EPR study of electron transport in the cyanobacterium Synechocystis sp. PCC 6803

Oxygen-dependent interrelations between photosynthetic and respiratory electron transport chains

Boris V. Trubitsin, Vasilii V. Ptushenko, Olga A. Koksharova, Mahir D. Mamedov, Liya A. Vitukhnovskaya, Igor A. Grigor'ev, Alexey Yu Semenov, Alexander N. Tikhonov

Research output: Contribution to journalArticle

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Abstract

In this work, we investigated electron transport processes in the cyanobacterium Synechocystis sp. PCC 6803, with a special emphasis focused on oxygen-dependent interrelations between photosynthetic and respiratory electron transport chains. Redox transients of the photosystem I primary donor P700 and oxygen exchange processes were measured by the EPR method under the same experimental conditions. To discriminate between the factors controlling electron flow through photosynthetic and respiratory electron transport chains, we compared the P700 redox transients and oxygen exchange processes in wild type cells and mutants with impaired photosystem II and terminal oxidases (CtaI, CydAB, CtaDEII). It was shown that the rates of electron flow through both photosynthetic and respiratory electron transport chains strongly depended on the transmembrane proton gradient and oxygen concentration in cell suspension. Electron transport through photosystem I was controlled by two main mechanisms: (i) oxygen-dependent acceleration of electron transfer from photosystem I to NADP+, and (ii) slowing down of electron flow between photosystem II and photosystem I governed by the intrathylakoid pH. Inhibitor analysis of P700 redox transients led us to the conclusion that electron fluxes from dehydrogenases and from cyclic electron transport pathway comprise 20-30% of the total electron flux from the intersystem electron transport chain to P700 +.

Original languageEnglish
Pages (from-to)238-249
Number of pages12
JournalBBA - Bioenergetics
Volume1708
Issue number2
DOIs
Publication statusPublished - Jun 30 2005
Externally publishedYes

Fingerprint

Respiratory Transport
Synechocystis
Cyanobacteria
Electron Transport
Paramagnetic resonance
Photosystem I Protein Complex
Oxygen
Electrons
Oxidation-Reduction
Photosystem II Protein Complex
Oxidoreductases
Fluxes
NADP
Protons
Suspensions

Keywords

  • Cyanobacteria
  • Electron paramagnetic resonance
  • Electron transport control

ASJC Scopus subject areas

  • Biophysics

Cite this

EPR study of electron transport in the cyanobacterium Synechocystis sp. PCC 6803 : Oxygen-dependent interrelations between photosynthetic and respiratory electron transport chains. / Trubitsin, Boris V.; Ptushenko, Vasilii V.; Koksharova, Olga A.; Mamedov, Mahir D.; Vitukhnovskaya, Liya A.; Grigor'ev, Igor A.; Semenov, Alexey Yu; Tikhonov, Alexander N.

In: BBA - Bioenergetics, Vol. 1708, No. 2, 30.06.2005, p. 238-249.

Research output: Contribution to journalArticle

Trubitsin, Boris V. ; Ptushenko, Vasilii V. ; Koksharova, Olga A. ; Mamedov, Mahir D. ; Vitukhnovskaya, Liya A. ; Grigor'ev, Igor A. ; Semenov, Alexey Yu ; Tikhonov, Alexander N. / EPR study of electron transport in the cyanobacterium Synechocystis sp. PCC 6803 : Oxygen-dependent interrelations between photosynthetic and respiratory electron transport chains. In: BBA - Bioenergetics. 2005 ; Vol. 1708, No. 2. pp. 238-249.
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abstract = "In this work, we investigated electron transport processes in the cyanobacterium Synechocystis sp. PCC 6803, with a special emphasis focused on oxygen-dependent interrelations between photosynthetic and respiratory electron transport chains. Redox transients of the photosystem I primary donor P700 and oxygen exchange processes were measured by the EPR method under the same experimental conditions. To discriminate between the factors controlling electron flow through photosynthetic and respiratory electron transport chains, we compared the P700 redox transients and oxygen exchange processes in wild type cells and mutants with impaired photosystem II and terminal oxidases (CtaI, CydAB, CtaDEII). It was shown that the rates of electron flow through both photosynthetic and respiratory electron transport chains strongly depended on the transmembrane proton gradient and oxygen concentration in cell suspension. Electron transport through photosystem I was controlled by two main mechanisms: (i) oxygen-dependent acceleration of electron transfer from photosystem I to NADP+, and (ii) slowing down of electron flow between photosystem II and photosystem I governed by the intrathylakoid pH. Inhibitor analysis of P700 redox transients led us to the conclusion that electron fluxes from dehydrogenases and from cyclic electron transport pathway comprise 20-30{\%} of the total electron flux from the intersystem electron transport chain to P700 +.",
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