Encapsulation of various recombinant mammalian cell types in different alginate microcapsules

Michael Peirone, Colin J D Ross, Gonzalo Hortelano, John L. Brash, Patricia L. Chang

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Microencapsulation of recombinant 'universal' cells with immunoprotective membranes is an alternate approach to somatic gene therapy. Therapeutic gene products secreted by these cells can be delivered to different patients without immunosuppression or genetic modification of the host's cells. The encapsulation of different mammalian cell types (epithelial cells, fibroblasts, and myoblasts) is compared among three alginate-based microcapsules: (1) calcium-linked alginate microcapsules with a solubilized core and a poly-L-lysine - alginate-taminated surface; (2) barium-linked alginate beads with a gelled core; and (3) a hybrid formulation of barium- linked alginate beads with a poly-L-lysine-alginate-laminated surface. The mechanical stability of the different microcapsule types, as measured with a cone-and-plate shearing apparatus, was superior in the two barium-linked alginate beads. All cell types maintained high viability (65-90%) in culture after encapsulation. The recombinant gene products secreted by these cells (human growth hormone MW = 22,000, human factor IX MW = 57,000, and murine β-glucuronidase MW = 300,000) were able to traverse the three microcapsule types at similar rates. Cell numbers within the microcapsules increased twofold to > 20-fold over 4 weeks, depending on the cell type. Epithelial and myoblast cell numbers were not affected by microcapsule formulation; however, fibroblasts proliferated the most in the calcium-linked alginate spheres. These results show that for culturing fibroblasts in a mechanically stable environment the classical calcium-linked microcapsules are adequate. However, where mechanical stability is a more critical requirement, the solid barium- linked gelled beads are more appropriate choices.

Original languageEnglish
Pages (from-to)587-596
Number of pages10
JournalJournal of Biomedical Materials Research
Volume42
Issue number4
DOIs
Publication statusPublished - Dec 15 1998
Externally publishedYes

Fingerprint

Alginate
Encapsulation
Capsules
Cells
Barium
Fibroblasts
Mechanical stability
Calcium
Genes
Microencapsulation
Gene therapy
Factor IX
Human Growth Hormone
Glucuronidase
Human engineering
alginic acid
Shearing
Cell culture
Hormones
Cones

Keywords

  • β- glucuronidase
  • Factor IX
  • Gene therapy
  • Human growth hormone
  • Immunoisolation

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Encapsulation of various recombinant mammalian cell types in different alginate microcapsules. / Peirone, Michael; Ross, Colin J D; Hortelano, Gonzalo; Brash, John L.; Chang, Patricia L.

In: Journal of Biomedical Materials Research, Vol. 42, No. 4, 15.12.1998, p. 587-596.

Research output: Contribution to journalArticle

Peirone, Michael ; Ross, Colin J D ; Hortelano, Gonzalo ; Brash, John L. ; Chang, Patricia L. / Encapsulation of various recombinant mammalian cell types in different alginate microcapsules. In: Journal of Biomedical Materials Research. 1998 ; Vol. 42, No. 4. pp. 587-596.
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