JournalBioorganicheskaia khimiia
Volume13
Issue number8
Publication statusPublished - Aug 1 1987

ASJC Scopus subject areas

  • Medicine(all)

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