Electrochemical impedance spectroscopy for black lipid membranes fused with channel protein supported on solid-state nanopore

Muhammad S. Khan, Noura S. Dosoky, Bakhrom K. Berdiev, John D. Williams

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Black lipid membranes (BLMs) have been used for detecting single-channel activities of pore-forming peptides and ion channels. However, the short lifetimes and poor mechanical stability of suspended bilayers limit their applications in high throughput electrophysiological experiments. In this work, we present a synthetic solid-state nanopore functionalized with BLM fused with channel protein. A nanopore with diameter of ~180 nm was electrochemically fabricated in a thin silicon membrane. Folding and painting techniques were demonstrated for production of stable suspended BLMs followed by incorporation of transmembrane protein, ENaC. Membrane formation was confirmed by employing electrochemical impedance spectroscopy (EIS) in the frequency regime of 10−2–105 Hz. Results show that electrochemically fabricated solid state nanopore support resulted in excellent membrane stability, with >1 GΩ of up to 72 and 41 h for painting and folding techniques, respectively. After fusion of ENaC channel protein, the BLM exhibits the stability of ~5 h. We anticipate that such a solid-state nanopore with diameter in the range of 150–200 nm and thickness <1 µm could be a potential platform to enhance the throughput of ion-channel characterization using BLMs.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalEuropean Biophysics Journal
DOIs
Publication statusAccepted/In press - Aug 1 2016

Keywords

  • Black lipid membranes
  • Electrochemical impedance spectroscopy
  • ENaC
  • Nanobiosensor
  • Silicon
  • Solid-state nanopore
  • Transmembrane protein

ASJC Scopus subject areas

  • Biophysics

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