Abstract
HBV-HDV co-infected people have a higher chance of developing cirrhosis, fulminant hepatitis, and hepatocellular carcinoma (HCC) compared to those infected only with HBV. The present study was conducted to investigate HBV genotypes and phylogeny among HBV mono-infected and HBV-HDV co-infected patients, as well as analyze mutations in the surface gene of HBV in mono-infected and co-infected patients. A total of 100 blood samples (50 co-infected with HBV and HDV, and 50 mono-infected with HBV only) were collected for this study. HBV DNA was extracted from patient sera and partial surface antigen gene was amplified from HBV genome using polymerase chain reaction. HBV S gene was sequenced from 49 mono-infected and 36 co-infected patients and analyzed to identify HBV genotypes and phylogenetic patterns. Subsequently, HBV S amino acid sequences were analyzed for mutational differences between sequences from mono- and co-infected patients. HBV genotype D was predominantly found in both mono-infected as well as co-infected patients. Phylogenetic analysis showed the divergence of HBV sequences, between mono- and co-infected patients, into two distinct clusters. HBV S gene mutation analysis revealed certain mutations in HBV-HDV co-infected subjects to be distinct from those found in mono-infected patients. This might indicate the evolution of HBV S gene under selection pressures generated from HDV coinfection.
| Original language | English |
|---|---|
| Pages (from-to) | 1328-1336 |
| Number of pages | 9 |
| Journal | Journal of Medical Virology |
| Volume | 90 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - Aug 1 2018 |
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Keywords
- evolution
- hepatitis B virus
- hepatitis D virus
- mutation/mutation rate
- virus classification
ASJC Scopus subject areas
- Virology
- Infectious Diseases
Cite this
Evolution of HBV S-gene in the backdrop of HDV co-infection. / Baig, Samina; Abidi, Syed H.; Azam, Zahid; Majid, Shahid; Khan, Saeed; Khanani, Muhammad R.; Ali, Syed.
In: Journal of Medical Virology, Vol. 90, No. 8, 01.08.2018, p. 1328-1336.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evolution of HBV S-gene in the backdrop of HDV co-infection
AU - Baig, Samina
AU - Abidi, Syed H.
AU - Azam, Zahid
AU - Majid, Shahid
AU - Khan, Saeed
AU - Khanani, Muhammad R.
AU - Ali, Syed
PY - 2018/8/1
Y1 - 2018/8/1
N2 - HBV-HDV co-infected people have a higher chance of developing cirrhosis, fulminant hepatitis, and hepatocellular carcinoma (HCC) compared to those infected only with HBV. The present study was conducted to investigate HBV genotypes and phylogeny among HBV mono-infected and HBV-HDV co-infected patients, as well as analyze mutations in the surface gene of HBV in mono-infected and co-infected patients. A total of 100 blood samples (50 co-infected with HBV and HDV, and 50 mono-infected with HBV only) were collected for this study. HBV DNA was extracted from patient sera and partial surface antigen gene was amplified from HBV genome using polymerase chain reaction. HBV S gene was sequenced from 49 mono-infected and 36 co-infected patients and analyzed to identify HBV genotypes and phylogenetic patterns. Subsequently, HBV S amino acid sequences were analyzed for mutational differences between sequences from mono- and co-infected patients. HBV genotype D was predominantly found in both mono-infected as well as co-infected patients. Phylogenetic analysis showed the divergence of HBV sequences, between mono- and co-infected patients, into two distinct clusters. HBV S gene mutation analysis revealed certain mutations in HBV-HDV co-infected subjects to be distinct from those found in mono-infected patients. This might indicate the evolution of HBV S gene under selection pressures generated from HDV coinfection.
AB - HBV-HDV co-infected people have a higher chance of developing cirrhosis, fulminant hepatitis, and hepatocellular carcinoma (HCC) compared to those infected only with HBV. The present study was conducted to investigate HBV genotypes and phylogeny among HBV mono-infected and HBV-HDV co-infected patients, as well as analyze mutations in the surface gene of HBV in mono-infected and co-infected patients. A total of 100 blood samples (50 co-infected with HBV and HDV, and 50 mono-infected with HBV only) were collected for this study. HBV DNA was extracted from patient sera and partial surface antigen gene was amplified from HBV genome using polymerase chain reaction. HBV S gene was sequenced from 49 mono-infected and 36 co-infected patients and analyzed to identify HBV genotypes and phylogenetic patterns. Subsequently, HBV S amino acid sequences were analyzed for mutational differences between sequences from mono- and co-infected patients. HBV genotype D was predominantly found in both mono-infected as well as co-infected patients. Phylogenetic analysis showed the divergence of HBV sequences, between mono- and co-infected patients, into two distinct clusters. HBV S gene mutation analysis revealed certain mutations in HBV-HDV co-infected subjects to be distinct from those found in mono-infected patients. This might indicate the evolution of HBV S gene under selection pressures generated from HDV coinfection.
KW - evolution
KW - hepatitis B virus
KW - hepatitis D virus
KW - mutation/mutation rate
KW - virus classification
UR - http://www.scopus.com/inward/record.url?scp=85048732243&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85048732243&partnerID=8YFLogxK
U2 - 10.1002/jmv.25200
DO - 10.1002/jmv.25200
M3 - Article
AN - SCOPUS:85048732243
VL - 90
SP - 1328
EP - 1336
JO - Journal of Medical Virology
JF - Journal of Medical Virology
SN - 0146-6615
IS - 8
ER -