Enzyme-Catalyzed Crosslinking in a Partly Frozen State

A New Way to Produce Supermacroporous Protein Structures

Harald Kirsebom, Linda Elowsson, Dmitriy Berillo, Séverine Cozzi, Ilyas Inci, Erhan Piskin, Igor Yu Galaev, Bo Mattiasson

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In this study a new way to produce supermacroporous protein structures was investigated. Enzyme-mediated crosslinking of gelatin or casein was performed in a partly frozen state, which yielded stable, protein-based cryogels. The reaction kinetics for the formation of cryogels were found to be fairly slow, most likely due to the low temperature (-12°C) used or due to an increased viscosity owing to the cryo-concentration taking place. The produced cryogels were characterized with regards to their physical properties and in vitro degradation. Furthermore, cryogels produced from gelatin and casein were evaluated as potential scaffolds by fibroblast cultivation to confirm their in vitro biocompatibility. Gelatin- and casein-based scaffolds both supported cell proliferation and migration through the scaffold.

Original languageEnglish
Pages (from-to)67-76
Number of pages10
JournalMacromolecular Bioscience
Volume13
Issue number1
DOIs
Publication statusPublished - Jan 2013
Externally publishedYes

Fingerprint

Cryogels
Casein
Scaffolds (biology)
Crosslinking
Gelatin
Enzymes
Caseins
Proteins
Cell proliferation
Fibroblasts
Biocompatibility
Reaction kinetics
Physical properties
Viscosity
Scaffolds
Cell Movement
Degradation
Cell Proliferation
Temperature

Keywords

  • Biocompatibility
  • Enzymatic crosslinking
  • Hydrogels
  • Protein-based scaffolds
  • Supermacroporous hydrogels

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Kirsebom, H., Elowsson, L., Berillo, D., Cozzi, S., Inci, I., Piskin, E., ... Mattiasson, B. (2013). Enzyme-Catalyzed Crosslinking in a Partly Frozen State: A New Way to Produce Supermacroporous Protein Structures. Macromolecular Bioscience, 13(1), 67-76. https://doi.org/10.1002/mabi.201200343

Enzyme-Catalyzed Crosslinking in a Partly Frozen State : A New Way to Produce Supermacroporous Protein Structures. / Kirsebom, Harald; Elowsson, Linda; Berillo, Dmitriy; Cozzi, Séverine; Inci, Ilyas; Piskin, Erhan; Galaev, Igor Yu; Mattiasson, Bo.

In: Macromolecular Bioscience, Vol. 13, No. 1, 01.2013, p. 67-76.

Research output: Contribution to journalArticle

Kirsebom, H, Elowsson, L, Berillo, D, Cozzi, S, Inci, I, Piskin, E, Galaev, IY & Mattiasson, B 2013, 'Enzyme-Catalyzed Crosslinking in a Partly Frozen State: A New Way to Produce Supermacroporous Protein Structures', Macromolecular Bioscience, vol. 13, no. 1, pp. 67-76. https://doi.org/10.1002/mabi.201200343
Kirsebom, Harald ; Elowsson, Linda ; Berillo, Dmitriy ; Cozzi, Séverine ; Inci, Ilyas ; Piskin, Erhan ; Galaev, Igor Yu ; Mattiasson, Bo. / Enzyme-Catalyzed Crosslinking in a Partly Frozen State : A New Way to Produce Supermacroporous Protein Structures. In: Macromolecular Bioscience. 2013 ; Vol. 13, No. 1. pp. 67-76.
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