Epigenetic regulation of mammalian pericentric heterochromatin in vivo by HP1

Niki Kourmouli, Yuh-man Sun, Sjaak van der Sar, Prim B Singh, Jeremy P Brown

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

We developed a model system whereby HP1 can be targeted to pericentric heterochromatin in ES cells lacking Suv(3)9h1/2 histone methyltransferase (HMTase) activities. HP1 so targeted can reconstitute tri-methylated lysine 9 of histone H3 (Me(3)K9H3) and tri-methylated lysine 20 of histone H4 (Me(3)K20H4) at pericentric heterochromatin, indicating that HP1 can regulate the distribution of these histone modifications in vivo. Both homo- and hetero-typic interactions between the HP1 isotypes were demonstrated in vivo as were HP1 interactions with the ESET/SETDB1 HMTase and the ATRX chromatin remodelling enzyme. We conclude that HP1 not only "deciphers" the histone code but can also "encode it".

Original languageEnglish
Pages (from-to)901-7
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume337
Issue number3
DOIs
Publication statusPublished - Nov 25 2005

Keywords

  • Animals
  • Cell Line
  • Centromere
  • Chromosomal Proteins, Non-Histone
  • Epigenesis, Genetic
  • Gene Expression Regulation, Developmental
  • Heterochromatin
  • Histone Code
  • Histones
  • Mice
  • Stem Cells
  • Journal Article

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