Abstract
A new method for preparation of highly sensitive nonradioactive probes for dot, dot-blot and in situ hybridization was developed. The method is based on chemical modification (transamination) of cytosine residues with 4-aminooxybutylamine following by coupling biotin or fluorescein to aliphatic aminogroups introduced into DNA. Such a probe have been used for detection of gene encoding chorionic somatomammotropin hormone (hCS) in genomic blot hybridization. The gene hCS was mapped using isotopic and nonisotopic in situ hybridization on human chromosome 17.
| Original language | English |
|---|---|
| Pages (from-to) | 538-545 |
| Number of pages | 8 |
| Journal | Molekulyarnaya Biologiya |
| Volume | 29 |
| Issue number | 3 |
| Publication status | Published - 1995 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Molecular Biology
Cite this
DNA modification by 4-aminooxybutylamine for preparation of highly sensitive hybridization probes. Localization of human chorionic somatomammotropin hormone gene. / Adarichev, V. A.; Vorobyeva, N. V.; Grafodatsky, A. S.; Dymshits, G. M.; Dablina, O. V.
In: Molekulyarnaya Biologiya, Vol. 29, No. 3, 1995, p. 538-545.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - DNA modification by 4-aminooxybutylamine for preparation of highly sensitive hybridization probes. Localization of human chorionic somatomammotropin hormone gene
AU - Adarichev, V. A.
AU - Vorobyeva, N. V.
AU - Grafodatsky, A. S.
AU - Dymshits, G. M.
AU - Dablina, O. V.
PY - 1995
Y1 - 1995
N2 - A new method for preparation of highly sensitive nonradioactive probes for dot, dot-blot and in situ hybridization was developed. The method is based on chemical modification (transamination) of cytosine residues with 4-aminooxybutylamine following by coupling biotin or fluorescein to aliphatic aminogroups introduced into DNA. Such a probe have been used for detection of gene encoding chorionic somatomammotropin hormone (hCS) in genomic blot hybridization. The gene hCS was mapped using isotopic and nonisotopic in situ hybridization on human chromosome 17.
AB - A new method for preparation of highly sensitive nonradioactive probes for dot, dot-blot and in situ hybridization was developed. The method is based on chemical modification (transamination) of cytosine residues with 4-aminooxybutylamine following by coupling biotin or fluorescein to aliphatic aminogroups introduced into DNA. Such a probe have been used for detection of gene encoding chorionic somatomammotropin hormone (hCS) in genomic blot hybridization. The gene hCS was mapped using isotopic and nonisotopic in situ hybridization on human chromosome 17.
UR - http://www.scopus.com/inward/record.url?scp=0029295973&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029295973&partnerID=8YFLogxK
M3 - Article
VL - 29
SP - 538
EP - 545
JO - Molekulyarnaya Biologiya
JF - Molekulyarnaya Biologiya
SN - 0026-8984
IS - 3
ER -