Izuchenie DNK, nesushcheǐ alifaticheskie aminogruppy, i ispol'zovanie ee fluorestsentnogo proizvodnogo v kachestve zonda pri molekuliarnoǐ gibridizatsii.

V. A. Adarichev; G. M. Dymshits; S. M. Kalachikov; P. I. Pozdniakov; R. I. Salganik

Izuchenie DNK, nesushcheǐ alifaticheskie aminogruppy, i ispol'zovanie ee fluorestsentnogo proizvodnogo v kachestve zonda pri molekuliarnoǐ gibridizatsii.

Translated title of the contribution: DNA carrying aliphatic amino groups and the use of its fluorescent derivative as a probe in molecular hybridization

V. A. Adarichev, G. M. Dymshits, S. M. Kalachikov, P. I. Pozdniakov, R. I. Salganik

Private Institution National Laboratory Astana

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

A two-step method of labelling DNA through aliphatic amino groups with various reporter molecules has been developed. The method is based on reaction of cytosine-residues of polynucleotide chain with 4-aminooxybutylamine; the latter's synthesis is described. DNA modified on this way with fluorescein isothiocyanate functions a probe in molecular hybridisation. The sensitivity of this method for fluorochrome-labelled DNA probes in the dot hybridisation procedure is about 200 pg per mm2.

Translated title of the contribution	DNA carrying aliphatic amino groups and the use of its fluorescent derivative as a probe in molecular hybridization
Original language	Russian
Pages (from-to)	1066-1069
Number of pages	4
Journal	Bioorganicheskaia khimiia
Volume	13
Issue number	8
Publication status	Published - Aug 1987

ASJC Scopus subject areas

General Medicine

Cite this

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title = "Izuchenie DNK, nesushcheǐ alifaticheskie aminogruppy, i ispol'zovanie ee fluorestsentnogo proizvodnogo v kachestve zonda pri molekuliarnoǐ gibridizatsii.",

abstract = "A two-step method of labelling DNA through aliphatic amino groups with various reporter molecules has been developed. The method is based on reaction of cytosine-residues of polynucleotide chain with 4-aminooxybutylamine; the latter's synthesis is described. DNA modified on this way with fluorescein isothiocyanate functions a probe in molecular hybridisation. The sensitivity of this method for fluorochrome-labelled DNA probes in the dot hybridisation procedure is about 200 pg per mm2.",

author = "Adarichev, {V. A.} and Dymshits, {G. M.} and Kalachikov, {S. M.} and Pozdniakov, {P. I.} and Salganik, {R. I.}",

year = "1987",

month = aug,

language = "Russian",

volume = "13",

pages = "1066--1069",

journal = "Bioorganicheskaia khimiia",

issn = "0132-3423",

publisher = "Izdatel'stvo Nauka",

number = "8",

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TY - JOUR

T1 - Izuchenie DNK, nesushcheǐ alifaticheskie aminogruppy, i ispol'zovanie ee fluorestsentnogo proizvodnogo v kachestve zonda pri molekuliarnoǐ gibridizatsii.

AU - Adarichev, V. A.

AU - Dymshits, G. M.

AU - Kalachikov, S. M.

AU - Pozdniakov, P. I.

AU - Salganik, R. I.

PY - 1987/8

Y1 - 1987/8

N2 - A two-step method of labelling DNA through aliphatic amino groups with various reporter molecules has been developed. The method is based on reaction of cytosine-residues of polynucleotide chain with 4-aminooxybutylamine; the latter's synthesis is described. DNA modified on this way with fluorescein isothiocyanate functions a probe in molecular hybridisation. The sensitivity of this method for fluorochrome-labelled DNA probes in the dot hybridisation procedure is about 200 pg per mm2.

AB - A two-step method of labelling DNA through aliphatic amino groups with various reporter molecules has been developed. The method is based on reaction of cytosine-residues of polynucleotide chain with 4-aminooxybutylamine; the latter's synthesis is described. DNA modified on this way with fluorescein isothiocyanate functions a probe in molecular hybridisation. The sensitivity of this method for fluorochrome-labelled DNA probes in the dot hybridisation procedure is about 200 pg per mm2.

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UR - http://www.scopus.com/inward/citedby.url?scp=0023392272&partnerID=8YFLogxK

M3 - Article

C2 - 3675649

AN - SCOPUS:0023392272

SN - 0132-3423

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SP - 1066

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JO - Bioorganicheskaia khimiia

JF - Bioorganicheskaia khimiia

IS - 8

ER -

Izuchenie DNK, nesushcheǐ alifaticheskie aminogruppy, i ispol'zovanie ee fluorestsentnogo proizvodnogo v kachestve zonda pri molekuliarnoǐ gibridizatsii.

Abstract

ASJC Scopus subject areas

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