A two-step method of labelling DNA through aliphatic amino groups with various reporter molecules has been developed. The method is based on reaction of cytosine-residues of polynucleotide chain with 4-aminooxybutylamine; the latter's synthesis is described. DNA modified on this way with fluorescein isothiocyanate functions a probe in molecular hybridisation. The sensitivity of this method for fluorochrome-labelled DNA probes in the dot hybridisation procedure is about 200 pg per mm2.
|Translated title of the contribution||DNA carrying aliphatic amino groups and the use of its fluorescent derivative as a probe in molecular hybridization|
|Number of pages||4|
|Publication status||Published - Aug 1 1987|
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