Effect of electron transfer inhibitors on superoxide generation in the cytochrome bc1 site of the mitochondrial respiratory chain

Marina Ksenzenko, Alexander A. Konstantinov, Gennady B. Khomutov, Alexander N. Tikhonov, Enno K. Ruuge

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

Antimycin, 2-nonyl-4-hydroxyquinoline N-oxide and funiculosin induce O·-2 generation by submitochondrial particles oxidizing succinate, whereas KCN, mucidin, myxothiazol or 2,3-dimercaptopropanol inhibit O·-2 generation. Thenoyltrifluoroacetone does not induce superoxide production by itself but slightly stimulates the reaction initiated by antimycin. The results indicate that auto-oxidation of unstable ubisemiquinone formed in centre o of the Q-cycle generates most of the O·-2 radicals in the cytochrome bc1-site of the mitochondrial respiratory chain.

Original languageEnglish
Pages (from-to)19-24
Number of pages6
JournalFEBS Letters
Volume155
Issue number1
DOIs
Publication statusPublished - May 2 1983
Externally publishedYes

Keywords

  • Cytochrome bc site
  • Electron-transfer inhibitor
  • Q cycle, center o
  • Respiratory chain, of mitochondria
  • Superoxide generation
  • Ubisemiquinone auto-oxidation

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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