Abstract
Background: Numerous synthetic drugs have been recommended as a remedy for diabetes, but their role in hypoglycemic effects are diverse. The side effects associated with these drugs due to their extended use led scientists to find unconventional medicines with no or little side effects. Aim: This study was aimed at assessment of in vitro antidiabetic activities of methanolic extract of Litsea lancifolia leaves by using 3T3L1 cell line. Materials and Methods: The cytotoxic effect of the leaf extract was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The glucose uptake-inducing capabilities and its correlation with glucose transporter 4 (GLUT4) translocation were measured by flow cytometry in 3T3L1 cells. In addition, the inhibitory effect of L. lancifolia leaf extract on α-amylase activity and α-glucosidase activity was determined by colorimetric methods. Results: Different concentrations of L. lancifolia leaf extract did not show any toxicity on 3T3L1 cells, after the treatment for 24h. On stimulation with leaf extract, 60.22% and 86.26% of 3T3L1 cells showed glucose uptake and GLUT4 expression, respectively. The colorimetric assays showed that the methanolic leaf extract of L. lancifolia has a significant inhibitory effect on the activity of α-amylase enzyme and α-glucosidase enzyme with inhibitory concentration (IC50) value of 248.65 μg/mL and 229.61 μg/mL, respectively. Conclusion: On the basis of the results of this study, it is evident that L. lancifolia leaf extract showed promising anti-diabetic effect when compared to the standard drugs metformin and acarbose and was nontoxic to 3T3L1 cells. Thus, it can be further investigated to recommend as a possible alternative treatment in antidiabetic applications.
| Original language | English |
|---|---|
| Pages (from-to) | 240-247 |
| Number of pages | 8 |
| Journal | Journal of Pharmacy and Bioallied Sciences |
| Volume | 11 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jul 1 2019 |
| Externally published | Yes |
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Keywords
- 3T3L1 cell line
- Antidiabetic activity
- glucose transporter 4 expression studies
- glucosidase inhibition
- Litsea lancifolia
ASJC Scopus subject areas
- Bioengineering
- Biochemistry, Genetics and Molecular Biology(all)
- Pharmacology, Toxicology and Pharmaceutics(all)
Cite this
Effect of Litsea lancifolia leaf extract on glucose transporter 4 translocation and glucose uptake in 3T3L1 cell line. / Alsawalha, Murad; Al-Subaie, Abeer Mohammed; Al-Jindan, Reem Yousuf; Bolla, Srinivasa Rao; Balakrishna, Janardhana Papayya; Ravi, Padma Kanchi; Gollapalli, Shiva Shankar Reddy; Veeraraghavan, Vishnu Priya; Pillai, Aruthra Arumugam; Joseph, Joel Palpath; Mohan, Surapaneni Krishna.
In: Journal of Pharmacy and Bioallied Sciences, Vol. 11, No. 3, 01.07.2019, p. 240-247.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of Litsea lancifolia leaf extract on glucose transporter 4 translocation and glucose uptake in 3T3L1 cell line
AU - Alsawalha, Murad
AU - Al-Subaie, Abeer Mohammed
AU - Al-Jindan, Reem Yousuf
AU - Bolla, Srinivasa Rao
AU - Balakrishna, Janardhana Papayya
AU - Ravi, Padma Kanchi
AU - Gollapalli, Shiva Shankar Reddy
AU - Veeraraghavan, Vishnu Priya
AU - Pillai, Aruthra Arumugam
AU - Joseph, Joel Palpath
AU - Mohan, Surapaneni Krishna
PY - 2019/7/1
Y1 - 2019/7/1
N2 - Background: Numerous synthetic drugs have been recommended as a remedy for diabetes, but their role in hypoglycemic effects are diverse. The side effects associated with these drugs due to their extended use led scientists to find unconventional medicines with no or little side effects. Aim: This study was aimed at assessment of in vitro antidiabetic activities of methanolic extract of Litsea lancifolia leaves by using 3T3L1 cell line. Materials and Methods: The cytotoxic effect of the leaf extract was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The glucose uptake-inducing capabilities and its correlation with glucose transporter 4 (GLUT4) translocation were measured by flow cytometry in 3T3L1 cells. In addition, the inhibitory effect of L. lancifolia leaf extract on α-amylase activity and α-glucosidase activity was determined by colorimetric methods. Results: Different concentrations of L. lancifolia leaf extract did not show any toxicity on 3T3L1 cells, after the treatment for 24h. On stimulation with leaf extract, 60.22% and 86.26% of 3T3L1 cells showed glucose uptake and GLUT4 expression, respectively. The colorimetric assays showed that the methanolic leaf extract of L. lancifolia has a significant inhibitory effect on the activity of α-amylase enzyme and α-glucosidase enzyme with inhibitory concentration (IC50) value of 248.65 μg/mL and 229.61 μg/mL, respectively. Conclusion: On the basis of the results of this study, it is evident that L. lancifolia leaf extract showed promising anti-diabetic effect when compared to the standard drugs metformin and acarbose and was nontoxic to 3T3L1 cells. Thus, it can be further investigated to recommend as a possible alternative treatment in antidiabetic applications.
AB - Background: Numerous synthetic drugs have been recommended as a remedy for diabetes, but their role in hypoglycemic effects are diverse. The side effects associated with these drugs due to their extended use led scientists to find unconventional medicines with no or little side effects. Aim: This study was aimed at assessment of in vitro antidiabetic activities of methanolic extract of Litsea lancifolia leaves by using 3T3L1 cell line. Materials and Methods: The cytotoxic effect of the leaf extract was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The glucose uptake-inducing capabilities and its correlation with glucose transporter 4 (GLUT4) translocation were measured by flow cytometry in 3T3L1 cells. In addition, the inhibitory effect of L. lancifolia leaf extract on α-amylase activity and α-glucosidase activity was determined by colorimetric methods. Results: Different concentrations of L. lancifolia leaf extract did not show any toxicity on 3T3L1 cells, after the treatment for 24h. On stimulation with leaf extract, 60.22% and 86.26% of 3T3L1 cells showed glucose uptake and GLUT4 expression, respectively. The colorimetric assays showed that the methanolic leaf extract of L. lancifolia has a significant inhibitory effect on the activity of α-amylase enzyme and α-glucosidase enzyme with inhibitory concentration (IC50) value of 248.65 μg/mL and 229.61 μg/mL, respectively. Conclusion: On the basis of the results of this study, it is evident that L. lancifolia leaf extract showed promising anti-diabetic effect when compared to the standard drugs metformin and acarbose and was nontoxic to 3T3L1 cells. Thus, it can be further investigated to recommend as a possible alternative treatment in antidiabetic applications.
KW - 3T3L1 cell line
KW - Antidiabetic activity
KW - glucose transporter 4 expression studies
KW - glucosidase inhibition
KW - Litsea lancifolia
UR - http://www.scopus.com/inward/record.url?scp=85068857110&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85068857110&partnerID=8YFLogxK
U2 - 10.4103/jpbs.JPBS_53_19
DO - 10.4103/jpbs.JPBS_53_19
M3 - Article
AN - SCOPUS:85068857110
VL - 11
SP - 240
EP - 247
JO - Journal of Pharmacy and Bioallied Sciences
JF - Journal of Pharmacy and Bioallied Sciences
SN - 0976-4879
IS - 3
ER -