DNA modification by 4-aminooxybutylamine for preparation of highly sensitive hybridization probes. Localization of human chorionic somatomammotropin hormone gene

V. A. Adarichev, N. V. Vorobyeva, A. S. Grafodatsky, G. M. Dymshits, O. V. Dablina

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

A new method for preparation of highly sensitive nonradioactive probes for dot, dot-blot and in situ hybridization was developed. The method is based on chemical modification (transamination) of cytosine residues with 4-aminooxybutylamine following by coupling biotin or fluorescein to aliphatic aminogroups introduced into DNA. Such a probe have been used for detection of gene encoding chorionic somatomammotropin hormone (hCS) in genomic blot hybridization. The gene hCS was mapped using isotopic and nonisotopic in situ hybridization on human chromosome 17.

Original languageEnglish
Pages (from-to)538-545
Number of pages8
JournalMolekulyarnaya Biologiya
Volume29
Issue number3
Publication statusPublished - Dec 1 1995

ASJC Scopus subject areas

  • Medicine(all)

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