Evaluation of reliability of FISH versus brightfield dual-probe in situ hybridization (BDISH) for frontline assessment of HER2 status in breast cancer samples in a community setting

Influence of poor tissue preservation

Beatriz N. Schiavon, Bharat Jasani, Louise De Brot, José Vassallo, Aline Damascena, Julio Cirullo-Neto, José Ivanildo Neves, Fernando Augusto Soares, Helenice Gobbi, Rafael Malagoli Rocha

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

AIMS: To evaluate the reliability of novel brightfield microscopy-based dual in situ hybridization (BDISH) methods for frontline HER2 status analysis in selected suboptimally preserved breast cancer tissue samples reflecting of the worst scenario in a community. METHODS AND RESULTS: A total of 320 morphologically poorly preserved breast invasive ductal carcinomas from the archives of 2 tertiary institutions in Brazil were selected for a tissue microarray-based analysis. 4B5 antibody was used for immunohistochemistry. Fluorescence in situ hybridization (FISH), DuoCISH, ZytoDot CISH, and silver in situ hybridization (SISH) were performed and compared. The highest agreement was observed between SISH and FISH. In addition, SISH was easier to assess in both amplified and nonamplified cases when compared with the other chromogenic methods, due to the sharpness of its dots. DuoCISH produced false-positive results, associated with thicker ill-defined dots, causing poor distinction between nonamplification and low amplification. ZytoDot CISH showed lower sensitivity, with increased frequency of false-positive results. CONCLUSIONS: SISH is the most reliable of the BDISH methods, with sensitivity and specificity highly comparable with FISH. It is also less deleterious than other BDISH methods, producing signals that were more distinct and therefore more readily analyzable even in poorly preserved tissue.

Original languageEnglish
Pages (from-to)1489-1496
Number of pages8
JournalAmerican Journal of Surgical Pathology
Volume36
Issue number10
DOIs
Publication statusPublished - Oct 2012
Externally publishedYes

Fingerprint

Tissue Preservation
Fluorescence In Situ Hybridization
In Situ Hybridization
Breast Neoplasms
Silver
Tissue Array Analysis
Carcinoma, Ductal, Breast
Brazil
Microscopy
Immunohistochemistry
Sensitivity and Specificity
Antibodies

Keywords

  • breast cancer
  • HER2
  • in situ hybridization
  • SISH

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine
  • Surgery

Cite this

Evaluation of reliability of FISH versus brightfield dual-probe in situ hybridization (BDISH) for frontline assessment of HER2 status in breast cancer samples in a community setting : Influence of poor tissue preservation. / Schiavon, Beatriz N.; Jasani, Bharat; De Brot, Louise; Vassallo, José; Damascena, Aline; Cirullo-Neto, Julio; Ivanildo Neves, José; Augusto Soares, Fernando; Gobbi, Helenice; Malagoli Rocha, Rafael.

In: American Journal of Surgical Pathology, Vol. 36, No. 10, 10.2012, p. 1489-1496.

Research output: Contribution to journalArticle

Schiavon, Beatriz N. ; Jasani, Bharat ; De Brot, Louise ; Vassallo, José ; Damascena, Aline ; Cirullo-Neto, Julio ; Ivanildo Neves, José ; Augusto Soares, Fernando ; Gobbi, Helenice ; Malagoli Rocha, Rafael. / Evaluation of reliability of FISH versus brightfield dual-probe in situ hybridization (BDISH) for frontline assessment of HER2 status in breast cancer samples in a community setting : Influence of poor tissue preservation. In: American Journal of Surgical Pathology. 2012 ; Vol. 36, No. 10. pp. 1489-1496.
@article{f05c55eec29a4bd489988acb19f2e451,
title = "Evaluation of reliability of FISH versus brightfield dual-probe in situ hybridization (BDISH) for frontline assessment of HER2 status in breast cancer samples in a community setting: Influence of poor tissue preservation",
abstract = "AIMS: To evaluate the reliability of novel brightfield microscopy-based dual in situ hybridization (BDISH) methods for frontline HER2 status analysis in selected suboptimally preserved breast cancer tissue samples reflecting of the worst scenario in a community. METHODS AND RESULTS: A total of 320 morphologically poorly preserved breast invasive ductal carcinomas from the archives of 2 tertiary institutions in Brazil were selected for a tissue microarray-based analysis. 4B5 antibody was used for immunohistochemistry. Fluorescence in situ hybridization (FISH), DuoCISH, ZytoDot CISH, and silver in situ hybridization (SISH) were performed and compared. The highest agreement was observed between SISH and FISH. In addition, SISH was easier to assess in both amplified and nonamplified cases when compared with the other chromogenic methods, due to the sharpness of its dots. DuoCISH produced false-positive results, associated with thicker ill-defined dots, causing poor distinction between nonamplification and low amplification. ZytoDot CISH showed lower sensitivity, with increased frequency of false-positive results. CONCLUSIONS: SISH is the most reliable of the BDISH methods, with sensitivity and specificity highly comparable with FISH. It is also less deleterious than other BDISH methods, producing signals that were more distinct and therefore more readily analyzable even in poorly preserved tissue.",
keywords = "breast cancer, HER2, in situ hybridization, SISH",
author = "Schiavon, {Beatriz N.} and Bharat Jasani and {De Brot}, Louise and Jos{\'e} Vassallo and Aline Damascena and Julio Cirullo-Neto and {Ivanildo Neves}, Jos{\'e} and {Augusto Soares}, Fernando and Helenice Gobbi and {Malagoli Rocha}, Rafael",
year = "2012",
month = "10",
doi = "10.1097/PAS.0b013e3182635987",
language = "English",
volume = "36",
pages = "1489--1496",
journal = "American Journal of Surgical Pathology",
issn = "0147-5185",
publisher = "Lippincott Williams and Wilkins",
number = "10",

