Effects of oxygen insufflation during pilocarpine-induced status epilepticus on mortality, tissue damage and seizures

Lorenz Müller, Steffen Müller, Tina Sellmann, Linda Groeneweg, Tursonjan Tokay, Rüdiger Köhling, Timo Kirschstein

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Purpose: This prospective, randomized study was performed to investigate the effects of oxygen (O2) treatment during sustained epileptic activity on mortality, subsequent seizure frequency, and neuronal damage. Methods: Status epilepticus (SE) was induced by intraperitoneal injection of 340mg/kg pilocarpine, and terminated by diazepam after 40min. During SE, rats were randomized to O2 treatment (insufflation rate of 1.5l/min O2) during SE or normal air conditions. Outcome measures were SE-related mortality, seizure occurrence, mossy fiber sprouting, neuronal cell loss and expression of 27-kDa heat-shock protein (Hsp27). Results: O2-treated and O2-untreated animals did not differ with respect to SE latency, diazepam dose required to stop SE. While 7/38 rats died during SE in the O2-untreated group, very little mortality (1/38) occurred in the O2-treated group (P<0.05). However, within 1h after SE termination, seven O2-treated rats died which was not observed in the O2-untreated group indicating no significant difference in overall mortality. There was a tendency towards lower seizure rate in the O2-treated group at one month after pilocarpine-induced SE. Three months after SE, however, seizure rates were no longer different between both groups. Moreover, mossy fiber sprouting, neuronal cell loss and Hsp27 expression did not differ between O2-treated and O2-untreated groups. Conclusion: Our findings indicate that O2 treatment might delay the relative risk of epileptic seizures following an initial brain injury, but it may also lead to a rather unfavorably increased heterogeneity of epileptogenesis in experimental studies.

Original languageEnglish
Pages (from-to)90-97
Number of pages8
JournalEpilepsy Research
Issue number1
Publication statusPublished - Jan 2014


  • Heat-shock protein
  • Oxygen
  • Pilocarpine
  • Status epilepticus
  • Timm stain

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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