Evolution of HBV S-gene in the backdrop of HDV co-infection

Samina Baig, Syed H. Abidi, Zahid Azam, Shahid Majid, Saeed Khan, Muhammad R. Khanani, Syed Ali

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

HBV-HDV co-infected people have a higher chance of developing cirrhosis, fulminant hepatitis, and hepatocellular carcinoma (HCC) compared to those infected only with HBV. The present study was conducted to investigate HBV genotypes and phylogeny among HBV mono-infected and HBV-HDV co-infected patients, as well as analyze mutations in the surface gene of HBV in mono-infected and co-infected patients. A total of 100 blood samples (50 co-infected with HBV and HDV, and 50 mono-infected with HBV only) were collected for this study. HBV DNA was extracted from patient sera and partial surface antigen gene was amplified from HBV genome using polymerase chain reaction. HBV S gene was sequenced from 49 mono-infected and 36 co-infected patients and analyzed to identify HBV genotypes and phylogenetic patterns. Subsequently, HBV S amino acid sequences were analyzed for mutational differences between sequences from mono- and co-infected patients. HBV genotype D was predominantly found in both mono-infected as well as co-infected patients. Phylogenetic analysis showed the divergence of HBV sequences, between mono- and co-infected patients, into two distinct clusters. HBV S gene mutation analysis revealed certain mutations in HBV-HDV co-infected subjects to be distinct from those found in mono-infected patients. This might indicate the evolution of HBV S gene under selection pressures generated from HDV coinfection.

Original languageEnglish
Pages (from-to)1328-1336
Number of pages9
JournalJournal of Medical Virology
Volume90
Issue number8
DOIs
Publication statusPublished - Aug 1 2018

Fingerprint

Coinfection
Genes
Genotype
Mutation
Phylogeny
Surface Antigens
Hepatitis
Amino Acid Sequence
Hepatocellular Carcinoma
Fibrosis
Genome
Pressure
Polymerase Chain Reaction
DNA
Serum

Keywords

  • evolution
  • hepatitis B virus
  • hepatitis D virus
  • mutation/mutation rate
  • virus classification

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Baig, S., Abidi, S. H., Azam, Z., Majid, S., Khan, S., Khanani, M. R., & Ali, S. (2018). Evolution of HBV S-gene in the backdrop of HDV co-infection. Journal of Medical Virology, 90(8), 1328-1336. https://doi.org/10.1002/jmv.25200

Evolution of HBV S-gene in the backdrop of HDV co-infection. / Baig, Samina; Abidi, Syed H.; Azam, Zahid; Majid, Shahid; Khan, Saeed; Khanani, Muhammad R.; Ali, Syed.

In: Journal of Medical Virology, Vol. 90, No. 8, 01.08.2018, p. 1328-1336.

Research output: Contribution to journalArticle

Baig, S, Abidi, SH, Azam, Z, Majid, S, Khan, S, Khanani, MR & Ali, S 2018, 'Evolution of HBV S-gene in the backdrop of HDV co-infection', Journal of Medical Virology, vol. 90, no. 8, pp. 1328-1336. https://doi.org/10.1002/jmv.25200
Baig S, Abidi SH, Azam Z, Majid S, Khan S, Khanani MR et al. Evolution of HBV S-gene in the backdrop of HDV co-infection. Journal of Medical Virology. 2018 Aug 1;90(8):1328-1336. https://doi.org/10.1002/jmv.25200
Baig, Samina ; Abidi, Syed H. ; Azam, Zahid ; Majid, Shahid ; Khan, Saeed ; Khanani, Muhammad R. ; Ali, Syed. / Evolution of HBV S-gene in the backdrop of HDV co-infection. In: Journal of Medical Virology. 2018 ; Vol. 90, No. 8. pp. 1328-1336.
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