Effect of electron transfer inhibitors on superoxide generation in the cytochrome bc1 site of the mitochondrial respiratory chain

Marina Ksenzenko, Alexander A. Konstantinov, Gennady B. Khomutov, Alexander N. Tikhonov, Enno K. Ruuge

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

Antimycin, 2-nonyl-4-hydroxyquinoline N-oxide and funiculosin induce O·- 2 generation by submitochondrial particles oxidizing succinate, whereas KCN, mucidin, myxothiazol or 2,3-dimercaptopropanol inhibit O·- 2 generation. Thenoyltrifluoroacetone does not induce superoxide production by itself but slightly stimulates the reaction initiated by antimycin. The results indicate that auto-oxidation of unstable ubisemiquinone formed in centre o of the Q-cycle generates most of the O·- 2 radicals in the cytochrome bc1-site of the mitochondrial respiratory chain.

Original languageEnglish
Pages (from-to)19-24
Number of pages6
JournalFEBS Letters
Volume155
Issue number1
DOIs
Publication statusPublished - May 2 1983
Externally publishedYes

Fingerprint

coenzyme Q10
Electron Transport Complex III
Electron Transport
Superoxides
Thenoyltrifluoroacetone
Submitochondrial Particles
Electrons
Dimercaprol
Succinic Acid
Oxidation
antimycin
mucidin
nonyl-4-hydroxyquinoline-N-oxide
myxothiazol
2-nonyl-4-hydroxyquinoline

Keywords

  • Cytochrome bc site
  • Electron-transfer inhibitor
  • Q cycle, center o
  • Respiratory chain, of mitochondria
  • Superoxide generation
  • Ubisemiquinone auto-oxidation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Effect of electron transfer inhibitors on superoxide generation in the cytochrome bc1 site of the mitochondrial respiratory chain. / Ksenzenko, Marina; Konstantinov, Alexander A.; Khomutov, Gennady B.; Tikhonov, Alexander N.; Ruuge, Enno K.

In: FEBS Letters, Vol. 155, No. 1, 02.05.1983, p. 19-24.

Research output: Contribution to journalArticle

Ksenzenko, Marina ; Konstantinov, Alexander A. ; Khomutov, Gennady B. ; Tikhonov, Alexander N. ; Ruuge, Enno K. / Effect of electron transfer inhibitors on superoxide generation in the cytochrome bc1 site of the mitochondrial respiratory chain. In: FEBS Letters. 1983 ; Vol. 155, No. 1. pp. 19-24.
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AU - Ruuge, Enno K.

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