Izuchenie DNK, nesushcheǐ alifaticheskie aminogruppy, i ispol'zovanie ee fluorestsentnogo proizvodnogo v kachestve zonda pri molekuliarnoǐ gibridizatsii.

Translated title of the contribution: DNA carrying aliphatic amino groups and the use of its fluorescent derivative as a probe in molecular hybridization

V. A. Adarichev, G. M. Dymshits, S. M. Kalachikov, P. I. Pozdniakov, R. I. Salganik

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Abstract

A two-step method of labelling DNA through aliphatic amino groups with various reporter molecules has been developed. The method is based on reaction of cytosine-residues of polynucleotide chain with 4-aminooxybutylamine; the latter's synthesis is described. DNA modified on this way with fluorescein isothiocyanate functions a probe in molecular hybridisation. The sensitivity of this method for fluorochrome-labelled DNA probes in the dot hybridisation procedure is about 200 pg per mm2.

Original languageRussian
Pages (from-to)1066-1069
Number of pages4
JournalBioorganicheskaia khimiia
Volume13
Issue number8
Publication statusPublished - Aug 1 1987

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ASJC Scopus subject areas

  • Medicine(all)

Cite this

Adarichev, V. A., Dymshits, G. M., Kalachikov, S. M., Pozdniakov, P. I., & Salganik, R. I. (1987). Izuchenie DNK, nesushcheǐ alifaticheskie aminogruppy, i ispol'zovanie ee fluorestsentnogo proizvodnogo v kachestve zonda pri molekuliarnoǐ gibridizatsii. Bioorganicheskaia khimiia, 13(8), 1066-1069.