TY - JOUR
T1 - Engineering of cell membranes with a bisphosphonate-containing polymer using ATRP synthesis for bone targeting
AU - D'Souza, Sonia
AU - Murata, Hironobu
AU - Jose, Moncy V.
AU - Askarova, Sholpan
AU - Yantsen, Yuliya
AU - Andersen, Jill D.
AU - Edington, Collin D.J.
AU - Clafshenkel, William P.
AU - Koepsel, Richard R.
AU - Russell, Alan J.
N1 - Funding Information:
Funding Sources: This work was supported by the Advanced Regenerative Medicine grant from Pittsburgh Tissue Engineering Initiative (Grant no. W81XWH-10-1-0618 ), The Institute for Transfusion Medicine , and by an award from the Government of the Republic of Kazakhstan via Nazarbayev University. NMR instrumentation at CMU was partially supported by NSF ( CHE-0130903 and CHE-1039870 ).
Publisher Copyright:
© 2014 Elsevier Ltd.
PY - 2014
Y1 - 2014
N2 - The field of polymer-based membrane engineering has expanded since we first demonstrated the reaction of N-hydroxysuccinimide ester-terminated polymers with cells and tissues almost two decades ago. One remaining obstacle, especially for conjugation of polymers to cells, has been that exquisite control over polymer structure and functionality has not been used to influence the behavior of cells. Herein, we describe a multifunctional atom transfer radical polymerization initiator and its use to synthesize water-soluble polymers that are modified with bisphosphonate side chains and then covalently bound to the surface of live cells. The polymers contained between 1.7 and 3.1 bisphosphonates per chain and were shown to bind to hydroxyapatite crystals with kinetics similar to free bisphosphonate binding. We engineered the membranes of both HL-60 cells and mesenchymal stem cells in order to impart polymer-guided bone adhesion properties on the cells. Covalent coupling of the polymer to the non-adherent HL-60 cell line or mesenchymal stem cells was non-toxic by proliferation assays and enhanced the binding of these cells to bone.
AB - The field of polymer-based membrane engineering has expanded since we first demonstrated the reaction of N-hydroxysuccinimide ester-terminated polymers with cells and tissues almost two decades ago. One remaining obstacle, especially for conjugation of polymers to cells, has been that exquisite control over polymer structure and functionality has not been used to influence the behavior of cells. Herein, we describe a multifunctional atom transfer radical polymerization initiator and its use to synthesize water-soluble polymers that are modified with bisphosphonate side chains and then covalently bound to the surface of live cells. The polymers contained between 1.7 and 3.1 bisphosphonates per chain and were shown to bind to hydroxyapatite crystals with kinetics similar to free bisphosphonate binding. We engineered the membranes of both HL-60 cells and mesenchymal stem cells in order to impart polymer-guided bone adhesion properties on the cells. Covalent coupling of the polymer to the non-adherent HL-60 cell line or mesenchymal stem cells was non-toxic by proliferation assays and enhanced the binding of these cells to bone.
KW - ATRP
KW - Bone targeting polymer
KW - Cell reactive polymers
KW - Membrane engineering
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U2 - 10.1016/j.biomaterials.2014.07.041
DO - 10.1016/j.biomaterials.2014.07.041
M3 - Article
C2 - 25150889
AN - SCOPUS:84961288380
SN - 0142-9612
VL - 35
SP - 9447
EP - 9458
JO - Biomaterials
JF - Biomaterials
IS - 35
ER -