Enzyme-Catalyzed Crosslinking in a Partly Frozen State: A New Way to Produce Supermacroporous Protein Structures

Harald Kirsebom, Linda Elowsson, Dmitriy Berillo, Séverine Cozzi, Ilyas Inci, Erhan Piskin, Igor Yu Galaev, Bo Mattiasson

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


In this study a new way to produce supermacroporous protein structures was investigated. Enzyme-mediated crosslinking of gelatin or casein was performed in a partly frozen state, which yielded stable, protein-based cryogels. The reaction kinetics for the formation of cryogels were found to be fairly slow, most likely due to the low temperature (-12°C) used or due to an increased viscosity owing to the cryo-concentration taking place. The produced cryogels were characterized with regards to their physical properties and in vitro degradation. Furthermore, cryogels produced from gelatin and casein were evaluated as potential scaffolds by fibroblast cultivation to confirm their in vitro biocompatibility. Gelatin- and casein-based scaffolds both supported cell proliferation and migration through the scaffold.

Original languageEnglish
Pages (from-to)67-76
Number of pages10
JournalMacromolecular Bioscience
Issue number1
Publication statusPublished - Jan 1 2013
Externally publishedYes


  • Biocompatibility
  • Enzymatic crosslinking
  • Hydrogels
  • Protein-based scaffolds
  • Supermacroporous hydrogels

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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