}

TY - JOUR

T1 - Evaluation of reliability of FISH versus brightfield dual-probe in situ hybridization (BDISH) for frontline assessment of HER2 status in breast cancer samples in a community setting

T2 - Influence of poor tissue preservation

AU - Schiavon, Beatriz N.

AU - Jasani, Bharat

AU - De Brot, Louise

AU - Vassallo, José

AU - Damascena, Aline

AU - Cirullo-Neto, Julio

AU - Ivanildo Neves, José

AU - Augusto Soares, Fernando

AU - Gobbi, Helenice

AU - Malagoli Rocha, Rafael

PY - 2012/10

Y1 - 2012/10

N2 - AIMS: To evaluate the reliability of novel brightfield microscopy-based dual in situ hybridization (BDISH) methods for frontline HER2 status analysis in selected suboptimally preserved breast cancer tissue samples reflecting of the worst scenario in a community. METHODS AND RESULTS: A total of 320 morphologically poorly preserved breast invasive ductal carcinomas from the archives of 2 tertiary institutions in Brazil were selected for a tissue microarray-based analysis. 4B5 antibody was used for immunohistochemistry. Fluorescence in situ hybridization (FISH), DuoCISH, ZytoDot CISH, and silver in situ hybridization (SISH) were performed and compared. The highest agreement was observed between SISH and FISH. In addition, SISH was easier to assess in both amplified and nonamplified cases when compared with the other chromogenic methods, due to the sharpness of its dots. DuoCISH produced false-positive results, associated with thicker ill-defined dots, causing poor distinction between nonamplification and low amplification. ZytoDot CISH showed lower sensitivity, with increased frequency of false-positive results. CONCLUSIONS: SISH is the most reliable of the BDISH methods, with sensitivity and specificity highly comparable with FISH. It is also less deleterious than other BDISH methods, producing signals that were more distinct and therefore more readily analyzable even in poorly preserved tissue.

AB - AIMS: To evaluate the reliability of novel brightfield microscopy-based dual in situ hybridization (BDISH) methods for frontline HER2 status analysis in selected suboptimally preserved breast cancer tissue samples reflecting of the worst scenario in a community. METHODS AND RESULTS: A total of 320 morphologically poorly preserved breast invasive ductal carcinomas from the archives of 2 tertiary institutions in Brazil were selected for a tissue microarray-based analysis. 4B5 antibody was used for immunohistochemistry. Fluorescence in situ hybridization (FISH), DuoCISH, ZytoDot CISH, and silver in situ hybridization (SISH) were performed and compared. The highest agreement was observed between SISH and FISH. In addition, SISH was easier to assess in both amplified and nonamplified cases when compared with the other chromogenic methods, due to the sharpness of its dots. DuoCISH produced false-positive results, associated with thicker ill-defined dots, causing poor distinction between nonamplification and low amplification. ZytoDot CISH showed lower sensitivity, with increased frequency of false-positive results. CONCLUSIONS: SISH is the most reliable of the BDISH methods, with sensitivity and specificity highly comparable with FISH. It is also less deleterious than other BDISH methods, producing signals that were more distinct and therefore more readily analyzable even in poorly preserved tissue.

KW - breast cancer

KW - HER2

KW - in situ hybridization

KW - SISH

UR - http://www.scopus.com/inward/record.url?scp=84866617479&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84866617479&partnerID=8YFLogxK

U2 - 10.1097/PAS.0b013e3182635987

DO - 10.1097/PAS.0b013e3182635987

M3 - Article

VL - 36

SP - 1489

EP - 1496

JO - American Journal of Surgical Pathology

JF - American Journal of Surgical Pathology

SN - 0147-5185

IS - 10

ER -