ENaC subunit-subunit interactions and inhibition by syntaxin 1A

Bakhrom K. Berdiev, Biljana Jovov, Ward C. Tucker, Anjaparavanda P. Naren, Catherine M. Fuller, Edwin R. Chapman, Dale J. Benos

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Amiloride-sensitive epithelial Na+ channels (ENaCs) are subject to modulation by many factors. Recent data have also linked the N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) machinery to this regulation of ENaC, but the molecular mechanisms that underlie this modulation are poorly understood. In this study, we demonstrate that syntaxin 1A physically interacts with ENaC and functionally regulates ENaC activity. Syntaxin 1A was able to coimmunoprecipitate in vitro-translated γ-ENaC, but not α- or β-ENaC. Also, using antibodies raised against α-, β-, or γ-ENaC, we detected syntaxin 1A in immunoprecipitates from Madin-Darby canine kidney cells stably transfected with αβγ -ENaC. In bilayers, syntaxin 1A inhibited ENaC, and this syntaxin 1A modulation of ENaC activity was eliminated by truncations of cytoplasmic domains of the ENaC subunits. Our findings provide evidence for a direct physical interaction between ENaC and syntaxin 1A and suggest involvement of ENaC's cytoplasmic domains in functional modulation of ENaC activity by syntaxin 1A.

Original languageEnglish
JournalAmerican Journal of Physiology - Renal Physiology
Volume286
Issue number6 55-6
DOIs
Publication statusPublished - Jun 2004
Externally publishedYes

Fingerprint

Syntaxin 1
Epithelial Sodium Channels
N-Ethylmaleimide-Sensitive Proteins
SNARE Proteins
Madin Darby Canine Kidney Cells
Amiloride

Keywords

  • Bilayers
  • Channels
  • Interaction
  • Membrane

ASJC Scopus subject areas

  • Physiology

Cite this

Berdiev, B. K., Jovov, B., Tucker, W. C., Naren, A. P., Fuller, C. M., Chapman, E. R., & Benos, D. J. (2004). ENaC subunit-subunit interactions and inhibition by syntaxin 1A. American Journal of Physiology - Renal Physiology, 286(6 55-6). https://doi.org/10.1152/ajprenal.00344.2003

ENaC subunit-subunit interactions and inhibition by syntaxin 1A. / Berdiev, Bakhrom K.; Jovov, Biljana; Tucker, Ward C.; Naren, Anjaparavanda P.; Fuller, Catherine M.; Chapman, Edwin R.; Benos, Dale J.

In: American Journal of Physiology - Renal Physiology, Vol. 286, No. 6 55-6, 06.2004.

Research output: Contribution to journalArticle

Berdiev, BK, Jovov, B, Tucker, WC, Naren, AP, Fuller, CM, Chapman, ER & Benos, DJ 2004, 'ENaC subunit-subunit interactions and inhibition by syntaxin 1A', American Journal of Physiology - Renal Physiology, vol. 286, no. 6 55-6. https://doi.org/10.1152/ajprenal.00344.2003
Berdiev, Bakhrom K. ; Jovov, Biljana ; Tucker, Ward C. ; Naren, Anjaparavanda P. ; Fuller, Catherine M. ; Chapman, Edwin R. ; Benos, Dale J. / ENaC subunit-subunit interactions and inhibition by syntaxin 1A. In: American Journal of Physiology - Renal Physiology. 2004 ; Vol. 286, No. 6 55-6.
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