Etched fiber bragg grating biosensor functionalized with aptamers for detection of thrombin

Aliya Bekmurzayeva, Kanat Dukenbayev, Madina Shaimerdenova, Ildar Bekniyazov, Takhmina Ayupova, Marzhan Sypabekova, Carlo Molardi, Daniele Tosi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A biosensor based on an etched Fiber Bragg Grating (EFBG) for thrombin detection is reported. The sensing system is based on a Fiber Bragg Grating (FBG) with a Bragg wavelength of 1550 nm, wet-etched in hydrofluoric acid (HF) for ~27 min, to achieve sensitivity to a refractive index (RI) of 17.4 nm/RIU (refractive index unit). Subsequently, in order to perform a selective detection of thrombin, the EFBG has been functionalized with silane-coupling agent 3-(aminopropyl)triethoxysilane (APTES) and a cross-linker, glutaraldehyde, for the immobilization of thrombin-binding aptamer. The biosensor has been validated for thrombin detection in concentrations ranging from 10 nM to 80 nM. The proposed sensor presents advantages with respect to other sensor configurations, based on plasmonic resonant tilted FBG or Long Period Grating (LPG), for thrombin detection. Firstly, fabricating an EFBG only requires chemical etching. Moreover, the functionalization method used in this study (silanization) allows the avoidance of complicated and expensive fabrications, such as thin film sputtering or chemical vapor deposition. Due to their characteristics, EFBG sensors are easier to multiplex and can be used in vivo. This opens new possibilities for the detection of thrombin in clinical settings.

Original languageEnglish
Article number4298
JournalSensors (Switzerland)
Volume18
Issue number12
DOIs
Publication statusPublished - Dec 1 2018

Fingerprint

thrombin
Biosensing Techniques
Fiber Bragg gratings
bioinstrumentation
Biosensors
Thrombin
Bragg gratings
fibers
Refractometry
sensors
Refractive index
Sensors
Hydrofluoric Acid
Silanes
refractivity
Glutaral
Hydrofluoric acid
Immobilization
avoidance
Coupling agents

Keywords

  • Aptamer
  • Biosensor
  • Fiber bragg gratings (FBG)
  • Optical fiber biosensors
  • Refractive index sensor
  • Thrombin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Atomic and Molecular Physics, and Optics
  • Biochemistry
  • Instrumentation
  • Electrical and Electronic Engineering

Cite this

Etched fiber bragg grating biosensor functionalized with aptamers for detection of thrombin. / Bekmurzayeva, Aliya; Dukenbayev, Kanat; Shaimerdenova, Madina; Bekniyazov, Ildar; Ayupova, Takhmina; Sypabekova, Marzhan; Molardi, Carlo; Tosi, Daniele.

In: Sensors (Switzerland), Vol. 18, No. 12, 4298, 01.12.2018.

Research output: Contribution to journalArticle

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