Issledovanie élektronnogo transporta v fotosinteticheskikh sistemakh metodom élektronnogo paramagnitnogo rezonansa. IX. Temperaturnaia zavisimost' kinetiki fotoindutsirovannykh okislitel'novosstanovitel'nykh prevrashcheniǐ P700 pri osveshchenii khloroplastov bobov vspyshkami sveta razlichnoǐ dliltel'nosti.

Translated title of the contribution: Electron spin resonance of electron transport in photosynthetic systems. IX. Temperature dependence of the kinetics of P700 redox transients in bean chloroplasts induced by flashes of different duration

A. N. Tikhonov, G. B. Khomutov, E. K. Ruuge

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The temperature dependence of the kinetics of P700 redox transients in bean chloroplasts was studied. The flashes of white light with different duration (7 microseconds, 0.5 and 0.75 ms) were fired simultaneously with the background continuous far red light (lambda max = 707 nm). It was shown that the rate of P700+ reduction was temperature dependent and increased with the rise of the concentration of the reductants in the electron transport chain between photosystems. Photosystem 2 donates electrons to P700+ at temperatures from -5 to 45 degrees C under various modes of flash illumination. Experiments with spin labels showed that there were correlation between the physical state of lipids in the chloroplasts membrane and the rates of different steps of electron transport from photosystem 2 to photosystem 1--plastoquinone reduction by photosystem 2 and plastoquinol oxidation by photosystem 1. We assume that the rates of electron transport reaction of the plastoquinone shuttle are controlled by diffusion of plastoquinone and plastoquinole in the hydrophobic part of the thylakoid membrane. Additional evidence in support of that proposal was obtained from the temperature dependence of light induced spin label reduction which occurred due to its interaction with the plastoquinol of plastosemiquinone.

Original languageRussian
Pages (from-to)157-172
Number of pages16
JournalMolekulyarnaya Biologiya
Volume14
Issue number1
Publication statusPublished - 1980
Externally publishedYes

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Plastoquinone
Electron Spin Resonance Spectroscopy
Chloroplasts
Electron Transport
Oxidation-Reduction
Spin Labels
Temperature
Light
Thylakoids
Reducing Agents
Lighting
Electrons
Lipids
Membranes
plastoquinol

ASJC Scopus subject areas

  • Molecular Biology

Cite this

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title = "Issledovanie {\'e}lektronnogo transporta v fotosinteticheskikh sistemakh metodom {\'e}lektronnogo paramagnitnogo rezonansa. IX. Temperaturnaia zavisimost' kinetiki fotoindutsirovannykh okislitel'novosstanovitel'nykh prevrashcheniǐ P700 pri osveshchenii khloroplastov bobov vspyshkami sveta razlichnoǐ dliltel'nosti.",
abstract = "The temperature dependence of the kinetics of P700 redox transients in bean chloroplasts was studied. The flashes of white light with different duration (7 microseconds, 0.5 and 0.75 ms) were fired simultaneously with the background continuous far red light (lambda max = 707 nm). It was shown that the rate of P700+ reduction was temperature dependent and increased with the rise of the concentration of the reductants in the electron transport chain between photosystems. Photosystem 2 donates electrons to P700+ at temperatures from -5 to 45 degrees C under various modes of flash illumination. Experiments with spin labels showed that there were correlation between the physical state of lipids in the chloroplasts membrane and the rates of different steps of electron transport from photosystem 2 to photosystem 1--plastoquinone reduction by photosystem 2 and plastoquinol oxidation by photosystem 1. We assume that the rates of electron transport reaction of the plastoquinone shuttle are controlled by diffusion of plastoquinone and plastoquinole in the hydrophobic part of the thylakoid membrane. Additional evidence in support of that proposal was obtained from the temperature dependence of light induced spin label reduction which occurred due to its interaction with the plastoquinol of plastosemiquinone.",
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AU - Ruuge, E. K.

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