Electrostatic interactions in catalytic centers of F1-ATPase

Alexandra F. Pogrebnaya, Yury M. Romanovsky, Alexander N. Tikhonov

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

F1-ATPase is one of the most important enzymes of membrane bioenergetics. F1-ATPase is the constituent complex that provides the ATP formation from ADP and inorganic phosphate (Pi) at the expense of energy of electrochemical gradient of hydrogen ions generated across the energy transducing mitochondrial, chloroplast or bacterial membrane. F1-ATPase is a reversible molecular machine that can work as a proton pump due to energy released in the course of ATP hydrolysis (ATPase reaction). The unusual feature of this enzyme is that is operates as a rotary molecular motor. Recently, using the fluorescence microscopy method for the real time visualization of molecular mobility of individual molecules, it was demonstrated directly that the ATP hydrolysis by F1-ATPase is accompanied by unidirectional rotations of mobile subunits (rotor) of F1F0-ATP synthase. In this work, we calculated the contribution of electrostatic interactions between charged groups of a substrate (MgATP), products molecules (MgADP and Pi), and charged amino acid residuals of ATPase molecule to the energy changes associated with the substrate binding and their chemical transformations in the catalytic centers located at the interface of α and β subunits of the enzyme (oligomer complex α3β3γ of bovine mitochondria ATPase). A catalytic cycle of ATP hydrolysis considered in our work includes conformational changes of α and β subunits caused by unidirectional rotations of an eccentric γ subunit. The knowledge of energy characteristics and force field in catalytic center of an enzyme in different conformational states may be important for further simulation dynamic properties of ATP synthase complex.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsV.V. Tuchin
Pages27-35
Number of pages9
Volume5068
Publication statusPublished - 2002
Externally publishedYes
EventSaratov Fall Meeting 2002 Optical Technologies in Biophysics and Medicine IV - Saratov, Russian Federation
Duration: Oct 1 2002Oct 4 2002

Other

OtherSaratov Fall Meeting 2002 Optical Technologies in Biophysics and Medicine IV
CountryRussian Federation
CitySaratov
Period10/1/0210/4/02

Keywords

  • ATP hydrolysis
  • Electrostatic energy
  • F-ATPase
  • Molecular motor

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

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