TY - JOUR
T1 - Effect of Mare’s milk prebiotic supplementation on the gut microbiome and the immune system following antibiotic therapy
AU - Nurgaziyev, Madiyar
AU - Aitenov, Yermek
AU - Khassenbekova, Zhanagul
AU - Akpanova, Saniya
AU - Rysbekov, Kairat
AU - Kozhakhmetov, Samat
AU - Nurgozhina, Ayaulym
AU - Sergazy, Shynggys
AU - Chulenbayeva, Laura
AU - Ospanova, Zhanna
AU - Tuyakova, Altynai
AU - Mukhambetganov, Nurislam
AU - Sattybayeva, Rashida
AU - Urazova, Saltanat
AU - Galymgozhina, Nazym
AU - Zhumadilova, Anara
AU - Gulyaev, Alexandr
AU - Kushugulova, Almagul
N1 - Funding Information:
This study was supported by scientific grants (AP05135273, AP05134659, and AP09058099) from the Ministry of Education and Science of Kazakhstan.
Publisher Copyright:
© 2020, Society for Indonesian Biodiversity. All rights reserved.
PY - 2020/11
Y1 - 2020/11
N2 - Antibiotic treatment can severely affect the gut microbiome for short-term and long-term consequences. Probiotic and prebiotic supplements are widely prescribed to modulate the composition and function of the human gut microbiome. The current study aims to determine the impacts of mare’s milk prebiotics on the diversity of gut bacterial communities and the local immune system when administered during and after a course of antibiotic therapy. Six children aged 4 to 5 years diagnosed with bilateral bronchopneumonia were prescribed cephalosporin (cefuroxime) antibiotics. During the 60 days of the study, three children consumed mare’s milk prebiotics, while the other three did not. Fecal samples were collected daily during antibiotic therapy and every five days after the last day of antibiotic treatment. Total DNA was isolated, and the taxonomic composition of the gut microbiome was analyzed by sequencing the 16S rRNA gene (V1-V3 region). The MULTIPLEX MAP platform was used to evaluate the local immune status. The relative abundance of 11 genera was reduced and did not recover until the last day of the study. The abundance of Bacteroides was not significantly altered in either group. Christensenella, Rothia, Abiotrophia, Acinetobacter, Anaerotruncus, Holdemania, and Turicibacter numbers were significantly increased on day five and remained at the same level during the study period. Cephalosporin administration also reduced the levels of pro-inflammatory and anti-inflammatory cytokines/chemokines (MIP1α, TNFα, GMCSF, GCSF, sCD40L, FGF2, TGFα, IL1α, and IP10).
AB - Antibiotic treatment can severely affect the gut microbiome for short-term and long-term consequences. Probiotic and prebiotic supplements are widely prescribed to modulate the composition and function of the human gut microbiome. The current study aims to determine the impacts of mare’s milk prebiotics on the diversity of gut bacterial communities and the local immune system when administered during and after a course of antibiotic therapy. Six children aged 4 to 5 years diagnosed with bilateral bronchopneumonia were prescribed cephalosporin (cefuroxime) antibiotics. During the 60 days of the study, three children consumed mare’s milk prebiotics, while the other three did not. Fecal samples were collected daily during antibiotic therapy and every five days after the last day of antibiotic treatment. Total DNA was isolated, and the taxonomic composition of the gut microbiome was analyzed by sequencing the 16S rRNA gene (V1-V3 region). The MULTIPLEX MAP platform was used to evaluate the local immune status. The relative abundance of 11 genera was reduced and did not recover until the last day of the study. The abundance of Bacteroides was not significantly altered in either group. Christensenella, Rothia, Abiotrophia, Acinetobacter, Anaerotruncus, Holdemania, and Turicibacter numbers were significantly increased on day five and remained at the same level during the study period. Cephalosporin administration also reduced the levels of pro-inflammatory and anti-inflammatory cytokines/chemokines (MIP1α, TNFα, GMCSF, GCSF, sCD40L, FGF2, TGFα, IL1α, and IP10).
KW - Antibiotic treatment
KW - Immune system
KW - Mare’s milk
KW - Microbiome
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U2 - 10.13057/biodiv/d211110
DO - 10.13057/biodiv/d211110
M3 - Article
AN - SCOPUS:85092252258
SN - 1412-033X
VL - 21
SP - 5065
EP - 5071
JO - Biodiversitas
JF - Biodiversitas
IS - 11
ER -