Experimental model for studing the primary cilia in tissue culture cells

I. B. Alieva, L. A. Gorgidze, Yu A. Komarova, O. A. Chernobelskaya, I. A. Vorobjev

Research output: Contribution to journalArticle

Abstract

In HeLa, PK (pig kidney embryo cells), 3T3, PtK1 cells and rat embryo fibroblasts (REF), antibodies against acetylated tubulin stained centrioles, primary cilia, some cytoplasmic microtubules and microtubule bundles of the residual body. The primary cilia were stained more intensely than cytoplasmic microtubules and could easily distinguished from them. This makes it possible to detect the primary cilia in cultured cells and to estimate their number by light microscopy. The four studied cultures had 1/4 to 1/3 of interphase cells with detectable primary cilia, and only in HeLa cells the primary cilia were very rare. Comparison of electron microscopic and immunofluorescense data showed that the frequency of primary cilia in four tissue cultures determined by these two methods was the same. Therefore, one can use antibodies against acetylated tubulin for studying the primary cilia. In synchronized mitotic fibroblasts (3T3 and REF) the first primary cilia appeared 2 h after the cells had been plated on coverslips, which is 1 h after the cells had entered the interphase. Four hours after plating the number of ciliated cells reached the average level for nonsynchronous population. This model can be used for further investigation of the expression of primary cilia.

Original languageEnglish
Pages (from-to)716-717
Number of pages2
JournalBiologicheskie Membrany
Volume15
Issue number6
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Tissue culture
Cilia
Fibroblasts
Theoretical Models
Cell Culture Techniques
Tubulin
Rats
Antibodies
Plating
Microtubules
Optical microscopy
Embryonic Structures
Interphase
Cells
Electrons
Centrioles
3T3 Cells
HeLa Cells
Microscopy
Cultured Cells

ASJC Scopus subject areas

  • Biophysics
  • Clinical Biochemistry

Cite this

Alieva, I. B., Gorgidze, L. A., Komarova, Y. A., Chernobelskaya, O. A., & Vorobjev, I. A. (1998). Experimental model for studing the primary cilia in tissue culture cells. Biologicheskie Membrany, 15(6), 716-717.

Experimental model for studing the primary cilia in tissue culture cells. / Alieva, I. B.; Gorgidze, L. A.; Komarova, Yu A.; Chernobelskaya, O. A.; Vorobjev, I. A.

In: Biologicheskie Membrany, Vol. 15, No. 6, 1998, p. 716-717.

Research output: Contribution to journalArticle

Alieva, IB, Gorgidze, LA, Komarova, YA, Chernobelskaya, OA & Vorobjev, IA 1998, 'Experimental model for studing the primary cilia in tissue culture cells', Biologicheskie Membrany, vol. 15, no. 6, pp. 716-717.
Alieva IB, Gorgidze LA, Komarova YA, Chernobelskaya OA, Vorobjev IA. Experimental model for studing the primary cilia in tissue culture cells. Biologicheskie Membrany. 1998;15(6):716-717.
Alieva, I. B. ; Gorgidze, L. A. ; Komarova, Yu A. ; Chernobelskaya, O. A. ; Vorobjev, I. A. / Experimental model for studing the primary cilia in tissue culture cells. In: Biologicheskie Membrany. 1998 ; Vol. 15, No. 6. pp. 716-717.
@article{aeda985e30e74992b68a562b6d7abde1,
title = "Experimental model for studing the primary cilia in tissue culture cells",
abstract = "In HeLa, PK (pig kidney embryo cells), 3T3, PtK1 cells and rat embryo fibroblasts (REF), antibodies against acetylated tubulin stained centrioles, primary cilia, some cytoplasmic microtubules and microtubule bundles of the residual body. The primary cilia were stained more intensely than cytoplasmic microtubules and could easily distinguished from them. This makes it possible to detect the primary cilia in cultured cells and to estimate their number by light microscopy. The four studied cultures had 1/4 to 1/3 of interphase cells with detectable primary cilia, and only in HeLa cells the primary cilia were very rare. Comparison of electron microscopic and immunofluorescense data showed that the frequency of primary cilia in four tissue cultures determined by these two methods was the same. Therefore, one can use antibodies against acetylated tubulin for studying the primary cilia. In synchronized mitotic fibroblasts (3T3 and REF) the first primary cilia appeared 2 h after the cells had been plated on coverslips, which is 1 h after the cells had entered the interphase. Four hours after plating the number of ciliated cells reached the average level for nonsynchronous population. This model can be used for further investigation of the expression of primary cilia.",
author = "Alieva, {I. B.} and Gorgidze, {L. A.} and Komarova, {Yu A.} and Chernobelskaya, {O. A.} and Vorobjev, {I. A.}",
year = "1998",
language = "English",
volume = "15",
pages = "716--717",
journal = "Biologicheskie Membrany",
issn = "0233-4755",
publisher = "Russian Academy of Sciences",
number = "6",

}

TY - JOUR

T1 - Experimental model for studing the primary cilia in tissue culture cells

AU - Alieva, I. B.

AU - Gorgidze, L. A.

AU - Komarova, Yu A.

AU - Chernobelskaya, O. A.

AU - Vorobjev, I. A.

PY - 1998

Y1 - 1998

N2 - In HeLa, PK (pig kidney embryo cells), 3T3, PtK1 cells and rat embryo fibroblasts (REF), antibodies against acetylated tubulin stained centrioles, primary cilia, some cytoplasmic microtubules and microtubule bundles of the residual body. The primary cilia were stained more intensely than cytoplasmic microtubules and could easily distinguished from them. This makes it possible to detect the primary cilia in cultured cells and to estimate their number by light microscopy. The four studied cultures had 1/4 to 1/3 of interphase cells with detectable primary cilia, and only in HeLa cells the primary cilia were very rare. Comparison of electron microscopic and immunofluorescense data showed that the frequency of primary cilia in four tissue cultures determined by these two methods was the same. Therefore, one can use antibodies against acetylated tubulin for studying the primary cilia. In synchronized mitotic fibroblasts (3T3 and REF) the first primary cilia appeared 2 h after the cells had been plated on coverslips, which is 1 h after the cells had entered the interphase. Four hours after plating the number of ciliated cells reached the average level for nonsynchronous population. This model can be used for further investigation of the expression of primary cilia.

AB - In HeLa, PK (pig kidney embryo cells), 3T3, PtK1 cells and rat embryo fibroblasts (REF), antibodies against acetylated tubulin stained centrioles, primary cilia, some cytoplasmic microtubules and microtubule bundles of the residual body. The primary cilia were stained more intensely than cytoplasmic microtubules and could easily distinguished from them. This makes it possible to detect the primary cilia in cultured cells and to estimate their number by light microscopy. The four studied cultures had 1/4 to 1/3 of interphase cells with detectable primary cilia, and only in HeLa cells the primary cilia were very rare. Comparison of electron microscopic and immunofluorescense data showed that the frequency of primary cilia in four tissue cultures determined by these two methods was the same. Therefore, one can use antibodies against acetylated tubulin for studying the primary cilia. In synchronized mitotic fibroblasts (3T3 and REF) the first primary cilia appeared 2 h after the cells had been plated on coverslips, which is 1 h after the cells had entered the interphase. Four hours after plating the number of ciliated cells reached the average level for nonsynchronous population. This model can be used for further investigation of the expression of primary cilia.

UR - http://www.scopus.com/inward/record.url?scp=0040577174&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0040577174&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0040577174

VL - 15

SP - 716

EP - 717

JO - Biologicheskie Membrany

JF - Biologicheskie Membrany

SN - 0233-4755

IS - 6

ER -