Downregulation of E-cadherin in the reparative Epithelium of the human gastrointestinal tract

Andrew M. Hanby, Rebecca Chinery, Richard Poulsom, Raymond J. Playford, Massimo Pignatelli

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

E-cadherin, an epithelial adhesion molecule, is critical for the maintenance of cell polarity and differentiation. We studied the distribution of E-cadherin in normal gut and in enteric ulceration to test the hypothesis that the motility of regenerative epithelium over ulcers is associated with a decrease in E-cadherin expression. Sections of normal stomach, small intestine, and colon were examined for E-cadherin distribution using the antibody HECD-1 and compared with the pattern seen in peptic ulceration and Crohn's disease. A subset was examined by in situ hybridization using 35S radiolabeled E-cadherin riboprobes. A wounding system employing the HT-29 cell line was used as an in vitro model of early healing. In the normal gut uniform strong basolateral staining was seen. Areas of ulceration showed a patchy reduction in membrane localized E-cadherin in regenerating epithelium, even though E-cadherin mRNA was demonstrable in this population. In wounded confluent HT29 layers, migrating cells also showed reduced E-cadherin immunostaining. These data support the notion that the motility of restitutive epithelial cells may relate to altered patterns of E-cadherin and that this may play an important role in the reconstitution of epithelial integrity after mucosal injury.

Original languageEnglish
Pages (from-to)723-729
Number of pages7
JournalAmerican Journal of Pathology
Volume148
Issue number3
Publication statusPublished - Mar 1996
Externally publishedYes

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Cadherins
Gastrointestinal Tract
Down-Regulation
Epithelium
HT29 Cells
Cell Polarity
Crohn Disease
Small Intestine
Ulcer
In Situ Hybridization
Cell Differentiation
Digestion
Stomach
Colon
Epithelial Cells
Maintenance
Staining and Labeling
Cell Line
Messenger RNA
Membranes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Downregulation of E-cadherin in the reparative Epithelium of the human gastrointestinal tract. / Hanby, Andrew M.; Chinery, Rebecca; Poulsom, Richard; Playford, Raymond J.; Pignatelli, Massimo.

In: American Journal of Pathology, Vol. 148, No. 3, 03.1996, p. 723-729.

Research output: Contribution to journalArticle

Hanby, Andrew M. ; Chinery, Rebecca ; Poulsom, Richard ; Playford, Raymond J. ; Pignatelli, Massimo. / Downregulation of E-cadherin in the reparative Epithelium of the human gastrointestinal tract. In: American Journal of Pathology. 1996 ; Vol. 148, No. 3. pp. 723-729.
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